A5004238852- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
- Corneal surgery and disorders
- Oral microbiology and periodontitis research
- Alzheimer's disease research and treatments
- Blood properties and coagulation
- Streptococcal Infections and Treatments
- Bone and Dental Protein Studies
- Proteoglycans and glycosaminoglycans research
- Nanoparticles: synthesis and applications
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Blood Coagulation and Thrombosis Mechanisms
- Glaucoma and retinal disorders
- Platelet Disorders and Treatments
- Nanoparticle-Based Drug Delivery
- Liver Disease Diagnosis and Treatment
- Zebrafish Biomedical Research Applications
- interferon and immune responses
- Osteoarthritis Treatment and Mechanisms
- Enzyme Production and Characterization
- Polymer Surface Interaction Studies
- Marine and environmental studies
- Antimicrobial Peptides and Activities
Aarhus University
2016-2025
Danish Technological Institute
2023
University of Colorado Denver
2014
Institut de Biologie du Développement Marseille
2014
Aix-Marseille Université
2014
National Jewish Health
2014
Abstract The current understanding of the biological identity that nanoparticles may acquire in a given milieu is mostly inferred from hard component protein corona (HC). composition soft (SC) proteins and their relevance have remained elusive due to lack analytical separation methods. Here, we identify set specific with weak interactions at silica polystyrene by using an situ click-chemistry reaction. We show these SC are present also HC, but specifically enriched after capture, suggesting...
Proteins adsorbing at nanoparticles have been proposed as critical toxicity mediators and are included in ongoing efforts to develop predictive tools for safety assessment. Strongly attached proteins can be isolated, identified correlated changes nanoparticle state, cellular association or toxicity. Weakly attached, rapidly exchanging also present nanoparticles, but difficult isolate hardly examined. Here we study show the first time that they a strong modulatory effect on biotransformation...
Diseases of the cornea are common and refer to conditions like infections, injuries genetic defects. Morphologically, many corneal diseases affect only certain layers separate analysis individual is therefore interest explore basic molecular mechanisms involved in health disease. In this study, three main including, epithelium, stroma endothelium healthy human corneas were isolated. Prior by LC–MS/MS proteins from different either (i) separated SDS-PAGE followed in-gel trypsinization, (ii)...
Fermented dairy constitutes a major dietary source and contains lactose as the main carbohydrate living starter cultures, which can encounter intestinal microbiota after ingestion. To investigate whether dairy-related nutritional microbial modulation impacted composition activity, we employed static fecal fermentations model system consisting of Streptococcus thermophilus wild type β-galactosidase deletion mutant. In addition, conducted single-culture validation studies. 16S rRNA gene-based...
Evasion of killing by the complement system, a crucial part innate immunity, is key evolutionary strategy many human pathogens. A major etiological agent chronic periodontitis, Gram-negative bacterium Porphyromonas gingivalis, produces vast arsenal virulence factors that compromise defense mechanisms. One these peptidylarginine deiminase (PPAD), an enzyme unique to P. gingivalis among bacteria, which converts Arg residues in polypeptide chains into citrulline. Here, we report PPAD...
Cells recognize the biomolecular corona around a nanoparticle, but biological identity of complex may be considerably different among various species. This study explores importance protein composition for nanoparticle recognition by coelomocytes earthworm Eisenia fetida using E. coelomic proteins (EfCP) as native repertoire and fetal bovine serum (FBS) non-native reference. We have profiled forming long-lived silver nanoparticles (75 nm OECD reference materials) compared responses to...
Acne vulgaris is a very common disease of the pilosebaceous unit human skin. The pathological processes acne are not fully understood. To gain further insight sebaceous follicular casts were extracted from 18 healthy and 20 acne-affected individuals by cyanoacrylate-gel biopsies processed for mass spectrometry analysis, aiming at proteomic analysis casts. Human as well bacterial proteins identified. enriched in normal samples detected, respectively. Normal such prohibitins peroxiredoxins...
Hyaluronic acid (HA) and other glycosaminoglycans are extracellular matrix components in the human epidermis dermis. One of most prevalent skin microorganisms, Propionibacterium acnes, possesses HA-degrading activity, possibly conferred by enzyme hyaluronate lyase (HYL). In this study, we identified HYL P. acnes investigated genotypic phenotypic characteristics. Investigations include generation a acneshyl knockout mutant activity assays to determine substrate range formed products. We found...
Summary Carbamylation is a non-enzymatic post-translational modification induced upon exposure of free amino groups to urea-derived cyanate leading irreversible changes protein charge, structure and function. Levels carbamylated proteins increase significantly in chronic kidney disease albumin considered as an important biomarker indicating mortality risk. High plasma concentrations long half-life make fibrinogen prime target for carbamylation. As aggregation cross-linking fibrin monomers...
Data processing and analysis of proteomics data are challenging time consuming. In this paper, we present MS D ata M iner ( MDM ) http://sourceforge.net/p/msdataminer ), a freely available web‐based software solution aimed at minimizing the required for analysis, validation, comparison, presentation files generated in software, including ascot atrix S cience), istiller P rotein ilot AB ciex). The program was developed to significantly decrease process large proteomic sets publication. This...
Spiders are predaceous arthropods that capable of subduing and consuming relatively large prey items compared to their own body size. For this purpose, spiders have evolved potent venoms immobilise digestive fluids break down nutrients inside the prey's by means extra-oral digestion (EOD). Both secretions contain an array active proteins, overlap some components has been anecdotally reported, but not quantified. We systematically investigated extent such protein overlap. As venom injection...
Fuchs' endothelial corneal dystrophy (FECD) is a major disorder affecting the innermost part of cornea, leading to visual impairment. As morphological changes in FECD are mainly observed extracellular matrix Descemet's membrane/endothelial layer, we determined protein profiles diseased and control tissues using two relative quantitation MS methods. The first method, based on areas extracted ion chromatograms, quantified 51 48 most abundant proteins layer patient tissues, respectively, which...
Carbamylation of lysine residues and protein N-termini is an ubiquitous, non-enzymatic post-translational modification. at sites inflammation due to cyanate formation during the neutrophil oxidative burst may target within antimicrobial peptide LL-37. The bactericidal immunomodulatory properties LL-37 depend on its secondary structure cationic nature, which are conferred by arginine residues. Therefore, carbamylation affect biological functions present study examined kinetics pattern...
Abstract LL-37, the only human cathelicidin that is released during inflammation, a potent regulator of immune responses by facilitating delivery oligonucleotides to intracellular TLR-9, thereby enhancing response plasmacytoid dendritic cells (pDCs) extracellular DNA. Although important for pathogen recognition, this mechanism may facilitate development autoimmune diseases. In article, we show citrullination LL-37 peptidyl-arginine deiminases (PADs) hindered peptide-dependent DNA uptake and...
Sex- and species-specific biological identities of nanoparticles determine cellular recognition uptake by leukocytes in fish<italic>in vitro</italic>.
Cargo proteins of the type IX secretion system (T9SS) in human pathogens from Bacteroidetes phylum invariably possess a conserved C-terminal domain (CTD) that functions as signal for outer membrane (OM) translocation. In Porphyromonas gingivalis, CTD cargos is cleaved off after translocation, and anionic lipopolysaccharide (A-LPS) attached. This transpeptidase reaction anchors secreted to OM. PorZ, cell surface-associated protein, an essential component T9SS whose function was previously...
The enzyme extracellular superoxide dismutase (EC-SOD; SOD3) is a major antioxidant defense in lung and vasculature. A nonsynonomous single-nucleotide polymorphism EC-SOD (rs1799895) leads to an arginine glycine amino acid substitution at position 213 (R213G) the heparin-binding domain. In recent human genetic association studies, this attenuates risk of disease, yet paradoxically increases cardiovascular disease.Capitalizing on complete sequence homology between mouse domain, we created...
Inflammation is one of the hallmarks prostate cancer. The origin inflammation unknown, but microbial infections are suspected to play a role. In previous studies, Gram-positive, low virulent bacterium Cutibacterium (formerly Propionibacterium) acnes was frequently isolated from prostatic tissue. It unclear if presence represents true infection or contamination. Here we investigated type II, also called subspecies defendens, which most prevalent among C. isolates. Genome sequencing II...