A5100609021- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Enzyme-mediated dye degradation
- Genetically Modified Organisms Research
- Plant pathogens and resistance mechanisms
- Food composition and properties
- Agronomic Practices and Intercropping Systems
- Animal Genetics and Reproduction
- Plant Virus Research Studies
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Proteins in Food Systems
- Cocoa and Sweet Potato Agronomy
Chinese Academy of Agricultural Sciences
2015-2024
Biotechnology Research Institute
2015-2024
Institute of Animal Sciences
2023
Institute of Plant Protection
2021-2022
Shanghai Veterinary Research Institute
2022
Ministry of Agriculture and Rural Affairs
2022
Institute of Special Animal and Plant Sciences
2022
Shandong University
2021
Shanghai Jiao Tong University
2020
East China Normal University
2020
Plastic polymers are widely used in agriculture, industry, and our daily life because of their convenient economic properties. However, pollution caused by plastic polymers, especially polyethylene (PE), affects both animal human health when they aggregate the environment, as not easily degraded under natural conditions. In this study, Enterobacter sp. D1 was isolated from guts wax moth (Galleria mellonella). Microbial colonies formed around a PE film after 14 days cultivation with D1....
Growing populations and climate change pose great challenges to food security. Humankind is confronting a serious question: how will we feed the world in near future? This study presents an out-of-the-box solution involving highly efficient biosynthesis of artificial starch microbial proteins from available abundant agricultural residue as new sources. A one-pot biotransformation using vitro coenzyme-free synthetic enzymatic pathway baker's yeast can simultaneously convert dilute sulfuric...
Summary ALUMINUM‐ACTIVATED MALATE TRANSPORTER1 (ALMT1)‐mediated malate exudation from roots is critical for aluminium (Al) resistance in Arabidopsis. Its upstream molecular signalling regulation not yet well understood. The role of CALMODULIN‐LIKE24 (CML24) Al‐inhibited root growth and downstream ALMT1‐meditaed Al was investigated. CML24 confers demonstrated by an increased root‐growth inhibition the cml24 loss‐of‐function mutant under stress. This occurs mainly through ALMT1‐mediated roots....
Cucumber (Cucumis sativus L.) is characterized by its diverse and flexible sexual types. Here, we evaluated the effect of low temperature (LT) exposure on cucumber femaleness under short-day conditions. Shoot apices were subjected to whole-genome bisulfate sequencing (WGBS), mRNA-seq, sRNA-seq. The results showed that had a substantial global impact transposable element (TE)-related small RNA-directed DNA methylation (RdDM) mechanisms, resulting in large amounts CHH-type cytosine...
The fungal secondary metabolite patulin is a mycotoxin widespread in foods and beverages which poses serious threat to human health. However, no enzyme was known be able degrade this mycotoxin. For the first time, we discovered that manganese peroxidase (MrMnP) from Moniliophthora roreri can efficiently patulin. MrMnP gene cloned into pPICZα(A) then recombinant plasmid transformed Pichia pastoris X-33. strain produced extracellular with an activity of up 3659.5 U/L. rapidly patulin,...
Abstract Microbial levan is an important biopolymer with considerable potential in food and medical applications. Bacillus amyloliquefaciens NK-ΔLP strain can produce high-purity, low-molecular-weight levan, but production relatively low. To enhance the of six extracellular protease genes ( bpr , epr mpr vpr nprE aprE ), together tasA gene (encoding major biofilm matrix protein TasA) pgsBCA cluster (responsible for poly-γ-glutamic acid (γ-PGA) synthesis), were intentionally knocked out NK-1...
Cucumbers (Cucumis sativus) are known for their plasticity in sex expression. DNA methylation status determines gene activity but is susceptible to environmental condition changes. Thus, methylation-based epigenetic regulation may at least partially account the instability of cucumber Do temperature and photoperiod that two most important factors have equal effect on expression by similar mechanism? To answer this question, we did a two-factor experiment generated methylome transcriptome...
Delftia tsuruhatensis AD9 was isolated as an aniline-degrading bacterium from the soil surrounding a textile dyeing plant. The gene cluster involved in aniline degradation cloned total DNA of strain into Escherichia coli JM109. After shotgun cloning, two recombinant E. strains showing oxidation activity or catechol meta -cleavage were obtained by simple plate assays. These contained 9·3 kb and 15·4 fragments, respectively. Sequence analysis 24·7 region revealed that this contains (consisting...
We recently showed using prepro-orexin knockout (ORX-KO) mice and orexin neuron-ablated (ORX-AB) that neurons in the hypothalamus, but not peptides per se, are indispensable for stress-induced thermogenesis. To examine whether more generally involved central thermoregulatory mechanisms, we applied other forms of thermogenic perturbations, including brain prostaglandin E2 (PGE2) injections which mimic inflammatory fever environmental cold exposure, to ORX-KO mice, ORX-AB their wild-type (WT)...
Carotenoids are important pigments in plants that play crucial roles plant growth and responses to environmental stress. Lycopene β cyclase (β-LCY) functions at the branch point of carotenoid biosynthesis pathway, catalyzing cyclization lycopene. Here, a β-LCY gene from Nicotiana tabacum, designated as Ntβ-LCY1, was cloned functionally characterized. Robust expression Ntβ-LCY1 found leaves, obviously induced by salt, drought, exogenous abscisic acid treatments. Strong accumulation...
Aldehyde dehydrogenases (ALDH) is a family of enzymes including 19 members. For now, ALDH activity had been wildly used as marker cancer stem cells (CSCs). But biological functions relevant isoforms and their clinical applications are still controversial. Here, we investigate the significance potential function ALDH1A3 in gliomas. By whole-genome transcriptome microarray mRNA sequencing analysis, compared expression high- low- grade gliomas well different molecular subtypes. Microarray...
Abstract Seed coat mucilage plays an important role in promoting seed germination under adversity. Previous studies have shown that Arabidopsis thaliana MYB52 (AtMYB52) can positively regulate accumulation. However, the of Brassica napus ( BnaMYB52 ) accumulation and tolerance to osmotic stress during remains largely unknown. We cloned BnaA09.MYB52 coding domain sequence from B. cv ZS11, identified its conserved protein domains elucidated relationship with homologues a range plant species....
Abstract The fungus Humicola insolens is one of the most powerful decomposers crystalline cellulose. However, studies on β-glucosidases from this remain insufficient, especially glycosyl hydrolase family 3 enzymes. In present study, we analyzed functional diversity three distant strain Y1, which belonged to different evolutionary clades, by heterogeneous expression in Pichia pastoris GS115. recombinant enzymes shared similar enzymatic properties including thermophilic and neutral optima...
The maize ( Zea mays L.) in vivo maternal doubled haploid (DH) system is an important tool used by breeders and geneticists around the world. ability to rapidly produce DH lines of for breeding allows quickly respond new selection criteria based on ever‐changing biotic abiotic stresses that subjected across its growing area. There are two steps generation using system: (i) production identification progeny, (ii) doubling genomes create fertile, diploid inbred can be topcross per se...
Sclerotinia sclerotiorum (Lib.) de Bary, a destructive fungal pathogen with an extensive host range, causes various diseases the potential to cause huge economic losses crops worldwide. Streptomyces species produce secondary metabolites variable structures and biological activities that offer possible control methods for crop diseases. Herein, we evaluated inhibitory effects of wuyiencin, metabolite albulus CK-15, against S. sclerotiorum. The results showed wuyiencin markedly inhibited...
Niclosamide is commonly used as an antiparasitic drug in veterinary clinics. The objectives of this study were to evaluate the efficacy niclosamide against resistant Gram-positive bacteria vitro and vivo experimental model topical bacterial infection. Moreover, antibacterial mechanism Staphylococcus aureus.A mouse infection was established detect activity vivo. antimicrobial probed by visualizing morphologies using scanning electron microscopy transmission microscopy. haemolytic assay...