A5005997288- Silk-based biomaterials and applications
- Viral Infectious Diseases and Gene Expression in Insects
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Neurobiology and Insect Physiology Research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Antimicrobial Peptides and Activities
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Algal biology and biofuel production
- Cancer Mechanisms and Therapy
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Studies on Chitinases and Chitosanases
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Redox biology and oxidative stress
- Autophagy in Disease and Therapy
- Reproductive biology and impacts on aquatic species
Institute of Agrobiological Sciences
2024
National Agriculture and Food Research Organization
2024
Kyushu University
2012-2022
Kumamoto University
2021-2022
Japan Society for the Promotion of Science
2009-2012
Administration of convalescent plasma or neutralizing monoclonal antibodies (mAbs) is a potent therapeutic option for coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) infection. However, SARS-CoV-2 variants with mutations in the spike protein have emerged many countries. To evaluate efficacy induced patients against emerging variants, we isolate anti-spike mAbs from two COVID-19 infected prototypic single-cell sorting immunoglobulin-G-positive (IgG
The telomere structures in Bombyx mori are thought to be maintained mainly by the transposition of specialized telomeric retroelements SART and TRAS. silkworm genome has TTAGG repeats telomerase, but this telomerase displays little or no activity. Here, we report that transcription SART1 TRAS1 is suppressed Piwi subfamily proteins BmAgo3 Siwi. was found expressed predominantly gonads early embryo, as other model organisms, BmN4 cultured cells, these formed granules were separate from nuage,...
The polymerization of proteins can create newly active and large bio-macromolecular assemblies that exhibit unique functionalities depending on the properties building block protein units in polymers. Herein, first enzymatic horseradish peroxidase (HRP) is reported. Recombinant HRPs fused with a tyrosine-tag (Y-tag) through flexible linker at N- and/or C-termini are expressed silkworm, Bombyx mori. Trametes sp. laccase (TL) used to activate tyrosine Y-tagged molecular O2 form tyrosyl-free...
Cecropins belong to the antibacterial peptides family and are induced after injection of bacteria or their cell-wall components. By silkworm cDNA microarray analysis, a novel type Cecropin gene was identified as up-regulated in early embryo, 1 day oviposition. The isolated 394 bp with 198 ORF translating 65 amino acids, encoding BmCecropin-E (BmCec-E). Using Southern hybridization genome search number BmCec-E estimated be at least two per haploid, which consisted exons, other members. mRNA...
ABSTRACT I report the complete genome sequence of an isolate nucleopolyhedrovirus from Bombyx mori , domesticated silkworm, maintained for long term at Kyushu University in Hakozaki, Fukuoka Prefecture, Japan. The is 127,783 bp long, with a G+C content 40.3%, and contains 142 open reading frames.
Polycomb group (PcG) proteins are evolutionarily conserved chromatin modifiers and act together in three multimeric complexes, repressive complex 1 (PRC1), 2 (PRC2), Pleiohomeotic (PhoRC), to repress transcription of the target genes. Here, we identified genes Bombyx mori with holocentric centromere using genome-wide expression screening based on knockdown BmSCE, BmESC, BmPHO, or BmSCM gene, which represent distinct complexes. As a result, expressions 29 were up-regulated after knocking down...
Piwi proteins are part of a superfamily Argonaute proteins, which one the core components RNA silencing pathway in many eukaryotes. thought to repress transposon expression both transcriptionally and post-transcriptionally. Recently, Drosophila melanogaster was recently reported associate with chromatin interact directly Heterochromatin Protein 1 (HP1a). However, similar interactions have not been other higher Here we show that silkworm HP1s nucleus. The silkworm, Bombyx mori, has two Ago3...
Abstract Heterochromatin protein 1s (HP1s) are nonhistone chromosomal proteins that play a direct role in the formation and maintenance of heterochromatin structure. Similarly to Caenorhabditis elegans , silkworms possess holocentric chromosomes, which diffused kinetochores extend along length each chromosome. We have isolated two silkworm HP1 homologues, BmHP1a BmHP1b. Cytological analysis showed unique localization BmHP1s during cell division, these first appear dissociate from but then...
Epigenetic modifiers and transcription factors contribute to developmentally programmed gene expression. Here, we establish a functional link between epigenetic regulation by Polycomb group (PcG) proteins transcriptional C/ebp that orchestrates the correct expression of Bombyx mori asparagine synthetase (BmASNS), involved in biosynthesis asparagine. We show cis-regulatory elements YY1-binding motifs CpG island present on BmASNS promoter are required for recruitment PcG subsequent deposition...
Abstract Small RNA ‐mediated gene silencing is a fundamental regulatory mechanism, which conserved in many organisms. Argonaute ( A go) family proteins the ‐induced complex RISC ) play crucial roles interference RNAi pathways. In silkworm B ombyx mori , four go have been identified, named as go1, go2, go3 and S iwi. go2 participates double‐stranded dsRNA )‐induced whereas iwi are involved P iwi‐interacting piRNA pathway. However, there no experimental evidence concerning go1 BmAgo1...
RNAi induction by the injection of long dsRNA into hemocoel has been tried to archive efficient gene knockdown in silkworm larvae or pupae, and severas reports successfully applying have brought out. However, effect is often insufficient improvement efficiency desirable. On other hand, metabolic turnover organ uptake injected insects not sufficiently clarified so far. In this study, we report that larval hemolymph binding activity. We also show molecular entity activity lipophorin, a major...
Long hairpin dsRNA transcribed from chromosomal DNA can induce RNA interference in Bombyx mori cells, although its gene silencing efficiency is lower than that of exogenously introduced double-stranded RNAs (dsRNAs). To solve this problem, we monitored the nuclear cytoplasmic translocation and analyzed processing into mature small interfering (siRNA). Northern blot analysis revealed dsRNAs were spliced transported cytoplasm, but not effectively diced siRNAs. Interestingly, RNAi with...
We investigated the gene structure and predicted amino acid sequence of antioxidant enzyme 2-Cys peroxiredoxin (2-Cys Prx) in raphidophyte Chattonella marina, which is a harmful algal bloom (HAB) species. The open reading frame Prx was 585 bp long encoded protein consisting 195 acids. putative contained two cysteine residues located at 49th 170th positions from N-terminal methionine residue. also possessed characteristic motifs, F (FFYPLDFTFVCPTEI) EVCP. position relative to several others...
Recombinant protein production can create artificial proteins with desired functions by introducing genetic modifications to the target proteins. Horseradish peroxidase (HRP) has been used extensively as a reporter enzyme in biotechnological applications; however, recombinant of HRP not very successful, hampering utilization modifications. A fusion comprising an antibody binding and will be ideal bio-probe for high-quality HRP-based diagnostic systems. HRP-protein A/G (HRP-pAG) is designed...