A5048171692- CAR-T cell therapy research
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- TGF-β signaling in diseases
- Cancer-related gene regulation
- Cellular Mechanics and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Bone Metabolism and Diseases
- Pancreatic and Hepatic Oncology Research
- Hippo pathway signaling and YAP/TAZ
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Protein Kinase Regulation and GTPase Signaling
- Adipose Tissue and Metabolism
- Osteoarthritis Treatment and Mechanisms
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Sarcoma Diagnosis and Treatment
- Bacterial Genetics and Biotechnology
- Muscle Physiology and Disorders
Southern Medical University
2021-2025
Guangzhou Medical University
2025
Guangzhou Blood Center
2025
Guangdong Medical College
2020-2023
Affiliated Hospital of Guangdong Medical College Hospital
2022
Chongqing University
2014-2021
University of California, San Diego
2019
ABSTRACT The proliferation of CAR‐T cells was hindered and cannot play its killing function well in solid tumors. And yet the regulatory mechanism cell is not fully understood. Here, we showed that recombinant expression CD19CAR T significantly increased basal activation level LCK activation. Both SMAD4 were essential for since over‐express or promotes proliferation, while knock‐down inhibited seriously. More go into apoptosis when expression, cycle arrested G2/M S phase, respectively....
Fyn kinase plays crucial roles in hematology and T cell signaling; however, there are currently limited tools to visualize the dynamic activity live cells. Here we developed characterized a highly sensitive biosensor based on fluorescence resonance energy transfer (FRET) monitor Our results show that can be induced both mouse embryonic fibroblasts (MEFs) cells by ligand engagement. Two different motifs were further introduced target at cellular membrane microdomains MEFs, revealing...
A new biosensor was engineered to visualize Lck kinase preactivation and regulation in live T cells.
CRIM1 is a member of the bone morphogenetic protein (BMP) antagonists; however, role in controlling cancer cell behavior remains unknown. This study investigated its function A549 line vitro. The results show that treating cells with peptide could increase migration and adhesion A549. Consistently, silencing expression decreased Furthermore, was increased which were treated transforming growth factor beta 1 to induced EMT. However, treatment N-CAD E-CAD expression. Finally, overexpression...
Alternative splicing is one of the most common mechanisms human gene regulation and plays a crucial role in increasing diversity functional proteins.Many diseases are linked to alternative splicing, especially cancer.SMAD4 member SMAD family critical mediating TGF-β signal transduction regulatory events.Smad4 tumour suppressor acts as shuttling protein between nucleus cytoplasm.The variants Smad4 have been found many cancers.The present study performed nested PCR detect HaCaT cells lines...
Introduction: Non-small cell lung cancer (NSCLC) is a worldwide malignance threatening human life. TGF-β/Smad signaling known to regulate proliferation, differentiation, migration and growth. As the only co-Smad playing crucial roles in TGF-β signaling, Smad4 reported be frequently mutated or occur as alternatively spliced tumor cells. was involved TGF-β-induced EMT process. However, whether alternative splicing occurs process NSCLC not clear. Methods: In our current study, we explored of...
Insulin-like growth factor 1 (IGF1) is a crucial factor, that regulates skeletal muscles development during cell and repair. Recently, its alternative splicing variant, named IGF1Ec, also mechano-growth (MGF), has gained attentions as new damage repair factor. However, the structure-function relationships of IGF1Ec have not been fully clarified due to contradictory reports. In this study, we systematically investigated physiologic responses C2C12 muscle cells IGF1 MGF E peptide. Our data...
Abstract Mechano‐growth factor (MGF), an alternative splicing variant of insulin‐like growth factor‐1 ( IGF‐1 ) gene, promotes cell proliferation and inhibits differentiation. It also plays important role in tumor development. is to optimize the production process achieve MGF protein because there no commercial available. In this study, human gene cloned into pGEX‐4T‐1 recombinant (rhMGF) could be expressed Rosetta (DE3) by isopropyl β‐ d ‐1‐thiogalactopyranoside induction but not BL21...
Osteosarcoma is frequently metastasized at the time of diagnosis in patients. However, underlying mechanism osteosarcoma metastasis remains poorly understood. In this study, we evaluated DNA methylation profiles combined with gene expression 21 patients metastatic and 64 non-metastatic from TARGET database identified PKIB AIM2 as hub genes related to osteosarcoma. To verify effects on migration invasion osteosarcoma, performed wound-healing assay transwell assay. The results showed that...