A5083877308- Genomics, phytochemicals, and oxidative stress
- Natural product bioactivities and synthesis
- Heme Oxygenase-1 and Carbon Monoxide
- Seaweed-derived Bioactive Compounds
- Neuroinflammation and Neurodegeneration Mechanisms
- Bioactive natural compounds
- Pharmacological Effects of Natural Compounds
- Immune Response and Inflammation
- Air Quality and Health Impacts
- Ocular Surface and Contact Lens
- Fungal Infections and Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cutaneous lymphoproliferative disorders research
- Urinary Bladder and Prostate Research
- Nail Diseases and Treatments
- Vitamin C and Antioxidants Research
- Skin Protection and Aging
- Plant chemical constituents analysis
- Berberine and alkaloids research
- Hormonal and reproductive studies
- Inflammasome and immune disorders
- Phytochemical and Pharmacological Studies
- Phytochemicals and Antioxidant Activities
- Redox biology and oxidative stress
- Phytochemical compounds biological activities
Dong-Eui University
2015-2025
Pusan National University
2017-2025
National Institute of Biological Resources
2019
Maryknoll Medical Center
2015-2018
Kosin University
2016-2017
Kyung Hee University
2016
We examined the anti-cancer effect of genistein, a soy-derived isoflavone, in human bladder transitional cell carcinoma T24 cells. According to our data, genistein induced G2/M phase arrest cycle and apoptosis. Genistein down-regulated levels cyclin A B1, but up-regulated p21WAF1/CIP1, cyclin-dependent kinase (Cdk) inhibitor, that was complexed with Cdc2 Cdk2. Furthermore, activation caspases (caspase-3, -8 -9), cleavage poly (ADP-ribose) polymerase cleavage. However, genistein-induced...
It is evident based on literature that flavonoids from fruit can safely modulate cancer cell biology and induce apoptosis. Therefore, we investigated the anticancer activity of morin, a flavonoid which plentiful in twigs mulberry focusing apoptosis, its mechanisms. Morin upregulated Fas receptor, activates caspase-8, -9 -3 HCT-116 cells. also Bid, induced loss mitochondrial membrane potential (MMP, ∆Ψm) with Bax protein activation cytochrome c release. In addition, morin ROS generation was...
Although several studies have linked PM2.5 (particulate matter with a diameter less than 2.5 μm) to ocular surface diseases such as keratitis and conjunctivitis, very few previously addressed its effect on the retina. Therefore, aim of this study was evaluate epithelial-mesenchymal transition (EMT), process involved in disorders retinal pigment epithelial (RPE) APRE-19 cells. changed phenotype RPE cells from fibroblast-like mesenchymal, increased cell migration. Exposure markedly expression...
Licochalcone A (LCA) is a chalcone that predominantly found in the root of Glycyrrhiza species, which widely used as an herbal medicine. Although previous studies have reported LCA has wide range pharmacological effects, evidence for underlying molecular mechanism its anti-cancer efficacy still lacking. In this study, we investigated anti-proliferative effect on human bladder cancer cells, and induced cell cycle arrest at G2/M phase apoptotic death. Our data showed inhibited expression...
Coptisine is isoquinoline alkaloid derived from Coptidis Rhizoma and known to have potential anti-cancer activity toward various carcinomas. Targeting autophagy one of the main approaches for cancer therapy, but whether efficacy coptisine involves still unclear. Therefore, this study investigated effect on in hepatocellular carcinoma (HCC) Hep3B cells, identified underlying mechanism. Our results showed that increased cytotoxicity autophagic vacuoles a concentration-dependent manner....
Mammalian thioredoxin reductase (TrxR) plays a vital role in restoring cellular redox balance disrupted by reactive oxygen species (ROS) generation and oxidative damage. Here, we evaluated whether auranofin, selective inhibitor of TrxR, could serve as potential anti-cancer agent through its targeting TrxR activity Hep3B hepatocellular carcinoma cells. Auranofin treatment reduced the these cells induced apoptosis, which were accompanied up-regulation death receptors (DRs) activation caspases,...
Isorhamnetin is an O-methylated flavonol that predominantly found in the fruits and leaves of various plants, which have been used for traditional herbal remedies. Although several previous studies reported this has diverse health-promoting effects, evidence still lacking underlying molecular mechanism its anti-cancer efficacy. In study, we examined anti-proliferative effect isorhamnetin on human bladder cancer cells triggered gap 2/ mitosis (G2/M) phase cell arrest apoptosis. Our data...
Chronic inflammation, which is promoted by the production and secretion of inflammatory mediators cytokines in activated macrophages, responsible for development many diseases. Auranofin a Food Drug Administration-approved gold-based compound treatment rheumatoid arthritis, evidence suggests that auranofin could be potential therapeutic agent inflammation. In this study, to demonstrate inhibitory effect on chronic saturated fatty acid, palmitic acid (PA), low concentration lipopolysaccharide...
Mangiferin is a kind of natural xanthone glycosides and known to have various pharmacological activities. However, since the beneficial efficacy this compound has not been reported in retinal pigment epithelial (RPE) cells, study aimed evaluate whether mangiferin could protect human RPE ARPE-19 cells from oxidative injury mimicked by hydrogen peroxide (H
Oxidative stress contributes to the onset of chronic diseases in various organs, including muscles. Morroniside, a type iridoid glycoside contained
The thioredoxin (Trx) system plays critical roles in regulating intracellular redox levels and defending organisms against oxidative stress. Recent studies indicated that Trx reductase (TrxR) was overexpressed various types of human cancer cells indicating the Trx-TrxR may be a potential target for anti-cancer drug development. This study investigated synergistic effect auranofin, TrxR-specific inhibitor, on sulforaphane-mediated apoptotic cell death using Hep3B cells. results showed...
Non-alcoholic fatty liver disease (NAFLD) causes dysfunction and is associated with obesity type 2 diabetes. Chronic inflammation not only the development of NAFLD, but also hepatic diseases, including steatohepatitis, cirrhosis, hepatocellular carcinoma. Auranofin a treatment for rheumatoid arthritis has recently been reported to have potential effects against variety inflammation, cancer, viral infection. In this study, auranofin may be considered as new management metabolic syndrome, well...
In this study, we investigated the inhibitory effect of 5-aminolevulinic acid (ALA), a heme precursor, on inflammatory and oxidative stress activated by lipopolysaccharide (LPS) in RAW 264.7 macrophages estimating nitric oxide (NO), prostaglandin E2 (PGE2), cytokines, reactive oxygen species (ROS). We also evaluated molecular mechanisms through analysis expression their regulatory genes, further anti-inflammatory antioxidant efficacy ALA against LPS zebrafish model. Our results indicated...
Betulinic acid (BA) is a naturally occurring pentacyclic triterpenoid and generally found in the bark of birch trees (Betula sp.). Although several studies have been reported that BA has diverse biological activities, including anti-tumor effects, underlying anti-cancer mechanism bladder cancer cells still lacking. Therefore, this study aims to investigate anti-proliferative effect human cell lines T-24, UMUC-3, 5637, identify mechanism. Our results showed induced death are accompanied by...
Objective: To investigate the synergistic effects of auranofm and schisandrin A (SA) on cell proliferation inhibition apoptosis induction in human hepatocellular carcinoma Hep3B cells. Methods: Cell viability was assessed using MTT to determine auranofin SA. Three-dimensional (3D) culture models were used evaluate spheroid structure size. Apoptosis analyzed by flow cytometry for sub-G 1 populations, annexin V staining, Western blotting apoptotic markers. Reactive oxygen species (ROS)...
Evidence suggests that auranofin (AF) exhibits anticancer activity by inhibiting thioredoxin reductase (TrxR). Here, in this study, we have investigated the synergistic effects of AF and morin their mechanism for focusing on apoptosis Hep3B human hepatocellular carcinoma cells. We assessed activities annexin V/PI double staining, caspase, TrxR assay. Morin enhances inhibitory as well reducing cell viability. Annexin staining revealed morin/AF cotreatment induced apoptotic death. AF‐induced...
Hepatocellular carcinoma (HCC) has a high mortality rate worldwide, and treatment is very limited due to its recurrence low diagnosis rate, therefore there an increasing need develop more effective drugs treat HCC. Coptisine one of the isoquinoline alkaloids, it various pharmacological effects. However, evidence for molecular mechanism anticancer efficacy still insufficient. Therefore, this study investigated antiproliferative effect coptisine on human HCC Hep3B cells identified action...
Retinal pigment epithelial (RPE) cells occupy the outer layer of retina and perform various biological functions. Oxidative damage to RPE is a major risk factor for retinal degeneration that ultimately leads vision loss. In this study, we investigated role spermidine in hydrogen peroxide (H2O2)-induced oxidative stress model using human cells. Our findings showed 300 μM H2O2 increased cytotoxicity, apoptosis, cell cycle arrest G2/M phase, whereas these effects were markedly suppressed by 10...
Coptidis Rhizoma is the dried rhizome from Coptis chinensis Franch. that has been shown to have a number of beneficial pharmacological properties including antioxidant, anti-inflammatory, and anti-cancer effects. However, effects on hepatocellular carcinoma (HCC) remain unclear. In this study, we investigated ethanol extract (CR) in HCC Hep3B cells xenograft mouse model. Our results showed CR significantly inhibited cell growth induced apoptosis through increased expression Bcl-2 associated...
Isoalantolactone (IALT) is one of the isomeric sesquiterpene lactones isolated from roots Inula helenium L. IALT known to possess various biological and pharmacological activities, but its anti-cancer mechanisms are not well understood. The aim present study was investigate anti-proliferative effects in human hepatocellular carcinoma (HCC) cells evaluate potential mechanisms. Our results demonstrated that treatment concentration-dependently suppressed cell survival HCC Hep3B cells, which...