A5101598039- CRISPR and Genetic Engineering
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Aquaculture disease management and microbiota
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
- Biochemical Analysis and Sensing Techniques
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Zebrafish Biomedical Research Applications
- Physiological and biochemical adaptations
- Genetic diversity and population structure
- Virus-based gene therapy research
- Sperm and Testicular Function
- Fish Ecology and Management Studies
- Mitochondrial Function and Pathology
- melanin and skin pigmentation
- Reproductive Biology and Fertility
- Aquaculture Nutrition and Growth
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- Soft tissue tumor case studies
- High Altitude and Hypoxia
- Axial and Atropisomeric Chirality Synthesis
Hunan Normal University
2016-2025
Ministry of Education of the People's Republic of China
2024-2025
Nanjing Medical University
2024
Tongji Hospital
2023
Huazhong University of Science and Technology
2023
East China Normal University
2018-2019
Crucian carp is a popular ornamental strain in Asia with variants body color. To further explore the genetic mechanisms underlying gray and red color formation crucian carp, skin transcriptomes partial DNA methylation sites were obtained from (RCC) white (WCC). Here, we show significant differences mRNA expression between tissues of RCC WCC. Totals 3434 3683 unigenes had significantly lower higher WCC, respectively, compared expressed RCC. Some potential genes for development identified by...
Rapid genomic change has been demonstrated in several allopolyploid plant systems; however, few studies focused on animals. We addressed this issue using an allotetraploid lineage (4nAT) of freshwater fish originally derived from the interspecific hybridization red crucian carp (Carassius auratus var., ♀, 2n=100) × common (Cyprinus carpio L., ♂, 2n=100). constructed a bacterial artificial chromosome (BAC) library hybrids 20th generation (F20) and sequenced 14 BAC clones representing total...
Induced pluripotent stem (iPS) cells provide a powerful platform for the study of development, regeneration, and disease. Although many stable iPS cell lines have been established mammals, few attempts made to induce in nonmammalian species. Because technical advantages over other vertebrates on cells, induced from fish could be value research. In this paper, iPS-like were generated adult zebra fibroblasts by combining doxycycline inducible lentiviral delivery system chemical treatment....
Hypoxic stress causes cell damage and serious diseases in organisms, especially aquatic animals. It is important to elucidate the changes metabolic function caused by hypoxia mechanisms underlying these changes. This study focuses on low oxygen tolerance feature of a new blunt snout bream strain (GBSBF1). Our data show that GBSBF1 has different lipid carbohydrate metabolism pattern than wild-type bream, with altering glycolysis synthesis. In GBSBF1, expression levels phd2 vhl genes are...
Triploid crucian carp is a new breed of aquaculture with rapid growth characteristics. However, its regulation mechanism has not yet been elucidated. In this study, our results show that the expression levels mTOR signaling pathway-related genes (AKT1, AKT2, AKT3, mTOR, 4E-BP1 and S6K1) are higher in muscle intestine tissues triploid than those diploid red carp. Embryos exhibit faster rate comparing to And effect stage, mRNA pathway related carp, except for AKT2. Inhibition activity...
Previous research has indicated that triploid crucian carp (3n fish) have preferential resistance to cadmium (Cd) compared Carassius auratas red var . (2n fish). In this article, comparative is further conducted between the 2n and 3n fish in terms of immune response Cd-induced stress. Exposure 9 mg/L Cd for 96 h changed hepatic function indexes remarkably fish, but not fish. serum Cd-treated levels alanine amino transferase, aspartate aminotransferase, adenosine deaminase, total bilirubin...
The technique of induced pluripotent stem cells has significant application value in breeding and preserving the genetic integrity fish species. However, it is still unclear whether chemically can be from non-mammalian or not. In this article, we first verify that fibroblasts reprogrammed into cells. These stem-like possess features colony morphology, expression marker genes, formation embryoid bodies, teratoma formation, potential to differentiate germ cell-like vitro. Our findings will...
Pluripotency is a transient state in early embryos, which regulated by an interconnected network of pluripotency-related genes. The pluripotent itself seems to be highly dynamic, leads significant differences the description induced stem cells from different species at molecular level. With application cell reprogramming technology fish, establishment set standards for defining pluripotency will important research and potential fish. In this study, BLAST search expression pattern analysis,...
Polyploidy has many advantages over diploidy, such as rapid growth, sterility, and disease resistance, been extensively applied in agriculture aquaculture. Though generation of new polyploids via polyploidization achieved plants by different ways, it is comparatively rare animals. In this article, a chemical compound, SP600125, induced fish cells vitro, stable autotetraploid cell line generated from diploid fibroblast crucian carp. As c-Jun N-terminal kinase (Jnk) inhibitor, SP600125 does...
Cadmium is a major heavy metal pollutant. Even at low concentrations in waste water can accumulate algae and sediments, it absorbed by both plants aquatic animals, like shellfish fish. Triploid crucian carp an important economic fish, have been farmed on large scale China.to explore the molecular mechanisms underlying stress of triploid carp.we applied following experimental method to conduct research: transcriptomes analysis, histological observation, Quantitative real-time PCR enzyme...
Abstract Mitochondrial homoplasmy signifies the existence of identical copies mitochondrial DNA (mtDNA) and is essential for normal development, as heteroplasmy causes abnormal development diseases in human. Homoplasmy many organisms ensured by maternal mtDNA inheritance through either absence paternal delivery or early elimination mtDNA. However, whether transcribed has remained unknown. Here we report that shows late transcriptional quiescence cyprinid fishes. Paternal was present zygotes...
Background/Aims: Mitochondria (MT) and mitochondrial DNA (mtDNA) show maternal inheritance in most eukaryotic organisms; the sperm mtDNA is usually delivered to egg during fertilization then rapidly eliminated avoid heteroplasmy, which can affect embryogenesis. In our previous study, fertilization-delivered exhibited late elimination transcriptional quiescence cyprinid fish embryos. However, mechanisms underlying of paternal are unclear. Methods: Goldfish zebrafish were used investigate fate...
Androgenesis is a unique and rarely encountered reproductive mode in which the offspring only inherit paternal nuclear genome, resulting relatively few viable individuals. In this study, super male (YY) crucian carp was obtained by androgenesis with diploid sperm of autotetraploid (4n = 200). Flow cytometry assay confirmed fish diploid. The scanning electron microscopy flow analysis results revealed that YY produced unreduced sperm. To prove special characteristic homozygosity carp, three...
GCN2-eIF2α signaling pathway plays crucial roles in cell growth,development, and protein synthesis. However, polyploid fish, the function of this is rarely understood. In study, genes associated with (pkr, pek, gcn2, eif2α) are founded lower expression levels triploid crucian carp (3nCC) muscle compared to that red (RCC). effect stage embryos 3nCC, mRNA generally than those RCC, excluding hri fgf21. Inhibiting gcn2 3nCC downregulates downstream gene (eif2α, atf4, fgf21), accelerating...