A5074851654- Cancer, Hypoxia, and Metabolism
- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Heat shock proteins research
- Pancreatic and Hepatic Oncology Research
- Prostate Cancer Treatment and Research
- Cancer Research and Treatments
- Medical Imaging Techniques and Applications
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Neuroblastoma Research and Treatments
- Signaling Pathways in Disease
- ATP Synthase and ATPases Research
- Colorectal Cancer Surgical Treatments
- Electron Spin Resonance Studies
- MicroRNA in disease regulation
- Gastric Cancer Management and Outcomes
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Anorectal Disease Treatments and Outcomes
- Pancreatitis Pathology and Treatment
- Lanthanide and Transition Metal Complexes
- Gastrointestinal disorders and treatments
- Digestive system and related health
Kyoto University
2010-2025
Kobe City Medical Center General Hospital
2009-2025
Tohoku Medical and Pharmaceutical University
2025
Kobe Medical Center
2024
Center for Cancer Research
2017-2022
National Institutes of Health
2018-2022
National Cancer Institute
2017-2022
Hokkaido University
2019
Obihiro University of Agriculture and Veterinary Medicine
2014
Kobe University
2014
Castration-resistant prostate cancer (CRPC) is characterized by reactivation of androgen receptor (AR) signaling, in part elevated expression AR splice variants (ARv) including ARv7, a constitutively active, ligand binding domain (LBD)-deficient variant whose has been correlated with therapeutic resistance and poor prognosis. In screen to identify small-molecule dual inhibitors both androgen-dependent androgen-independent gene signatures, we identified the chalcone C86. Binding studies using...
Altered cellular metabolism, including an increased dependence on aerobic glycolysis, is a hallmark of cancer. Despite the fact that this observation was first made nearly century ago, effective therapeutic targeting glycolysis in cancer has remained elusive. One potentially promising approach involves glycolytic enzyme lactate dehydrogenase (LDH), which overexpressed and plays critical role several cancers. Here, we used novel class LDH inhibitors to demonstrate, for time, Ewing sarcoma...
Abstract miRNAs have important roles in regulating cancer stem cell (CSC) properties and are considered to be potential therapeutic targets. However, few studies focused on which specifically related colon CSCs. Here, a PCR-based miRNA profiling analysis of normal cells (NCSC) CSCs (EpCAM+/CD44+/CD66a−) identified regulate CSC properties. Interestingly, miRNA-137 (miR-137) expression was downregulated the compared with NCSCs, while doublecortin-like kinase 1 (DCLK1) mRNA highly expressed but...
Cancer stem cells (CSCs) are considered to be responsible for the dismal prognosis of cancer patients. However, little is known about molecular mechanisms underlying acquisition and maintenance CSC properties in because their rarity clinical samples. We herein induced using defined factors. retrovirally introduced a set factors (OCT3/4, SOX2 KLF4) into human colon cells, followed by culture with conventional serum-containing medium, not embryonic cell medium. then evaluated cells. The...
Abstract Metabolic reprogramming is one of the defining features cancer and abnormal metabolism associated with many other pathologies. Molecular imaging techniques capable detecting such changes have become essential for diagnosis, treatment planning, surveillance. In particular, 18 F-FDG (fluorodeoxyglucose) PET has emerged as an modality because its unique ability to detect a disturbed molecular pathway through measurements glucose uptake. However, FDG-PET limitations that restrict...
Lactate dehydrogenase (LDH) catalyzes the conversion of pyruvate to lactate, with concomitant oxidation reduced nicotinamide adenine dinucleotide as final step in glycolytic pathway. Glycolysis plays an important role metabolic plasticity cancer cells and has long been recognized a potential therapeutic target. Thus, potent, selective inhibitors LDH represent attractive approach. However, date, pharmacological agents have failed achieve significant target engagement vivo, possibly because...
Metabolic differences among and within tumors can be an important determinant in cancer treatment outcome. However, methods for determining these non-invasively vivo is lacking. Using pancreatic ductal adenocarcinoma as a model, we demonstrate that tumor xenografts with similar genetic background distinguished by their differing rates of the metabolism 13C labeled glucose tracers, which imaged without hyperpolarization using newly developed techniques noise suppression. this method, subtypes...
Molecular imaging approaches for metabolic and physiologic of tumors have become important treatment planning response monitoring. However, the relationship between aspects is not fully understood. Here, we developed new hyperpolarized MRI electron paramagnetic resonance procedures that allow more direct assessment tumor glycolysis oxygenation status quantitatively. We investigated spatial hypoxia, glucose uptake, in three human pancreatic ductal adenocarcinoma xenografts with differing...
Dynamic nuclear polarization (DNP) is a cutting-edge technique that markedly enhances the detection sensitivity of molecules using magnetic resonance (NMR)/magnetic imaging (MRI). This methodology enables real-time dynamic metabolic status in vivo MRI. To expand targetable reactions, there demand for developing exogenous, i.e., artificially designed, DNP-NMR molecular probes; however, complying with requirements practical probes challenging because lack established design guidelines. Here,...
ABSTRACT Introduction Laparoscopic liver resection is an established surgical technique utilizing automatic staplers; however, the safety of extrahepatic Glissonean pedicle transection in this context remains unclear. This study evaluated and pressure resistance staple line reinforcement stapling using a porcine model. Methods The sheath was stapled with either Tri‐Staple or reinforcement, artificial blood infused into hepatic artery. Pressures were gradually increased up to 1000 mmHg assess...
Pyruvate is situated at the intersection of oxidative phosphorylation (OXPHOS) and glycolysis, which are primary energy-producing pathways in cells. Cancer therapies targeting these have been previously documented, indicating that inhibiting one pathway may lead to functional compensation by other, resulting an insufficient antitumor effect. Thus, effective cancer treatment necessitates concurrent comprehensive suppression both. However, whether a metabolic switch between occurs colorectal...
Abstract Drastic sensitivity enhancement of dynamic nuclear polarization is becoming an increasingly critical methodology to monitor real-time metabolic and physiological information in chemistry, biochemistry, biomedicine. However, the limited number available hyperpolarized 13 C probes, which can effectively interrogate crucial activities, remains one major bottlenecks this growing field. Here, we demonstrate [1- C] N -acetyl cysteine (NAC) as a novel probe for MRI glutathione redox plays...
In rectal surgery, double-stapled anastomosis is one of the most common techniques. However, crossing staple line considered a weakness this method and could lead to anastomotic leakage (AL), which major complications cancer surgery.To investigate usefulness laparoscopic intracorporeal reinforcement suturing for preventing AL in surgery.A total 153 patients with underwent resection using double-stapling technique between January 2015 December 2018. Patient characteristics, surgical data,...
Cancer stem cells (CSC) are capable of reconstructing cancer tissues, involved in both recurrence and metastasis, contribute to therapeutic resistance. Therefore, elucidating the molecular mechanism CSCs is important successfully treat unresectable cancers. Previously, we observed that colon stem-like can be induced from human cell lines by retrovirally introducing OCT3/4, SOX2, KLF4, have designated such as (iCSC). In current study, used iCSCs evaluate developed new methods control them....
Local recurrence after colorectal liver metastasis (CRLM) resection severely affects survival; however, the required surgical margin width remains controversial. This study investigated impact of KRAS status on and local rate (LRR) post-CRLM resection. Overall, 146 resected CRLMs with (wild-type (wtKRAS): 98, mutant (mKRAS): 48) were included. The LRR for each group, R1 (margin positive) R0 negative), was analyzed by status. further stratified into Ra ≥ 5 mm) Rb < mm). Patients had...
Abstract Vascular staplers are routinely used in laparoscopic liver resection, which has become a standard procedure advanced medical facilities. Although previous reports have outlined the benefits of staple line reinforcement (SLR), its application Glissonean pedicle transection during hepatic resection remains poorly studied. This study investigated surgical SLR as tool to enhance strength and improve perioperative hemostasis. Here, 10 patients who underwent using Tri‐StapleTM2.0...
Hepatic epithelioid hemangioendothelioma (HEHE) is a rare neoplasm of vascular origin. The clinical presentation HEHE variable, and the therapeutic criteria are still unclear since its natural history unpredictable. A 53-year-old woman was admitted to our hospital because she had 2.5-cm diameter nodule in segment V liver. She undergone segmental hepatectomy for solitary VII 10 years before. There been no recurrence after first operation. tumor diagnosed as new lesion by percutaneous needle...