A5102495424- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Fungal and yeast genetics research
- RNA Research and Splicing
- CAR-T cell therapy research
- Plant Virus Research Studies
- Corrosion Behavior and Inhibition
- Congenital heart defects research
- Magnesium Alloys: Properties and Applications
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Growth Hormone and Insulin-like Growth Factors
- Grey System Theory Applications
- Hydrogen Storage and Materials
- Aluminum Alloys Composites Properties
- Genetics and Neurodevelopmental Disorders
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Neural Networks and Reservoir Computing
- Connective tissue disorders research
- Nonlinear Dynamics and Pattern Formation
- Hepatitis B Virus Studies
Sun Yat-sen University
2020-2024
Nanchang University
2022-2024
Sun Yat-sen Memorial Hospital
2022-2023
Chongqing Technology and Business University
2023
Bridge University
2022
Rice University
2022
Baylor College of Medicine
2019-2022
Baylor University
2022
Institute of Microbiology
2018
Guangdong Provincial People's Hospital
2018
The major cell classes of the brain differ in their developmental processes, metabolism, signaling, and function. To better understand functions interactions types that comprise these classes, we acutely purified representative populations neurons, astrocytes, oligodendrocyte precursor cells, newly formed oligodendrocytes, myelinating microglia, endothelial pericytes from mouse cerebral cortex. We generated a transcriptome database for eight by RNA sequencing used sensitive algorithm to...
Spinal cord injury (SCI) is a devastating neurological disease without effective treatment. To generate comprehensive view of the mechanisms involved in SCI pathology, we applied RNA-Sequencing (RNA-Seq) technology to characterize temporal changes global gene expression after contusive mice. We sequenced tissue samples from acute and subacute phases (2 days 7 injury) systematically characterized transcriptomes with goal identifying pathways genes critical pathology. The top enriched...
The transcriptional intermediates of RNAs fold into secondary structures with multiple regulatory roles, yet the details such cotranscriptional RNA folding are largely unresolved in eukaryotes. Here, we present eSPET-seq (Structural Probing Elongating Transcripts eukaryotes), a method to assess Saccharomyces cerevisiae. Our study reveals pervasive structural transitions during and overall similarities between nascent mature RNAs. Furthermore, combined analysis genome-wide R-loop mutation...
We report the presence of second human pegivirus (HPgV-2) in Guangdong and Sichuan Provinces China. The prevalence HPgV-2 hepatitis C virus/HIV-1-co-infected persons who inject drugs was 12.9% 15.9% Sichuan. This population is at high risk for infection.
Pleiotropy, the phenomenon in which a single gene influences multiple traits, is fundamental concept genetics. However, evolutionary mechanisms underlying pleiotropy require further investigation. In this study, we conducted parallel knockouts targeting 100 transcription factors 2 strains of Saccharomyces cerevisiae. We systematically examined and quantified pleiotropic effects these on expression levels for each factor. Our results showed that knockout generally affected genes both strains,...
The activity of a gene newly integrated into chromosome depends on the genomic context integration site. This "position effect" has been widely reported, although other side coin, that is, how affects local chromosomal environment, remained largely unexplored, as have mechanism and phenotypic consequences this "externality" position effect. Here, we examined transcriptome profiles approximately 250 Saccharomyces cerevisiae strains, each with GFP different locus wild-type strain. We found in...
The objective of this study was to extend our previous research and further characterize the humoral immune responses against HIV-1 p24, gp41 specific peptides carrying immunodominant epitopes (IDEs) that react with human serum samples from HIV-1-infected individuals in China. We found majority (90.45%, 180/199) did not any three p24 IDEs, but recombinant full-length suggesting these tested China were primarily directed conformational p24. In contrast, 84.54% (164/194) reacted at least one...
Hematopoietic stem cells (HSCs) are used clinically for transplantation treatment to rebuild a patient's hematopoietic system in many diseases such as leukemia and lymphoma. Elucidating the mechanisms controlling HSCs self-renewal differentiation is important application of research clinical uses. However, it not possible obtain large quantity due their inability proliferate vitro. To overcome this hurdle, we mouse bone marrow derived cell line, EML (Erythroid, Myeloid, Lymphocytic) model...
Hematopoietic stem cells (HSCs) are used clinically for transplantation treatment to rebuild a patient's hematopoietic system in many diseases such as leukemia and lymphoma. Elucidating the mechanisms controlling HSCs self-renewal differentiation is important application of research clinical uses. However, it not possible obtain large quantity due their inability proliferate vitro. To overcome this hurdle, we mouse bone marrow derived cell line, EML (Erythroid, Myeloid, Lymphocytic) model...
To improve the prediction accuracy of carbon price, this paper proposes a combined model (CEEMDAN-SE-LiESN) based on complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN), sample entropy (SE), and leaky-integrator echo state networks (LiESN). First, price data are decomposed using CEEMDAN; then, modal components obtained from reconstructed to get three different sub-sequences; finally, sub-sequences predicted separately LiESN, final results by integration. The...
ABSTRACT When a gene is integrated into the chromosome, its activity depends on genomic context. Although this phenomenon of “position effect” was widely reported, how integration event affects local environment, or “externality” position effect, remained largely unexplored, let alone mechanism phenotypic consequence such externality. Here, we examined transcriptome profiles ∼250 Saccharomyces cerevisiae strains, each with GFP inserted different locus wild-type strain. We found that...
Abstract Adeno-associated virus (AAV) vector-based gene therapies can be applied to a wide range of diseases. AAV expression last for months years, but vector re-administration may necessary achieve life-long treatment. Unfortunately, immune system response against these vectors is potentiated after the first administration, which prevents clinical use repeated administration AAVs. Reducing AAVs while minimizing immunosuppression would improve delivery efficiency and long-term safety. In...
Abstract Pathogenic variants in the ZMIZ1 gene have been associated with neurodevelopmental disorder dysmorphic facies and distal skeletal anomalies (NEDDFSA). To date, only five reports identified mutations patients neuropsychiatric alterations. Here we described a Chinese pediatric patient intellectual disability (ID), early fast puberty, growth restriction, mild facial dysmorphisms, who had novel pathogenic variant (NM_020338.4:c.40C > T:p.Arg14*). Meanwhile, demonstrated molecular...