A5014252085- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Gut microbiota and health
- HIV-related health complications and treatments
- HIV Research and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Polysaccharides and Plant Cell Walls
- HIV/AIDS Research and Interventions
- Intensive Care Unit Cognitive Disorders
- Clostridium difficile and Clostridium perfringens research
- Polysaccharides Composition and Applications
- Prostate Cancer Treatment and Research
- Pneumonia and Respiratory Infections
- Immune Response and Inflammation
- Dialysis and Renal Disease Management
- Probiotics and Fermented Foods
- Liver Disease Diagnosis and Treatment
- Prostate Cancer Diagnosis and Treatment
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Immunodeficiency and Autoimmune Disorders
- Plant Surface Properties and Treatments
- Asthma and respiratory diseases
- Bacterial Infections and Vaccines
- Infectious Diseases and Mycology
Cape Cod Hospital
2015-2024
Genex Systems (United States)
1989-2020
Chulalongkorn University
2016
Medical University of Graz
2016
University of California, San Diego
2016
Merck (Singapore)
2016
Duke Medical Center
2007
Clinical Research Institute
2007
Duke University
2007
Duke University Hospital
2007
Measurement of (1-->3)-beta-D-Glucan (BG) has emerged as an adjunct diagnostic strategy for invasive fungal infections (IFI).Subjects at 6 clinical sites in the United States were enrolled either infection-negative subjects (n = 170) or with proven probable IFI according to European Organization Research and Treatment Cancer/Mycoses Study Group criteria 163). A central laboratory 4 performed assays. single sample was obtained per patient evaluated using assay detect serum BG derived from...
The Glucatell (1-->3)- beta-D-glucan (BG) detection assay (Associates of Cape Cod) was studied as a diagnostic adjunct for invasive fungal infections (IFIs). On the basis findings from preliminary study 30 candidemic subjects and healthy adults, serum BG level >or=60 pg/mL chosen cutoff. Testing performed with serial samples obtained 283 acute myeloid leukemia or myelodysplastic syndrome who were receiving antifungal prophylaxis. At least 1 sample positive at median 10 days before clinical...
ABSTRACT Forty-four intravenous antimicrobials were tested for the presence of (1→3)-β- d -glucan (BG). Colistin, ertapenem, cefazolin, trimethoprim-sulfamethoxazole, cefotaxime, cefepime, and ampicillin-sulbactam positive BG at reconstituted-vial concentrations but not when diluted to usual maximum plasma concentrations. False-positive assays may occur some are administered; however, this needs be confirmed.
The performance of the Fungitell assay was investigated in 100 patients with haematological malignancy undergoing chemotherapy who developed antibiotic-unresponsive neutropenic fever (AUNF). Serum β - d -glucan (BG) concentrations were significantly elevated on first day AUNF and all subsequent alternate days to 10 38 an invasive fungal infection (IFI) compared 42 remaining free such infections. mean median values BG 171.9±29.6 95.8 pg ml −1 , respectively, for IFI 64.4±17.1 32.9 only ( P...
ABSTRACT Non-culture-based diagnostic strategies are needed for diagnosing invasive candidiasis (IC). We evaluated serial serum (1→3)-β- d -glucan (BG) levels in patients the surgical trauma intensive care unit (SICU) with clinical evidence of IC. Serum samples from admitted to SICU a minimum 3 days were collected twice weekly and analyzed BG by using Fungitell kit positive cutoff ≥80 pg/ml. Diagnosis IC was done set predefined validated practice-based criteria. A total 57 consented...
Background. Improved noninvasive diagnostic tests for Pneumocystis jirovecii pneumonia (PCP) are needed. We evaluated the test characteristics of plasma (1→3)-β-D-glucan (β-glucan) HIV-related PCP among a large group patients presenting with diverse opportunistic infections (OIs).
Gastrointestinal (GI) leakage is believed to exacerbate sepsis and new, validated markers of GI barrier performance might benefit clinical decision-making. Serum (1→3)-β-D-glucan (BG) was evaluated as a potential marker. BG tested in several mouse models leakage, including dextran sulfate solution (DSS) administration, endotoxin (LPS) injection, cecal ligation puncture (CLP). titer also patients with septic shock, for comparison. With 0.75% DSS increased only after oral administration...
Gastrointestinal (GI) bacterial translocation in sepsis is well known, but the role of Lactobacillus species probiotics still controversial. We evaluated therapeutic effects rhamnosus L34 a new model oral administration pathogenic bacteria with GI leakage induced by either an antibiotic cocktail (ATB) and/or dextran sulfate sodium (DSS). ATB, DSS, and DSS plus ATB (DSS+ATB) was demonstrated fluorescein isothiocyanate (FITC)-dextran to circulation. The bacteria, Klebsiella pneumoniae or...
Background Fungal infections of the central nervous system (FICNS) are important causes morbidity and mortality among immunocompromised pediatric patients. Standard diagnostic modalities lack sensitivity for detecting therapeutically monitoring these life-threatening diseases. Current molecular methods remain investigational. (1 → 3)-β- d -glucan (BDG) is a cell wall component found in several fungal pathogens, including Candida Aspergillus spp. Detecting BDG cerebrospinal fluid (CSF) may be...
We investigated the influence of spontaneous gut leakage upon polymicrobial sepsis in a lupus model with Fc gamma receptor IIb-deficient (FcGRIIb-/-) mice aged 8 and 40 weeks, as representing asymptomatic symptomatic lupus, respectively. Spontaneous leakage, determined by (i) presence FITC-dextran, (ii) elevated serum endotoxin, (iii) (1→3)-β-D-glucan (BG), was demonstrated but not group. In parallel, detected BG without fungal infection, patients active nephritis. Gut induced dextran...
Candida albicans is the most common fungus in human intestinal microbiota but not mice. To make a murine sepsis model more closely resemble and to explore role of C. albicans, absence candidemia, bacterial sepsis, live- or heat-killed was orally administered mice at 3h prior cecal ligation puncture (CLP). A higher mortality rate CLP demonstrated with Candida-administration (live- heat-killed) CLP. Fecal presented only experiments live-Candida administration. Despite serum (1→3)-β-D-glucan...
Gut fungi may influence the course of Clostridium difficile infection either positively or negatively for host. Fungi are not prominent in mouse gut, and C. albicans, major human gastrointestinal commensal yeast, is low abundance absent mice. Bifidobacterium one probiotics that attenuate severity infection. Inflammatory synergy between albicans difficile, provide a state more closely resembles be suitable testing probiotic effects. We performed fecal mycobiota analysis administered at 1 day...
Background: CXCL13 is preferentially secreted by Follicular Helper T cells (TFH) to attract B germinal centers. Plasma levels of have been reported be elevated during chronic HIV-infection, however there limited data on such elevation early phases infection and the effect ART. Moreover, contribution disease progression systemic immune activation partially defined. Herein, we assessed relationship between plasma activation. Methods: Study samples were collected in 114 people living with HIV...
ABSTRACT 1,3-β- d -Glucan serum levels have demonstrated good diagnostic sensitivity and specificity for the diagnosis of candidiasis in adult patients, but normal children not been established. We found higher -glucan than those previously reported adults.
The role of intestinal Candida albicans in bacterial sepsis, the absence candidemia, was investigated murine models. Live C or normal saline solution (NSS) administered orally once, followed by 5 days daily oral antibiotic-mixtures (ATB). Cecal ligation and puncture (CLP) then performed to induce sepsis.Fecal detected culture only models with administration. Oral administration with/without ATB enhanced gut-pathogenic bacteria as determined microbiome analysis. Despite negative serum...
Abstract Background Despite effective antiretroviral therapy (ART), human immunodeficiency virus (HIV) infection remains associated with higher morbidity and mortality, driven, in part, by increased inflammation. Our objective was to identify associations between levels of plasma biomarkers chronic inflammation, microbial translocation, monocyte activation, occurrence non-AIDS events. Methods Participants (141 cases, 310 matched controls) were selected from a longitudinal observational...
People with HIV (PWH) taking antiretroviral therapy (ART) may experience weight gain, dyslipidemia, increased risk of non-AIDS comorbidities, and long-term alteration the gut microbiota. Both low CD4/CD8 ratio chronic inflammation have been associated changes in microbiota PWH. The antidiabetic drug metformin has shown to improve composition while decreasing diabetes polycystic ovary syndrome. Nevertheless, it remains unknown whether benefit PWH receiving ART, especially those a ratio.In...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEngineering subtilisin for reaction in dimethylformamideZiyang Zhong, Jennifer L. C. Liu, Lois M. Dinterman, Malcolm A. J. Finkelman, W. Thomas Mueller, Michele Rollence, Marc Whitlow, and Chi Huey WongCite this: Am. Chem. Soc. 1991, 113, 2, 683–684Publication Date (Print):January 1, 1991Publication History Published online1 May 2002Published inissue 1 January...
ABSTRACT The correlation of β-glucan (BG) levels with clinical outcomes in invasive candidiasis (IC) remains unknown. Patients proven IC were followed prospectively from diagnosis to outcome twice-weekly serum BG sampling. Correlation was assessed each patient. tend decrease successfully treated patients and increase treatment failures. may be useful as surrogates for evaluation IC.
The serum (1→3)-β-d-glucan assay has emerged as an important diagnostic test for invasive fungal disease. utility of this in coccidioidomycosis not been previously studied. Using a cutoff value ≥80 pg/ml, we found the sensitivity (43.9%), specificity (91.1%), positive predictive (81.8%), and negative (64.1%) to be similar those diagnosing other mycoses.
Translocation of gastrointestinal bacteria in HIV-infected individuals is associated with systemic inflammation, HIV progression, mortality, and comorbidities. are also susceptible to fungal infection colonization, but whether translocation occurs influences progression or comorbidities unknown.Serum (1→3)-β-D-glucan (BG) was measured by a Limulus Amebocyte Lysate assay (Fungitell) 132 outpatients. Selected plasma cytokines markers peripheral T-cell activation were measured. Pulmonary...
(1-3)-β-d-Glucan (BDG) from cerebrospinal fluid (CSF) is a promising marker for diagnostic and prognostic aid of central nervous system (CNS) fungal infection, but its relationship to serum values has not been studied. Herein, we detected BDG CSF at levels 2-fold lower than those in patients without evidence disease 25-fold higher noncryptococcal CNS infections. may be useful biomarker the evaluation disease.