A5080528601- Monoclonal and Polyclonal Antibodies Research
- Flavonoids in Medical Research
- Crystallization and Solubility Studies
- Pharmacogenetics and Drug Metabolism
- X-ray Diffraction in Crystallography
- Radioactive contamination and transfer
- Biosimilars and Bioanalytical Methods
- HER2/EGFR in Cancer Research
- Metabolomics and Mass Spectrometry Studies
- Radioactive Decay and Measurement Techniques
- Advanced Biosensing Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Nuclear Physics and Applications
- Computational Drug Discovery Methods
- Traditional Chinese Medicine Analysis
- Radioactivity and Radon Measurements
- Peroxisome Proliferator-Activated Receptors
- Biochemical and Molecular Research
- Phytochemistry and Biological Activities
- Alzheimer's disease research and treatments
- Eicosanoids and Hypertension Pharmacology
- Algal biology and biofuel production
- Immune Cell Function and Interaction
- Mitochondrial Function and Pathology
- Lipid metabolism and disorders
United States Food and Drug Administration
2013-2025
Aerospace Center Hospital
2025
Biogen (United States)
2019-2024
Second Hospital of Shandong University
2024
Southwest Medical University
2023
Institute of Process Engineering
2018-2022
Janssen (United States)
2022
Roche (Switzerland)
2022
Novartis (Switzerland)
2022
Novartis Institutes for BioMedical Research
2022
The reactive thiol of cysteine is often used for coupling maleimide-containing linker-payloads to antibodies resulting in the generation antibody drug conjugates (ADCs). Currently, a numbers ADCs development are made by linker-payload native or engineered residues on antibody. An ADC conjugated via hinge-cysteines an auristatin payload was as model this study understand impact maleimide linkers stability. trastuzumab protease-cleavable linker,...
The formation of 15-oxo-5,8,11,13-(<i>Z</i>,<i>Z</i>,<i>Z</i>,<i>E</i>)-eicosatetraenoic acid (15-oxo-ETE) as a product from rabbit lung 15-hydroxyprostaglandin dehydrogenase (PGDH)-mediated oxidation 15(<i>S</i>)-hydroperoxy-5,8,11,13-(<i>Z</i>,<i>Z</i>,<i>Z</i>,<i>E</i>)-eicosatetraenoic was first reported more than 30 years ago. However, the pharmacological significance 15-oxo-ETE has never been established. We have now evaluated 15-lipoxygenase (LO)-1-mediated arachidonic (AA) metabolism...
Abstract Background Chronic inflammation and metabolic dysfunction are key features of systemic aging, closely associated with the development progression age-related diseases. Bazi Bushen (BZBS), a traditional Chinese medicine used to alleviate frailty, delays biological aging by modulating DNA methylation levels. However, precise mechanism its anti-aging effect remains unclear. In this study, we developed Energy Expenditure Aging Index (EEAI) estimate age. By integrating EEAI transcriptome...
The 2017 11th Workshop on Recent Issues in Bioanalysis (11th WRIB) took place Los Angeles/Universal City, California 3–7 April with participation of close to 750 professionals from pharmaceutical/biopharmaceutical companies, biotechnology contract research organizations and regulatory agencies worldwide. WRIB was once again a 5-day, weeklong event – full immersion week bioanalysis, biomarkers immunogenicity. As usual, it specifically designed facilitate sharing, reviewing, discussing...
Aim: The application of high-resolution MS to antibody–drug conjugate (ADC) drug development may provide insight into their safety and efficacy. Quantification unconjugated cytotoxic (payload) characterization drug-to-antibody ratio distribution were determined in plasma using orthogonal acceleration quadrupole-time-of-flight MS. Results: Unconjugated payload quantification by quadrupole-time-of-flight-based MRMhighresolution triple quadrupole-based multiple reaction monitoring comparable...
As antibody-drug conjugate (ADC) design is evolving with novel payload, linker, and conjugation chemistry, the need for sensitive precise quantitative measurement of conjugated payload to support pharmacokinetics (PK) in high demand. Compared ADCs containing noncleavable linkers, a strategy specific linkers which are liable pH, chemical reduction, or enzymatic cleavage has gained popularity recent years. One bioanalytical approach take advantage this type linker development PK assay...
Abstract This study aimed to evaluate the pharmacokinetic characteristics, safety, and bioequivalence of 2 formulations fluticasone nasal spray in healthy Chinese subjects. A single‐center, randomized, open‐label, single‐dose, 2‐formulation, 2‐sequence, 2‐period crossover was conducted under fasting conditions. total 120 male female subjects were enrolled, which 119 completed entire study. The main parameters parent drug, propionate (FP), plasma as follows: For test formulation, maximum...
The amyloid-<i>β</i> peptide (A<i>β</i>)—in particular, the 42–amino acid form, A<i>β</i>1-42—is thought to play a key role in pathogenesis of Alzheimer's disease (AD). Thus, several therapeutic modalities aiming inhibit A<i>β</i> synthesis or increase clearance have entered clinical trials, including <i>γ</i>-secretase inhibitors, anti-A<i>β</i> antibodies, and precursor protein cleaving enzyme inhibitors. A unique class small molecules, modulators (GSMs), selectively reduce A<i>β</i>1-42...
Unlike with new chemical entities, the biotransformation of therapeutic proteins (TPs) has not been routinely investigated or included in regulatory filings. Nevertheless, there is an expanding pool evidence suggesting that a more in-depth understanding could better aid discovery and development increasingly diverse modalities. For instance, such analysis TPs affords important information on molecular stability, which turn may shed light any potential impact binding affinity, potency,...
Complex nature of bioconjugates require multiple bioanalytical approaches to support PK and absorption, distribution, metabolism excretion characterization. For antibody-drug conjugate (ADC) bioanalysis both LC-MS ligand-binding assays (LBAs) are employed.A method consisting immunocapture extraction ADC from biomatrices followed by analysis light heavy chain is described. Drug antibody ratio (DAR) profiles Tras-mcVC-PF06380101 dosed at 0.3, 1 3 mg/kg in Sprague Dawley rats were obtained....
Recombinant adeno-associated virus (AAV)-mediated degeneration of sensory neurons in the dorsal root ganglia (DRG) and trigeminal (TG) has been observed non-human primates (NHPs) following intravenous (IV) intrathecal (IT) delivery. Administration recombinant AAV encoding a human protein transgene via single intra-cisterna magna (ICM) injection New Zealand white rabbits resulted histopathology changes very similar to NHPs: mononuclear cell infiltration, degeneration/necrosis neurons, nerve...
Background: The polar nucleoside drug ribavirin (RBV) combined with IFN-α is a front-line treatment for chronic hepatitis C virus infection. RBV acts as prodrug and exerts its broad antiviral activity primarily through active phosphorylated metabolite 5´-triphosphate (RTP), also possibly 5´-monophosphate (RMP). To study transport, diffusion, metabolic clearance impact on drug-metabolizing enzymes, LC–MS method needed to simultaneously quantify metabolites (RTP, 5´-diphosphate RMP). In...
15(S)-lipoxygenase-1 (15-LO-1) was present in the whole-cell homogenate of an acute human monocytic leukemia cell line (THP-1). Additionally, 15-LO-1 detected on neutral lipid droplets isolated from THP-1 foam cells. To investigate if is active droplets, we used mouse leukemic macrophage (RAW 264.7), which are stably transfected with 15-LO-1. The RAW cells were incubated acetylated low density lipoprotein to generate 15(S)-hydroxyeicosatetraenoic acid [15(S)-HETE], major metabolite...
Both plants and animals are living things made up of similar cells as well organelles, their essence life is the same. However, face more environmental stress than generate excessive reactive oxygen species (ROS), a group small molecules that can harm proteins, necessitating distinctive metabolic processes. Secondary metabolites in chemical components eliminate ROS also exhibit medicinal properties; therefore, herbal medicines often closely linked to ecological significance secondary...
Alzheimer’s disease is the most prevalent cause of dementia and associated with accumulation amyloid- β peptide (A ), particularly 42-amino acid A 1-42, in brain. 1-42 levels can be decreased by γ -secretase modulators (GSM), which are small molecules that modulate -secretase, an enzyme essential for production. BMS-869780 a potent GSM 1-40 increased 1-37 1-38, without inhibiting overall peptides or other APP processing intermediates. also did not inhibit Notch lowered brain evidence...