A5078453576- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Herpesvirus Infections and Treatments
- Parasitic Diseases Research and Treatment
- Coccidia and coccidiosis research
- Cytomegalovirus and herpesvirus research
- Veterinary medicine and infectious diseases
- Animal Nutrition and Physiology
- Parasite Biology and Host Interactions
- Vector-Borne Animal Diseases
- RNA Interference and Gene Delivery
- Parasites and Host Interactions
- Amoebic Infections and Treatments
- Vector-borne infectious diseases
- Gastrointestinal motility and disorders
- Peptidase Inhibition and Analysis
- Lanthanide and Transition Metal Complexes
- Ion Channels and Receptors
- Powdery Mildew Fungal Diseases
- Adenosine and Purinergic Signaling
- Helicobacter pylori-related gastroenterology studies
- bioluminescence and chemiluminescence research
- Pharmacological Effects and Assays
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
Ningxia University
2022-2024
United Imaging Healthcare (China)
2024
Nanjing Agricultural University
2012-2022
Shangqiu Normal University
2020
Northwest A&F University
2016-2018
With a worldwide distribution, Eimeria spp. could result in serious economic losses to the poultry industry. Due drug resistance and residues, there are no ideal drugs vaccines against food animals. In current study, bioinformatics approach was employed design multiepitope antigen, named NSLC protein, encoding antigenic epitopes of E. necatrix NA4, tenella SAG1, acervulina LDH, maxima CDPK. Thereafter, protective immunity protein along with five adjuvants two nanospheres laying chickens...
Caused by Toxoplasma gondii , toxoplasmosis has aroused great threats to public health around the world. So far, no effective vaccine or drug is commercially available, and demands for a safe therapeutic strategy have become more urgent. In current study, we constructed DNA encoding T. ribosomal P2 protein (TgP2) denoted as TgP2-pVAX1 plasmid. To improve immunoprotection, nanomaterial poly-lactic- co -glycolic acid (PLGA) chitosan were used delivery vehicle construct TgP2-pVAX1/PLGA...
The ultrastructure of the interstitial cells Cajal (ICC) has been examined in birds, but distribution these remains obscure because a suitable marker is lacking. In present study, identification and expression c-Kit-positive chicken intestine were demonstrated by means situ hybridization histochemistry c-Kit gene real-time quantitative PCR. Two types stained positive for mRNA. first group consisted spindle-shaped or bipolar identified as ICC. ICC found at variety locations: level myenteric...
Toxoplasma gondii (T. gondii) is an intracellular parasitic protozoan that can cause serious public health problems. However, there no effectively preventive or therapeutic strategy available for human and animals. In the present study, we developed a DNA vaccine encoding T. oxidoreductase from short-chain dehydrogenase/reductase family (TgSDRO-pVAX1) then entrapped in chitosan poly lactic-co-glycolic acid (PLGA) to improve efficacy. When encapsulated (TgSDRO-pVAX1/CS nanospheres) PLGA...
The infections of chicken coccidiosis impact the welfare chickens and economical production poultry. Eimeria mitis is ubiquitous in coccidiosis, E. infection can significantly affect productivity birds. Up to now, few efficient vaccines against have been reported, whereas recombinant subunit delivered by nanomaterials may elicit an encouraging outcome. Thus, this study, we chose 1a (Em1a) protein as candidate antigen generate Em1a preparations. (rEm1a) was encapsulated with poly...
Trichinellosis is a foodborne zoonotic disease caused by Trichinella spp., including spiralis. In the present study, T. spiralis membrane-associated progesterone receptor component-2 (Ts-MAPRC2) gene was cloned and characterized using protein sequencing analysis. Furthermore, expression, purification, immunoblot assay, binding ability with antibody, immunofluorescence assay were performed. A direct effect of (P4) mifepristone (RU486) on Ts-MAPRC2 determined in vitro cell culture that showed...
Anaplasma spp. are important tick-borne pathogens endangering the health of humans and various animals. Although several studies have reported infection in livestock China, little is known about impact production categories on occurrence species. In present study, PCR tools targeting 16S rRNA msp4 genes were applied to investigate prevalence 509 blood samples dairy (n = 249), cashmere 139), meat 121) goats from Shaanxi province. The was 58.5% (298/509) goats, significant differences (p <...
Background. Trichinellosis is a foodborne zoonotic disease caused by Trichinella spp., including spiralis. This parasitic ranks as seven of the most infectious in world. In this context, it important to develop vaccine that can combat Trichinellosis, especially for humans and pigs. would be an step preventing transmission. study, we focus on homology modelling, binding site prediction, molecular simulation techniques used explore association between spiralis membrane-associated progesterone...
Proteome-wide lysine acetylation has been documented in apicomplexan parasite Toxoplasma gondii and Plasmodium falciparum. Here, we conducted the first acetylome unsporulated oocysts (USO), sporulated 7 h (SO 7h), (SO), sporozoites (S), second generation merozoites (SMG) of Eimeria tenella through a 4D label-free quantitative technique. Altogether, 8532 sites on 2325 proteins were identified E. tenella, among which 5445 1493 quantified. In addition, 557, 339, 478, 248, 241, 424...
Trichinella spiralis (T. spiralis), a nematode parasite, is the major cause of Trichinellosis, zoonotic disease. A key role MAPR in reproductive system to maintain pregnancy. Previous studies found that antihormone drug design and vaccine therapy recombinant protein (rTs-MAPRC2) control T. infection. The current study investigates inhibitory effects different ratios antibodies against Ts-MAPRC2 on development muscle larvae (ML) newborn (NBL). First, we performed indirect immunofluorescence...
Theileriosis is an important tick-borne pathogen of livestock globally, causing severe reduction productivity and economic loss. To systematically investigate the prevalence species Theileria spp. in goats from Shaanxi province, a total 509 blood samples were collected dairy, cashmere meat 7 counties, examined by using microscopic examination nested PCR targeting SSU rRNA gene. Of them, 268 (52.7%, 268/509) positive for infection. The was closely associated with ages production categories....
Toxoplasma gondii (T. gondii) is the most common zoonotic protozoa and has infected about one-third of population worldwide. Recombinant epitopes encapsulated in nanospheres have advantages over traditional T. vaccines. For an efficient delivery system, poly (DL-lactide-co-glycolide) (PLGA) chitosan are frequently used biodegradable polymeric with strong safety profiles. In present study, we first expressed purified histone H2A1 using prokaryotic expression system. The effects recombinant...
Almost every warm-blooded animal can be an intermediate host for Toxoplasma gondii (T. gondii); there is still no efficient vaccine and medicine available T. infections. Detected on the surface of free tachyzoites gondii, ribosomal protein P2 (TgRPP2) has been identified as a target protection against toxoplasmosis. In present study, TgRPP2 was firstly expressed in prokaryotic expression system, purified recombinant (rTgRPP2) characterized by its modulation effects murine macrophages. Then,...
The pathogen of toxoplasmosis, Toxoplasma gondii (T. gondii), is a zoonotic protozoon that can affect the health warm-blooded animals including humans. Up to now, an effective vaccine with completely protection still inaccessible. In this study, DNA encoding T. histone deacetylase SIR2 (pVAX1-SIR2) was constructed. To enhance efficacy, chitosan and poly (d, l-lactic-co-glycolic)-acid (PLGA) were employed design nanospheres loaded vaccine, denoted as pVAX1-SIR2/CS pVAX1-SIR2/PLGA nanospheres....
As an important zoonotic protozoan, Toxoplasma gondii (T. gondii) has spread around the world, leading to infections in one-third of population. There is still no effective vaccine or medicine against T. gondii, and recombinant antigens entrapped within nanospheres have benefits over traditional vaccines. In present study, we first expressed purified proteasome subunit alpha type 1 (TgPSA1), then encapsulated TgPSA1 (rTgPSA1) chitosan (CS nanospheres, rTgPSA1/CS nanospheres) incomplete...
HomePlant DiseaseVol. 105, No. 3First Report of Powdery Mildew Caused by Podosphaera fusca on Helianthus tuberosus in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst ChinaD. L. Pei, Q. C. Zhang, Y. Guo, X. Wang, and Z. YuD. Pei†Corresponding author: D. Pei; E-mail Address: [email protected]http://orcid.org/0000-0002-8985-5835Henan Provincial Engineering Research Center for Development Application Characteristic Microorganism Resources, Key Laboratory Plant-Microbe Interactions,...
Trichinella spiralis (T. spiralis), a nematode parasite, is the major cause of Trichinellosis, zoonotic disease. A key role MAPR in reproductive system to maintain pregnancy. Previous studies found antihormone (P4 and RU486) drug design vaccine therapy recombinant protein (rTs-MAPRC2) control T. infection. The current study investigates inhibitory effects different ratios antibody against Ts-MAPRC2 on development muscle larvae (ML) newborn (NBL). First, we performed indirect...
Toxoplasma gondii ( T. ) is a zoonotic disease that poses great harm to humans and animals. So far, no effective vaccine has been developed provide fully protection against such parasites. Recently, numerous researches have focused on the use of poly-lactic-co-glycolic acid (PLGA) chitosan (CS) for vaccines infections. In this study, we employed PLGA CS as vehicles ribosome protein (TgRPS2) delivery. TgRPS2-PLGA TgRPS2-CS nanospheres were synthesized by double emulsion solvent evaporation...
There is a need for faster amyloid PET scans to reduce patients' discomfort, minimize movement artifacts, and increase throughput. The recently introduced uMI Panorama PET/CT system featuring enhanced spatial resolution sub-200ps TOF offers the potential shorter scan duration without sacrificing image quality or efficacy detect Aβ deposition. study aims establish acquisition protocol [18F]florbetapir imaging using digital scanner Panorama, while ensuring adequate amyloid-β (Aβ) detectability...