A5042384343- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Blood groups and transfusion
- Platelet Disorders and Treatments
- Blood donation and transfusion practices
- Blood disorders and treatments
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Erythrocyte Function and Pathophysiology
- HIV Research and Treatment
- Blood transfusion and management
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Brucella: diagnosis, epidemiology, treatment
- Systemic Lupus Erythematosus Research
- Immune cells in cancer
- Organ Donation and Transplantation
- Pediatric health and respiratory diseases
- HIV/AIDS drug development and treatment
- Virology and Viral Diseases
- Hepatitis Viruses Studies and Epidemiology
- Mesenchymal stem cell research
Southern Medical University
2017-2025
Guangzhou Blood Center
2016-2025
Guangzhou Medical University
2024-2025
Guangzhou First People's Hospital
2024-2025
Huazhong University of Science and Technology
2025
Jiangxi Academy of Forestry
2025
Guilin Medical University
2024
ShenZhen People’s Hospital
2020-2024
Soochow University
2023
First Affiliated Hospital of Soochow University
2023
// Ling Lin 1, 2, 3, * , Yong-Song Chen Yan-Dan Yao 2 Jing-Qi Jia-Ning Song-Yin Huang 4 Yun-Jie Zeng 5 He-Rui 6 Si-Hai 7 Yong-Shui Fu Er-Wei Song 1 Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-Sen Memorial Hospital, University, Guangzhou 510120, P. R. China Breast 3 Department Internal Medicine, the First Affiliated Shantou University College, 515041, Laboratory, Pathology, Oncology, Blood These authors have...
Abstract To determine hepatitis C virus (HCV) genotype distribution in China, a total of 148 HCV RNA positive serum samples were collected from nine geographic areas and subjected to RT‐PCR followed by direct DNA sequencing phylogenetic analysis the core, E1, NS5B regions. was genotyped 139 (93.9%) samples. Among them subtype 1b most predominant [66% (92/139)] 2a [14% (19/139)]. Of 92 isolates, 35 (38%) 30 (33%) formed two clusters, designated groups A B. Group prevalent throughout while...
As several vaccines for SARS-CoV-2 have been developed, a large proportion of individuals vaccinated worldwide so far. The rapid and accurate immunoassays are urgently needed detecting the specific virus-neutralizing antibody (NAb), which reflect protective effect among different populations.In this study, we designed quantum dot lateral flow immunoassay strip (QD-LFIA) smartphones detection IgG or neutralizing antibodies in human serum whole blood samples. recombinant receptor binding...
ABSTRACT The proliferation of CAR‐T cells was hindered and cannot play its killing function well in solid tumors. And yet the regulatory mechanism cell is not fully understood. Here, we showed that recombinant expression CD19CAR T significantly increased basal activation level LCK activation. Both SMAD4 were essential for since over‐express or promotes proliferation, while knock‐down inhibited seriously. More go into apoptosis when expression, cycle arrested G2/M S phase, respectively....
ABSTRACT Background and Aims Hepatitis C virus (HCV) vaccines are urgently needed to achieve WHO's goal for the elimination of viral hepatitis by 2030. The lack suitable animal models evaluating vaccine efficacy has greatly hindered development HCV vaccines. By using rat model chronically infected with rodent hepacivirus from Rattus norvegicus (RHV‐rn1), a homologously close as surrogate infection, we assessed protective effectiveness RHV‐rn1 Sad23L‐RHVns. Methods Sad23L‐RHVns was...
Summary. Recently, we studied hepatitis C virus (HCV) sera-prevalence among 559 890 first-time volunteer blood donors in China. From randomly selected 450 anti-HCV positive donors, detected HCV RNA 270 donors. In this study, amplified E1 and/or NS5B sequences from 236 of these followed by DNA sequencing and phylogenetic analysis. The results indicate new trends infection genotype distribution differed according to the donors' region origin. Among Guangdong province, subtypes 6a, 1b, 3a, 3b,...
Red blood cells (RBCs) of Asian-type DEL phenotype express few RhD proteins and are typed as serologic RhD-negative (D-) in routine testing. RhD-positive (D+) RBC transfusion for patients with has been proposed but not generally adopted because a lack direct evidence regarding its safety the underlying mechanism. We performed single-arm multicenter clinical trial to document outcome D+ DEL; none recipients (0/42; 95% confidence interval, 0-8.40) developed alloanti-D after median follow-up...
The tetrasaccharide 3-deoxy-alpha-D-manno-2-octulosonic acid (alpha-KDO) (2----8)-alpha-KDO(2----4)-alpha-KDO(2----6)-beta GlcNAc, a partial structure of chlamydial lipopolysaccharide (LPS) representing genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial glycoconjugate antigen. Mice were immunized with the prepare chlamydia-specific monoclonal antibodies. They selected LPS antigens structurally antigenically related Re-type...
MSCs (mesenchymal stem cells) with their versatile growth and differentiation potential are ideal candidates for use in regenerative medicine currently making way into clinical trials, which requires the development of xeno-free protocols culture. In this study, were cultured 10% FCS or 7.5% HPL (human platelet lysate)-supplemented media. We found that both groups showed a comparable morphology, phenotype proliferation. The percentage cells S- G2-/M-phases, however, was slightly up-regulated...
In China, high prevalence of HBV and HCV parallels with the growing epidemic syphilis HIV in general population poses a great threat to blood safety. This study investigated serologic markers for transfusion transmissible infections (TTIs) among four Chinese centers. We examined whole donations collected from January 2000 through December 2010 at Post-donation testing TTIs (HIV, HBV, syphilis) were conducted using two different enzyme-linked immunosorbent assay kits each seromarker. The (%)...
COVID-19 vaccines are being developed urgently worldwide. Here, we constructed two adenovirus vectored vaccine candidates of Sad23L-nCoV-S and Ad49L-nCoV-S carrying the full-length gene SARS-CoV-2 spike protein. The immunogenicity was individually evaluated in mice. Specific immune responses were observed by priming a dose-dependent manner, stronger obtained boosting. Furthermore, five rhesus macaques primed with 5 × 109 PFU Sad23L-nCoV-S, followed boosting at 4-week interval. Both mice well...
BACKGROUND: Antibodies against polymorphic structures on human neutrophil antigens (HNAs) play a role in alloimmune‐mediated neutropenia and are the leading cause of antibody‐mediated transfusion‐related acute lung injury (TRALI). This study aimed to determine frequencies HNAs major Han ethnic group living Guangdong Province, Southern China. STUDY DESIGN AND METHODS: A total 493 healthy Chinese blood donors from Guangzhou were recruited. DNA samples isolated typed for all five HNA‐1, ‐2, ‐3,...
Summary CD36 (also known as GPIV) deficiency is to be responsible for the production of anti-Naka antibodies in different clinical settings such fetal/neonatal alloimmune thrombocytopenia (FNAIT), platelet transfusion refractoriness (PTR) and post-transfusion purpura (PTP). However, no data regarding relevance immunisation currently available China. In this study, healthy blood donors were typed using flow cytometry. Nucleotide sequencing was performed identify molecular basis underlying...
Summary This study was carried out to determine the incidence of hepatitis B virus ( HBV ) infection in young generation born after mandatory implementation vaccination since 1992. Repeat blood donors between 1992 and 1997 were enrolled, who gave at least twice during past 3 years. Donors tested for markers HB sAg, anti‐ c, s viral DNA by immunoassays EIA s) nucleic acid tests NAT ). A total 14 937 pre‐donation screening qualified repeat aged 18‐23 years with 9 (0.06%) being sAg 10 (1:1494)...
We investigated the migration patterns of hepatitis C virus (HCV) in China. Partial E1 and/or NS5B sequences from 411 volunteer blood donors sampled 17 provinces and municipalities located five large regions, north-northeast, northwest, southwest, central south, southeast, were characterized. The classified into eight subtypes (1a, n = 3; 1b, 183; 2a, 83; 3a, 30; 3b, 44; 6a, 55; 6n, 10; 6v, 1) a new subtype candidate. Bayesian evolutionary analysis by sampling trees major revealed distinct...
Subtype 1b is the most common strain of Hepatitis C virus (HCV) in China. Here, molecular epidemiology and epidemic history this were investigated by conducting phylogenetic population genetic analyses E1 NS5B gene sequences sampled from nine Chinese cities. The analysis indicated presence two clusters strains that did not include reference other countries, suggesting these represent independent chains HCV transmission within remaining isolates more closely related to countries. date origin...
Different hepatitis C virus (HCV) genotypes exhibit differences in disease pathogenesis and progression, as well outcomes response to therapy. Tracking the change of HCV various epidemiological settings is critical for both surveillance development improved antiviral treatment. Here, we tracked changes prevalence China between 2004-2007 2008-2011. HCV-RNA-positive sera were collected from volunteer blood donors during period The determined by phylogenic analysis using NS5B E1 sequences....
We previously reported that sphingosine kinase 1 (SphK1), an enzyme catalyzes the production of sphingosine-1-phosphate (SIP), is upregulated in human gastric cancer and predicts poor clinical outcome. In present study, we used known differential effects UV irradiation on MGC-803 cells to determine their effect SphK1 activity. Ectopic expression markedly enhanced resistance irradiation, whereas silencing endogenous with shRNAs weakened this ability. Furthermore, these anti-apoptotic were...