A5046009219- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Fungal Biology and Applications
- Chromatography in Natural Products
- Phytochemistry and Biological Activities
- Phytochemicals and Antioxidant Activities
- Moringa oleifera research and applications
- Phytochemical compounds biological activities
- Berberine and alkaloids research
- Chemical synthesis and alkaloids
- Marine Sponges and Natural Products
- Ethnobotanical and Medicinal Plants Studies
- Carbohydrate Chemistry and Synthesis
- Analytical Chemistry and Chromatography
- Parkinson's Disease Mechanisms and Treatments
- Biofuel production and bioconversion
- Respiratory and Cough-Related Research
- Essential Oils and Antimicrobial Activity
- Alkaloids: synthesis and pharmacology
- Algal biology and biofuel production
- Bioactive Natural Diterpenoids Research
- Medicinal Plants and Neuroprotection
- Turfgrass Adaptation and Management
- Nutritional Studies and Diet
Owlstone Medical (United Kingdom)
2023-2024
Helwan University
2009-2023
Rothamsted Research
2018-2021
University of Strathclyde
2009-2021
A-T Society
2021
Molecular Discovery (United Kingdom)
2019-2020
Bangor University
2015-2019
Pharmac
2017
Metabolomics is a fast growing technology that has effectively contributed to many plant-related sciences and drug discovery.To use the non-targeted metabolomics approach investigate chemical profiles of three Malvaceae plants, namely Hibiscus mutabilis L. (Changing rose), H. schizopetalus (Dyer) Hook.f. (Coral Hibiscus), Malvaviscus arboreus Cav. (Sleeping along with evaluating their antioxidant anti-infective potential.Metabolic profiling was carried out using liquid chromatography coupled...
Bulb, leaf, scape and flower samples of British bluebells (Hyacinthoides non-scripta) were collected regularly for one growth period. Methanolic extracts freeze-dried ground showed antitrypanosomal activity, giving more than 50% inhibition, 20 out 41 samples. High-resolution mass spectrometry was used in the dereplication methanolic different plant parts. The results revealed differences chemical profile with bulb being distinctly from all aerial High molecular weight metabolites abundant...
Several approaches have been dedicated to activate the cryptic gene clusters in genomes of actinomycetes for targeted discovery new fascinating biomedical lead structures. In current study, N-acetylglucosamine was used maximize chemical diversity sponge-derived actinomycete Actinokineospora spheciospongiae sp. nov. HR-ESI-MS employed dereplication study and orthogonal partial least square-discriminant analysis applied evaluate data different fractions. As a result, two fridamycins H (1) I...
Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology that may find applications as pharmaceutical agrochemical drugs. In this study, combination metabolomics bioactivity-guided approaches were employed to isolate secondary metabolites cytotoxicity against cancer cells from an endophytic Aspergillus aculeatus. The endophyte was isolated the Egyptian plant Terminalia laxiflora identified using molecular biological methods. Metabolomics...
Euphorbia is a large genus of flowering plants with great diversity in metabolic pattern. Testing the cytotoxic potential fifteen species revealed highest activity E. officinarum L. against human colon adenocarcinoma (CACO2) cell line (IC50 7.2 µM) and lactea Haw. hepatoma (HepG2) breast (MCF-7) lines 5.2 5.1 µM, respectively). Additionally, profiling tested species, using LC-HRMS, for dereplication purposes, led to annotation 44 natural compounds. Among annotated compounds, diterpenoids...
Background and AimsThe prevalence of chronic liver disease in adults exceeds 30% some countries there is significant interest developing tests treatments to help control progression reduce healthcare burden. Breath a rich sampling matrix that offers non-invasive solutions suitable for early-stage detection monitoring. Having previously investigated targeted analysis single biomarker, here we multiparametric approach breath testing would provide more robust reliable results clinical use.
Endophytic fungi associated with medicinal plants are a potential source of novel chemistry and biology. Metabolomic tools were successfully employed to compare the metabolite fingerprints solid liquid culture extracts endophyte Curvularia sp. isolated from leaves Terminalia laxiflora. Natural product databases used dereplicate metabolites in order determine known compounds presence new natural products. Multivariate analysis highlighted putative responsible for bioactivity fungal extract...
With more than 156,000 described species, eukaryotic algae (both macro- and micro-algae) are a rich source of biological diversity, however their chemical diversity remains largely unexplored. Specialised metabolites with promising activities have been widely reported for seaweeds, recently extracts from microalgae exhibited activity in anticancer, antimicrobial, antioxidant screens. However, we still missing critical information on the distinction profiles between microalgae, as well space...
Two new flavonol glycosides, myricetin 4'-O-α-L-rhamnopyranoside (1) and quercetin 3'-O-α-L-rhamnopyranoside (2), together with a novel biflavonol compound, speciin (3), as well eleven phenolic metabolites, namely myricitrin (4), europetin 3-O-α-L-1C4-rhamnopyranoside (5), quercitrin (6), hyperin (7), rhamnetin 3-O-β-galacto-pyranoside (8), caffeic acid (9), methyl ester (10), chlorogenic (11), (12), gallic (13) (14), were identified from the 80 % methanol extract of aerial parts (leaves...
Abstract Background Cereal grains, including wheat ( Triticum aestivum L.), are major sources of food and feed, with being dominant in temperate zones. These end uses exploit the storage reserves starchy endosperm grain, starch component most cereal species. However, oats Avena sativa L.) differs that stores significant amounts oil. Understanding control carbon allocation between groups compounds, such as oil, is therefore important for understanding composition hence use quality cereals....
Brine, the historically known food additive salt solution, has been widely used as a pickling media to preserve flavor or enhance aroma, appearance, other qualities. The influence of pickling, using brine, on aroma compounds and primary secondary metabolite profile in onion bulb Allium cepa red cv. lemon fruit Citrus limon was evaluated multiplex metabolomics technologies. In lemon, negatively affected its key odor compound "citral", whereas monoterpene hydrocarbons limonene γ-terpinene...
There is an intense search for natural compounds that can inhibit the oligomerization and fibrillation of α-synuclein (α-Syn), whose aggregation key to development Parkinson's disease (PD). Rosa damascena a medicinal herb widely used in Middle Eastern food, ceremonies, perfumes. The known contain many different polyphenols. Here we investigated existence α-Syn inhibitors R. extract. Different HPLC fractions extract were assessed toxicity assays. most active led formation more oligomers but...
Tabebuia is the largest genus among family Bignoniaceae. species are known for their high ornamental and curative value. Here, cytotoxic potential of extracts from leaves stems five was analyzed. The highest activity observed T. rosea (Bertol.) DC. stem extract against HepG2 cell line (IC50 4.7 µg/mL), pallida L. MCF-7 6.3 pulcherrima CACO2 2.6 µg/mL). Metabolic profiling ten using liquid chromatography-high-resolution mass spectrometry dereplication purposes led to annotation forty...
The profiles of polar metabolites were determined in wholemeal flours grain from the Broadbalk wheat experiment and plants grown under organic low-input systems to study effects nutrition on composition. samples showed increased amino acids, acetate, choline decreased fructose succinate with increasing nitrogen fertilization. Samples receiving farm yard manure had similar those 96 kg N/ha but higher contents sugars, acids. A comparison only partial separation, clear climate agronomy....
Objectives: This study aimed to point the significant rule of metabolomics tools assess chemistry bioactive metabolites produced by endophytic fungus Aspergillus chevelieri isolated from Lagerostromia tomentosa C. presl. The anticancer and antimicrobial activity crude extracts, fractions pure compounds were investigated as part this study. Methods: An (Aspergillus chevelieri) was tissues stem presl identified through molecular biological procedure DNA isolation, PCR, sequencing searching...
Objectives: the aim of this study is to explore secondary metabolites produced by endophytic fungus Chaetomium sp. isolated from Scencio stapeliiformis (E. Phillips) as well investigate anticancer and antimicrobial activity crude extracts, fractions pure compounds. Methods: An (Chaetomium sp.) was aerial part S. (from Giza, Egypt). DNA sequencing analysis, morphological chemotaxonomy investigations were used for taxonomic identification. Metabolomics tools dereplication studies employed...
Mentha species are medicinally used worldwide and remain attractive for research due to the diversity of their phytoconstituents large therapeutic indices various ailments. This study metabolomics examination five (M. suaveolens, M. sylvestris, piperita, longifolia, viridis) justify cytotoxicity anti-Helicobacter effects. The activities were correlated with phytochemical profiles by orthogonal partial least square discriminant analysis (OPLS-DA). Tentatively characterized using liquid...
Cancer is a hazard life-threatening disease, which affect huge population worldwide. Marine actinomycetes are considered as promising source for potential chemotherapeutic agents. In our study, we carried out metabolic profiling Nocardia sp. UR 86 and Nocardiopsis 92 that were cultivated from the Red Sea sponge Amphimedon to investigate their chemical diversity using different media conditions. The crude culture extracts subjected high-resolution mass spectrometry (HRMS) analysis. profiles...
Azadirachta indica (neem), belonging to Meliaceae family and native Asia, has been used therapeutically in traditional Ayurveda, Unani, several local systems within Africa Chinese medicine treat different diseases for long times. The phytochemical screening of the seed oil these plants grown Nigeria resulted isolation nimbin from methanol soluble fraction hexane extract. structure this compound was established on basis nuclear magnetic resonance (NMR), Infrared (IR) mass (MS) spectroscopy....
This study aims to prioritize fungal strains recovered from under-explored habitats that produce new metabolites. HRMS dereplication is used avoid structure redundancy, and molecular modelling assign absolute configuration.MBC15-11F was isolated an amphipod identified using ITS, 28S, β-tubulin phylogeny as Aspergillus sydowii. Chemical profiling taxonomic-based structurally diverse metabolites, including unreported ones. Large-scale fermentation led the discovery of a N-acyl adenosine...