A5100453244- RNA modifications and cancer
- Cancer-related gene regulation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- Bacterial Genetics and Biotechnology
- RNA regulation and disease
- Virus-based gene therapy research
- Biochemical Analysis and Sensing Techniques
- Microbial Community Ecology and Physiology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Antibiotic Resistance in Bacteria
- Physiological and biochemical adaptations
- Nuclear Receptors and Signaling
- Anesthesia and Neurotoxicity Research
- Plant Virus Research Studies
- Genetic Neurodegenerative Diseases
- CRISPR and Genetic Engineering
- Asthma and respiratory diseases
- MicroRNA in disease regulation
- Aquatic Ecosystems and Biodiversity
- Nerve injury and regeneration
- Intensive Care Unit Cognitive Disorders
- Protein Degradation and Inhibitors
Yunnan University
2020-2025
Chinese People's Liberation Army
2024
Vanderbilt University Medical Center
2024
Boston Children's Hospital
2018-2024
Harvard University
2018-2024
Harbin Medical University
2022-2024
Third Affiliated Hospital of Harbin Medical University
2022-2024
Nanjing Normal University
2022-2024
Guangdong Medical College
2024
Beijing Advanced Sciences and Innovation Center
2024
Dynamic and reversible RNA modifications such as N6-methyladenosine (m6A) can play important roles in regulating messenger (mRNA) splicing, export, stability translation. Defective mRNA modification through altered expression of the methyltransferase and/or demethylases results developmental defects cancer progression. Identifying modified mRNAs, annotating distribution sites across mRNA, well characterizing comparing other features are essential for studying function elucidating mechanism...
Abstract Cellular RNAs are subject to a myriad of different chemical modifications that play important roles in controlling RNA expression and function. Dysregulation certain modifications, the so-called ‘epitranscriptome’, contributes human disease. One limitation studying functional, physiological, pathological epitranscriptome is availability methods for precise mapping individual throughout transcriptome. 3-Methylcytidine (m3C) modification tRNAs well established was also recently...
Abstract The epitranscriptome includes a diversity of RNA modifications that influence gene expression. N3-methylcytidine (m 3 C) mainly occurs in the anticodon loop (position C32) certain tRNAs yet its role is poorly understood. Here, using HAC-Seq, we report comprehensive METTL2A/2B-, METTL6-, and METTL2A/2B/6-dependent m C profiles human cells. METTL2A/2B modifies tRNA-arginine tRNA-threonine members, whereas METTL6 tRNA-serine family. However, decreased C32 on tRNA-Ser-GCT isodecoders...
Abstract N 6 ‐methyladenosine (m A) is a novel epitranscriptomic marker that contributes to regulating diverse biological processes through controlling messenger RNA metabolism. However, it unknown if m A methylation affects uveal melanoma (UM) development. To address this question, we probed its function and molecular mechanism in UM. Initially, demonstrated global levels were dramatically elevated both UM cell lines clinical specimens. Meanwhile, found METTL3, main regulatory enzyme, was...
We demonstrate here that the assembly of RNase E‐based degradosome Escherichia coli is not required for normal mRNA decay in vivo . In contrast, deletion arginine‐rich RNA binding site (ARRBS) from E protein slightly impairs decay. When both scaffold region and ARRBS are missing, dramatically slowed, but 9S rRNA processing almost normal. An extensive truncation mutation ( rneδ610 ) had a more pronounced defect at 37°C than temperature‐sensitive rne‐1 allele 44°C. Taken together, these data...
Multidrug efflux pumps provide clinically significant levels of drug resistance in a number Gram-negative hospital-acquired pathogens. These pathogens frequently carry dozens genes encoding putative multidrug pumps. However, it can be difficult to determine how many these actually mediate antimicrobial efflux, and even more challenging identify the regulatory proteins that control expression In this study, we developed an innovative high-throughput screening method, combining transposon...
Salvianolic acid B (SalB), a bioactive compound isolated from the plant-derived medicinal herb Danshen, has been shown to exert various anti-oxidative and anti-inflammatory activities in several neurological disorders. In this study, we sought investigate potential protective effects associated molecular mechanisms of SalB Parkinson's disease (PD) models. To determine neuroprotective vitro, MPP+- or lipopolysaccharide (LPS)-induced neuronal injury was achieved using primary cultures with...
PCIF1 can mediate the methylation of N6,2'-O-dimethyladenosine (m6Am) in mRNA. Yet, detailed interplay between and potential cofactors its pathological significance remain elusive. Here, we demonstrated that PCIF1-mediated cap mRNA m6Am modification promoted head neck squamous cell carcinoma progression both vitro vivo. CTBP2 was identified as a cofactor to catalyze deposition on CLIP-Seq data bound similar mRNAs compared with PCIF1. We then used m6Am-Seq method profile site at single-base...
To investigate the function of AceR, a putative transcriptional regulator chlorhexidine efflux pump gene aceI in Acinetobacter baumannii.Chlorhexidine susceptibility and induction expression were determined by MIC quantitative real-time PCR, respectively, A. baumannii WT ΔaceR mutant strains. Recombinant AceR was prepared as both full-length protein truncated protein, (86-299), i.e. AceRt, which has DNA-binding domain deleted. The binding interaction purified its operator region investigated...
Human enterovirus 71 (EV-A71), a member of the Picornaviridae family, is predominantly associated with hand, foot, and mouth disease in infants young children. Additionally, EV-A71 can cause severe neurological complications, including aseptic meningitis, brainstem encephalitis, fatalities. The molecular mechanisms underlying these symptoms are complex involve viral tissue tropism, evasion from host immune responses, induction programmed cell death cytokine storms. This review article delves...
<title>Abstract</title> Lakes, reservoirs, and ponds are crucial inland water bodies that provide essential resources deliver significant ecological, social, cultural services. As a key indicator of quality, identifying the causes color changes is paramount importance. The periodic reddish-brown "pumpkin soup" phenomenon observed in Clean Pool (CP) Heilong (HP) southwestern China has raised concerns about quality ecosystem health. We used analytical approaches including, nutrient elements,...
Summary The rne gene of Escherichia coli encodes RNase E, an essential endoribonuclease that is involved in both mRNA decay and rRNA processing. Here we present evidence the transcribed from three promoters: p1, p2 p3. p3 promoters map 34 145 nt upstream previously characterized promoter, generating unusually long 5 ′ UTRs 395 506 respectively. Based on promoter– lacZ transcriptional fusions, p1 a more efficient promoter than either or Low copy number single vectors carrying alone complement...
Dyschromatosis symmetrica hereditaria (DSH) is an autosomal dominantly inherited skin disease associated with mutations of ADAR1, the gene that encodes a double-stranded RNA-specific adenosine deaminase. The purpose this study was to investigate potential in ADAR1 seven Chinese families DSH. All coding exons including adjacent intronic as well 5′ and 3′ untranslated region (UTR) were screened by direct sequencing. Moreover, quantitative reverse-transcription polymerase chain (qRT-PCR)...
Inflammatory bowel disease (IBD), including Crohn’s (CD) and ulcerative colitis (UC), is a chronic inflammatory affecting the colon, its incidence rising worldwide. Nur77, belongs to NR4A subfamily of nuclear hormone receptors, plays critical role in controlling pathology colitis. The aim this study investigate protection effect mechanism Gly-Pro-Ala (GPA) peptide, isolated from fish skin gelatin hydrolysate, mouse model dextran sulfate sodium- (DSS-) induced intestinal epithelial cells...