A5107969416- Hearing, Cochlea, Tinnitus, Genetics
- Biochemical and Molecular Research
- Diet, Metabolism, and Disease
- Biotechnology and Related Fields
- Genomics and Rare Diseases
- Cancer-related gene regulation
- Glycosylation and Glycoproteins Research
- Metabolism and Genetic Disorders
- Parkinson's Disease Mechanisms and Treatments
- Biomedical Text Mining and Ontologies
- Genetics, Bioinformatics, and Biomedical Research
- Carbohydrate Chemistry and Synthesis
- Autism Spectrum Disorder Research
- Stress Responses and Cortisol
- Vestibular and auditory disorders
- Noise Effects and Management
- Toxin Mechanisms and Immunotoxins
- Oral Health Pathology and Treatment
- Biochemical effects in animals
- Tryptophan and brain disorders
- Mangiferin and Mango Extracts
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Biotin and Related Studies
- Microbial Metabolic Engineering and Bioproduction
University of Cape Town
2019-2024
University of Oxford
2024
Institute of Primate Research
2024
Centre for Human Genetics
2024
University of Ibadan
2015-2020
Stellenbosch University
2018-2020
Delta State University
2014-2015
Georgetown University
1968-1994
National Institutes of Health
1994
National Institute of Arthritis and Musculoskeletal and Skin Diseases
1963
Human A, B, and O erythrocytes (RBC) were agglutinated by many human strains of Actinomyces viscosus A. naeslundii. At 37 degrees C, these bacterium-mediated hemagglutination reactions required the action bacterial neuraminidase upon RBC; however, at 4 requirement for was not as striking. Bacterial cell suspensions which caused C contained both soluble extracellular cell-associated activities shown enzyme assays using a substrate (i.e., alpha 1-acid glycoprotein). Bacterium-mediated occurred...
The effects of oral administration low doses monosodium glutamate (MSG) on behavioral phenotypes, biomarkers oxidative stress in the brain and liver enzymes mice were evaluated this study. Mice treated orally with MSG (100, 250 500 mg/kg) daily for 21 days before testing phenotypes; memory, anxiety, spontaneous motor activity (SMA) depression. Thereafter, levels malondialdehyde (MDA) glutathione (GSH) as well activities enzymes, aminotransferase (AST) alanine (ALT) determined...
Abstract The reaction mechanism for the first biosynthetic step leading to formation of deoxy sugars has been studied with a specifically tritiated substrate, thymidine diphosphoglucose-4T. In presence partially purified TDP-glucose oxidoreductase from Escherichia coli B, substrate was converted quantitatively TDP-4-keto-6-deoxyglucose-6T. No exchange tritium medium occurred during reaction. Chemical degradation revealed that all originally present on carbon 4 glucose had migrated 6 4-keto...
Abstract TDP-Glucose oxidoreductase has been purified to the stage of homogeneity. Crystallization enzyme-protein was achieved without change its catalytic activity. The molecular weight, as determined by sedimentation equilibrium, found be 88,000. Kinetic data for assessment Km, competitive and noncompetitive inhibitors, activation energy are presented. homogeneous protein had 1 mole NAD+ tightly bound per enzyme; addition incubation mixtures did not increase enzymatic With sufficient...
Globally, across different cultures, humans have historically depended largely on medicinal plants for managing diseases that hitherto threatened their optimal health, survival, and longevity. Evidently, the health-derived benefits of been strongly attributed to presence secondary metabolites, particularly polyphenols. The potential health leaf sheaths West African variety Sorghum bicolor-based Jobelyn Supplement (SBJS) also ascribed its high contents This systematic review seeks...
Abstract Causal mutations in the MCPH1 gene have been associated with disorders like microcephaly, and recently congenital hearing impairment. This study examined DNA repair machinery identified genetic variations of interest gnomAD database to discuss biological roles effects rare variants MCPH1-related diseases. Notably, coordinates two seven known mechanisms which confirmed its neurogenesis chromatin condensation. A pathogenic missense variant p.Gly753Arg, frameshifts p.Asn189LysfsTer15...
Jobelyn® (JB) is an African sorghum-based food supplement claimed to be efficacious for the treatment of rheumatoid arthritis (RA). Although in vitro studies confirmed its anti-inflammatory property, no study had shown effect JB using vivo animal models inflammation. Thus, effects on acute and chronic inflammation rats were evaluated this study. Its rat red blood cell (RBC) lysis was also assessed.Acute induced with intraplanter injection carrageenan increase paw volume measured...
Abstract Background The prevalence of Parkinson’s disease (PD) is increasing in sub-Saharan Africa, but little known about the genetics PD these populations. Due to their unique ancestry and diversity, African populations have potential reveal novel insights into pathobiology PD. In this study, we aimed characterise genetic variation genes a group Black South Nigerian patients. Methods We recruited 33 14 patients, screened them for sequence variants 751 using an Ion AmpliSeq™ Neurological...
Hearing impairment (HI) is a common sensory disorder that defined as the partial or complete inability to detect sound in one both ears. This diverse pathology associated with myriad of phenotypic expressions and can be non-syndromic syndromic. HI caused by various genetic, environmental, and/or unknown factors. Some ontologies capture some forms, phenotypes, syndromes, but there no comprehensive knowledge portal which includes aspects specific disease state. hampers inter-study...
This study describes the roles of laboratory information management systems (LIMS) in multi-site genetics studies Africa. We used HiGeneS Africa project as a case study. The participants were recruited six African countries between 2019 to 2021. Baobab LIMS, server–client-based system (an African-led innovation) was for coordination biospecimen. development phase LIMS showcased team formation, data collection, biospecimen and shipment strategies. implementation registration, processing,...
Mouse models are used extensively to understand human pathobiology and mechanistic functions of disease-associated loci. However, in this review, we investigate the potential using genetic mouse identify markers that can disrupt hearing thresholds mice then target hearing-enriched orthologues loci humans. Currently, little is known about real prevalence genes cause impairment (HI) Africa. Pre-screening cell lines interest has improve diagnosis for HI Africa a significant percentage, example,...
Physiologically, the human and murine hearing systems are very similar, justifying extensive use of mice in experimental models for impairment (HI). About 340 HI genes have been reported; however, whether variants all human-mouse ortholog contribute to has rarely investigated. In humans, nearly 120 identified date, with GJB2 GJB6 accounting half congenital cases, genetic origin, populations European Asian ancestries, but not most African populations. The contribution other known among...
The ability of an organism to adapt aversive stressful situations or life challenging circumstances is very crucial its state health and survival. However, breakdown in adaptation due persistent uncontrollable stress, leads impairment bodily functions onset a variety pathological disorders especially memory decline. This study was designed evaluate the effect Jobelyn® (JB), potent antioxidant sorghum-based food supplement on unpredictable chronic mild stress (UCMS)-induced mice. Male Swiss...
Abstract Background: Recent studies in standard laboratories have indicated that a typical mango stem bark aqueous extract ( Magnifera indica Linn) possess anti-malaria and anti-fever properties. information also exists the literature, suggesting its potency as very effective anti-inflammatory plant extract. This study will therefore contribute immensely to systemic search for useful, less toxic natural bioactive medicinal compound. Objectives: investigated effects of Mangifera (MI)...
Objectives: To identify the etiologies of hearing impairment (HI) in schools for students who are deaf and to use a systematic review summarize reports on clinical genetic features HI Mali. Methods: We included individuals with that started before age 15 years old. Patients were carefully evaluated under standard practices, pure-tone audiometry was performed where possible. then searched articles published Malian population from databases' inception March 30, 2020. Results: A total 117 two...