A5090918976- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Nanoparticle-Based Drug Delivery
- Proteins in Food Systems
- Ginkgo biloba and Cashew Applications
- Conducting polymers and applications
- Characterization and Applications of Magnetic Nanoparticles
- Cholinesterase and Neurodegenerative Diseases
- Flavonoids in Medical Research
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Bacterial biofilms and quorum sensing
- Electrochemical sensors and biosensors
- Iron oxide chemistry and applications
- Zebrafish Biomedical Research Applications
- Biochemical and biochemical processes
- Protein Hydrolysis and Bioactive Peptides
- Nanoparticles: synthesis and applications
- Erythrocyte Function and Pathophysiology
- Phagocytosis and Immune Regulation
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
- Nanomaterials for catalytic reactions
- Protein Interaction Studies and Fluorescence Analysis
- Nanocluster Synthesis and Applications
Technical University of Denmark
2022-2024
Aarhus University
2015-2022
Tarbiat Modares University
2015-2018
National Institute of Genetic Engineering and Biotechnology
2011-2018
Sharif University of Technology
2011-2014
Abstract The current understanding of the biological identity that nanoparticles may acquire in a given milieu is mostly inferred from hard component protein corona (HC). composition soft (SC) proteins and their relevance have remained elusive due to lack analytical separation methods. Here, we identify set specific with weak interactions at silica polystyrene by using an situ click-chemistry reaction. We show these SC are present also HC, but specifically enriched after capture, suggesting...
Aggregation of the natively unfolded protein α-synuclein (α-syn) is key to development Parkinson's disease (PD). Some nanoparticles (NPs) can inhibit this process and in turn be used for treatment PD. Using simulation strategies, we show here that α-syn self-assembly electrostatically driven. Dimerization by head-to-head monomer contact triggered dipole–dipole interactions subsequently stabilized van der Waals hydrogen bonds. Therefore, hypothesized charged nano-objects could interfere with...
Self-assembly of proteins to β-sheet rich amyloid fibrils is commonly observed in various neurodegenerative diseases. However, also occurs the extracellular matrix bacterial biofilm, which protects bacteria from environmental stress and antibiotics. Many Pseudomonas strains produce functional where main component highly fibrillation-prone protein FapC. FapC fibrillation may be inhibited by small molecules such as plant polyphenols, are already known inhibit formation pathogenic amyloid, but...
Co-delivery of anticancer drugs and biologics can provide synergetic effects outperform single delivery therapies. Here, a nanoparticle (NP) system for co-delivery methotrexate (MTX) STAT3 siRNA has been developed tested in vitro. Mesoporous silica nanoparticles (MSNs) were functionalized with chitosan (ch) by covalent grafting mediated aminopropyl triethoxysilane (APTES) via glutaraldehyde as the linker. MTX to MCF7 cells was demonstrated flow cytometric analysis confocal laser scanning...
A new super-branched amylopectin with longer exterior chains was produced from normal maize starch by modification branching enzyme followed 4-α-glucanotransferase, and applied for co-entrapment of a curcumin-loaded emulsion in alginate beads. The network structure the gel beads obtained Ca2+-cross-linked modest load retrograded starch. dual modified contained more α-1,6-linked branch than single unmodified starches showed superior resistance to digestive enzymes. Alginate or without were...
The interaction between nanoparticles (NPs) and the small intrinsically disordered protein α-synuclein (αSN), whose aggregation is central in development of Parkinson's disease, great relevance biomedical applications NPs as drug carriers. Here we showed using a combination different techniques that αSN interacts strongly with positively charged polyethylenimine-coated human serum albumin (PEI-HSA) NPs, leading to significant alteration secondary structure. In contrast, weak interactions HSA...
BackgroundIn recent years, there has been an increasing attention to the application of nanoparticles (NPs) in drug delivery.The interaction with cells and biomolecules, their biocompatibility are modulated by physicochemical properties NPs including size, shape, surface charge (1, 2).The size is important physical parameter complex effects on toxicity NPs.Human serum albumin (HSA) have gained considerable as delivery carriers, due advantages such being non-toxic, biodegrad-able, stable...
The mechanism of zwitterionic nanoliposomes to reduce α-synuclein aggregation and neurotoxicity.
Abstract The application of Fe 3 O 4 nanoparticles to the separation desulfurizing bacterial cells and their influence on desulfurization activity reusability two strains Rhodococcus erythropolis FMF R. IGTS8 were investigated. Magnetite synthesized via reverse coprecipitation method. Transmission electron microscopy (TEM) images showed that magnetite had sizes 5.35 ± 1.13 (F1 nanoparticles) 8.74 1.18 nm (F2 when glycine was added during synthesis it absent from reaction mixture,...
The aggregation of the 140-residue protein α-synuclein (αSN) plays a major role in pathogenesis different neurodegenerative disorders such as Parkinson's Disease (PD).
Nanoparticles (NPs) can modulate protein aggregation and fibril formation in the context of amyloid diseases. Understanding mechanism this action remains a critical next step developing nanomedicines for treatment or prevention Parkinson's disease. α-Synuclein (α-Syn) undergo interactions different strength with nanoparticles, these be prevented by presence corona (PC) acquired during exposure NPs to serum proteins. Here, we develop method attach PC irreversibly NPs, which enables us study...
The use of infant formulas (IFs) based on hydrolyzed cow's milk proteins to prevent allergy (CMA) is highly debated. risk sensitization induced by IFs may be affected the degree hydrolysis (DH) as well other physicochemical properties milk-based protein hydrolysates within IFs. immunogenicity (specific IgG1 induction) and sensitizing capacity IgE 30 whey- or casein-based with different characteristics were compared using an intraperitoneal model CMA in Brown Norway rats. In general,...
Abstract Nanoparticles are useful for increasing drug stability, solubility, and availability. The small molecule baicalein inhibits fibrillation, detoxifies aggregates of α‐synuclein (αSN) associated with Parkinson's disease (PD), but it suffers from instability, low solubility consequent Here is demonstrated that incorporation into zwitterionic nanoliposomes (NLP‐Ba) addresses these problems. NLP‐Ba αSN fibril initiation, elongation, secondary nucleation, also depolymerizes mature fibrils...
Abstract The present research comes up with a novel DNA‐loaded poly‐L‐lysine (PLL)/hyaluronan (HA) nanocarrier (DNA‐loaded PLL/HA NCs) for gene delivery applications, as promising candidate into diverse cells. A straightforward approach was employed to prepare such nanosystem through masking PLL molecules by HA. Fourier‐transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission‐scanning electron microscopy (FE‐SEM) and transmission (TEM) were used analyse the...
Amyloid fibrils typically consist of a dense core made up β-strands, with disordered flanks on either side, and sometimes interrupted by loop regions (the fuzzy coat). α-synuclein found in Lewy Bodies Parkinson’s Disease patients are mostly C-terminally truncated, meaning that large fraction the coat disease-related is enzymatically degraded amyloid state. We demonstrate proteolytic removal leads to enhanced fibril-fibril interactions flocculation, which renders study role bulk solution very...
The ability of proteins to aggregate form well-organized beta-sheet rich amyloid fibrils is increasingly viewed as a general if regrettable property the polypeptide chain. Aggregation leads diseases such amyloidosis and neurodegeneration in humans various mammalian species but also found functional variety both animals microbes. However, there are our knowledge no reports formation plants. Plants source large number aggregation-inhibiting compounds. We reasoned that two phenomena could be...
There is an intense search for natural compounds that can inhibit the oligomerization and fibrillation of α-synuclein (α-Syn), whose aggregation key to development Parkinson's disease (PD). Rosa damascena a medicinal herb widely used in Middle Eastern food, ceremonies, perfumes. The known contain many different polyphenols. Here we investigated existence α-Syn inhibitors R. extract. Different HPLC fractions extract were assessed toxicity assays. most active led formation more oligomers but...
Nanoparticles can acquire a biomolecular corona with species-specific biological identity. However, "non-self" incompatibility of recipient systems is often not considered, for example, when rodents are used as model organism preclinical studies biomolecule-inspired nanomedicines. Using zebrafish embryos an emerging nanobioimaging, here we unravel the in vivo fate intravenously injected 70 nm SiO2 nanoparticles protein preformed from fetal bovine serum (FBS), representing non-self Strikingly...