A5103232177- Cell Adhesion Molecules Research
- Head and Neck Cancer Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Protease and Inhibitor Mechanisms
- Immune cells in cancer
- Radiomics and Machine Learning in Medical Imaging
- S100 Proteins and Annexins
- Platelet Disorders and Treatments
- Salivary Gland Disorders and Functions
- Liver Disease and Transplantation
- Lung Cancer Treatments and Mutations
- Salivary Gland Tumors Diagnosis and Treatment
- Liver Disease Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Blood Coagulation and Thrombosis Mechanisms
- Reconstructive Surgery and Microvascular Techniques
- Chemokine receptors and signaling
- RNA modifications and cancer
- Medical Imaging Techniques and Applications
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- Venous Thromboembolism Diagnosis and Management
- Cellular Mechanics and Interactions
- Ultrasound Imaging and Elastography
Ludwig-Maximilians-Universität München
2016-2025
LMU Klinikum
2016-2025
University Medical Center
2025
Dana-Farber Cancer Institute
2025
Cancer Research Center
2025
München Klinik
2015-2024
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde
2019-2022
Centre of Experimental Medicine of the Slovak Academy of Sciences
2009-2021
Institut für Hygiene und Umwelt
2020-2021
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde
2021
Significance Cell death by regulated necrosis causes tremendous tissue damage in a wide variety of diseases, including myocardial infarction, stroke, sepsis, and ischemia–reperfusion injury upon solid organ transplantation. Here, we demonstrate that an iron-dependent form necrosis, referred to as ferroptosis, mediates synchronized functional units diverse organs ischemia other stimuli, thereby triggering detrimental immune response. We developed novel third-generation inhibitor ferroptosis...
The aim of this study was to establish and validate a practical method disperse nanoparticles in physiological solutions for biological vitro vivo studies. TiO2 (rutile) dispersions were prepared distilled water, PBS, or RPMI 1640 cell culture medium. Different ultrasound energies, various dispersion stabilizers (human, bovine, mouse serum albumin, Tween 80, serum), concentrations stabilizers, different sequences preparation steps applied. size distribution dispersed analyzed by dynamic...
In AKI, dying renal cells release intracellular molecules that stimulate immune to secrete proinflammatory cytokines, which trigger leukocyte recruitment and inflammation. Whether the of histones, specifically, from contributes inflammation AKI is unknown. this study, we found tubular epithelial released histones into extracellular space, directly interacted with Toll-like receptor (TLR)-2 (TLR2) TLR4 induce MyD88, NF-κB, mitogen activated protein kinase signaling. Extracellular also had...
Crystals cause injury in numerous disorders, and induce inflammation via the NLRP3 inflammasome, however, it remains unclear how crystals cell death. Here we report that of calcium oxalate, monosodium urate, pyrophosphate dihydrate cystine trigger caspase-independent death five different types, which is blocked by necrostatin-1. RNA interference for receptor-interacting protein kinase 3 (RIPK3) or mixed lineage domain like (MLKL), two core proteins necroptosis pathway, blocks crystal...
Carcinoma cells undergo epithelial-mesenchymal transition (EMT); however, contributions of EMT heterogeneity to disease progression remain a matter debate. Here, we addressed the status ex vivo cultured circulating and disseminated tumor (CTCs/DTCs) in syngeneic mouse model metastatic breast cancer (MBC). Epithelial-type CTCs with restricted mesenchymal had strongest lung metastases formation ability, whereas mesenchymal-type showed limited ability. EpCAM expression served as surrogate...
Effective immune responses require the directed migration of leukocytes from vasculature to site injury or infection. How cells "find" their extravasation remains largely obscure. Here, we identified a previously unrecognized role platelets as pathfinders guiding exit points in microvasculature: upon onset inflammation, circulating were found immediately adhere at distinct sites venular microvessels enabling these cellular blood components capture neutrophils and, turn, inflammatory...
The major goal of radiotherapy is the induction tumor cell death. Additionally, can function as in situ cancer vaccination by exposing antigens and providing adjuvants for anti-tumor immune priming. In this regard, mode death repertoire released damage-associated molecular patterns (DAMPs) are crucial. However, optimal dosing fractionation remain controversial. Here, we examined initial steps priming different radiation regimens (20 Gy, 4 × 2 0 Gy) with lines triple-negative breast vitro...
Head and neck squamous cell carcinoma (HNSCC) remain a substantial burden to global health. Cell-free circulating tumour DNA (ctDNA) is an emerging biomarker but has not been studied sufficiently in HNSCC.We conducted single-centre prospective cohort study investigate ctDNA patients with p16-negative HNSCC who received curative-intent primary surgical treatment. Whole-exome sequencing was performed on formalin-fixed paraffin-embedded (FFPE) tissue. We utilised RaDaRTM, highly sensitive...
Abstract Background Epidermal growth factor receptor (EGFR) is both a driver oncogene and therapeutic target in advanced head neck squamous cell carcinoma (HNSCC). However, response to EGFR treatment inconsistent lacks markers for prediction. This study investigated EGFR-induced epithelial-to-mesenchymal transition (EMT) as central parameter tumor progression identified novel prognostic targets, candidate predictive marker therapy response. Methods Transcriptomic profiles were analyzed by...
The microvascular endothelium inherently controls nutrient delivery, oxygen supply, and immune surveillance of malignant tumors, thus representing both biological prerequisite therapeutic vulnerability in cancer. Recently, cellular senescence emerged as a fundamental characteristic solid malignancies. In particular, tumor endothelial cells have been reported to acquire senescence-associated secretory phenotype, which is characterized by pro-inflammatory transcriptional program, eventually...
Objective— Although the chemokines monocyte chemoattractant protein-1 (Ccl2/JE/MCP-1) and macrophage inflammatory protein-1α (Ccl3/MIP-1α) have recently been implicated in neutrophil migration, underlying mechanisms remain largely unclear. Methods Results— Stimulation of mouse cremaster muscle with Ccl2/JE/MCP-1 or Ccl3/MIP-1α induced a significant increase numbers firmly adherent transmigrated leukocytes (>70% neutrophils) as observed by vivo microscopy. This was significantly attenuated...
Despite their role in resolving inflammatory insults, neutrophils trigger inflammation-induced acute lung injury (ALI), culminating respiratory distress syndrome (ARDS), a frequent complication with high mortality humans. Molecular mechanisms underlying recruitment of to sites inflammation remain poorly understood. Here, we show that p38 MAP kinase p38δ is required for into sites. Global and myeloid-restricted deletion mice results decreased alveolar neutrophil accumulation attenuation ALI....
Fibronectin (FN), a major extracellular matrix component, enables integrin-mediated cell adhesion via binding of α5β1, αIIbβ3 and αv-class integrins to an RGD-motif. An additional linkage for α5 αIIb is the synergy site located in close proximity RGD motif. We report that mice with dysfunctional FN-synergy motif (Fn1syn/syn) suffer from surprisingly mild platelet bleeding defects due delayed thrombus formation after vessel injury. Additional loss β3 dramatically aggravates bleedings severely...
Chronic liver disease is the fifth most common cause of mortality in Europe. Recently, vitamin D deficiency has been associated with an increased risk general population. As patients advanced frequently exhibit deficiency, we assessed for a possible association survival cohort disease.Sixty-five cirrhosis (median age, 58 years; range, 19-76 66% male; Child-Pugh stage C, 46%) were included our prospective single-centre study. Serum 25-hydroxyvitamin concentrations measured by...
Endothelial dysfunction is a central pathomechanism in diabetes-associated complications. We hypothesized pathogenic role this of cathepsin S (Cat-S), cysteine protease that degrades elastic fibers and activates the protease-activated receptor-2 (PAR2) on endothelial cells. found injection mice with recombinant Cat-S induced albuminuria glomerular cell injury PAR2-dependent manner. In vivo microscopy confirmed for intrinsic Cat-S/PAR2 ischemia-induced microvascular permeability. vitro...
Abstract Breakdown of vascular barriers is a major complication inflammatory diseases. Anucleate platelets form blood-clots during thrombosis, but also play crucial role in inflammation. While spatio-temporal dynamics clot formation are well characterized, the cell-biological mechanisms platelet recruitment to micro-environments remain incompletely understood. Here we identify Arp2/3-dependent lamellipodia as prominent morphological feature immune-responsive platelets. Platelets use scan for...
Accurate risk-stratification can facilitate precision therapy in oropharyngeal squamous cell carcinoma (OPSCC). We explored the potential added value of baseline positron emission tomography (PET)/computed (CT) radiomic features for prognostication and risk stratification OPSCC beyond American Joint Committee on Cancer (AJCC) 8th edition staging scheme. Using institutional publicly available datasets, we included patients with known human papillomavirus (HPV) status, without distant...
Abstract Leukocyte infiltration of reerfused tissue is a key event in the pathogenesis ischemia-reperfusion. However, role chemokine receptors Ccr1, Ccr2, and Ccr5 for each single step postischemic recruitment process leukocytes has not yet been characterized. rolling, firm adherence, transendothelial, extravascular migration were analyzed cremaster muscle anaesthetized C57BL/6 mice using near-infrared reflected light oblique transillumination microscopy. Prior to 30 min ischemia as well at...
Human Alport disease is caused by a lack of the alpha3-, 4-, or 5-chain type IV collagen (COL4A). Affected humans and COL4A3-deficient mice develop glomerulosclerosis progressive renal fibrosis in presence interstitial macrophages, but their contribution to progression under debate. This question was addressed treating with BX471, an antagonist chemokine receptor 1 (CCR1) that known block leukocyte recruitment. Treatment BX471 from weeks 6 10 life improved survival mice, associated less...