A5091558850- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Immunotherapy and Immune Responses
- Nuclear Structure and Function
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Muscle Physiology and Disorders
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- Immune responses and vaccinations
- Congenital limb and hand anomalies
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related molecular mechanisms research
- Plant and animal studies
- Adipose Tissue and Metabolism
- Atherosclerosis and Cardiovascular Diseases
- Antibiotic Resistance in Bacteria
- RNA regulation and disease
- Prosthetics and Rehabilitation Robotics
- Bone fractures and treatments
University of Utah
2019-2024
Huntsman Cancer Institute
2019-2024
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2018-2022
National Institutes of Health
2018
Children's National
2016
George Washington University
2013-2016
Military Medical Academy
2013
University of Belgrade
2011
ABSTRACT Dedicated stem cells ensure postnatal growth, repair and homeostasis of skeletal muscle. Following injury, muscle (MuSCs) exit from quiescence divide to reconstitute the cell pool give rise progenitors. The transcriptomes pooled MuSCs have provided a rich source information for describing genetic programs distinct static states; however, bulk microarray RNA sequencing provide only averaged gene expression profiles, blurring heterogeneity developmental dynamics asynchronous MuSC...
Mutations in lamin A/C and emerin change myogenic cell fate by disrupting heterochromatin tethering to the nuclear envelope.
In response to various types of infection, naïve CD4
The genetic context of the blaNDM-1 gene in genome Pseudomonas aeruginosa MMA83 was investigated. Sequencing cosmid selected for revealed presence two copies P. a unique environment. Additionally, mating assays, DNA-DNA hybridization, and an S1 nuclease assay strongly suggest that is chromosome borne.
The transition between pluripotent and tissue-specific states is a key aspect of development. Understanding the pathways driving these transitions will facilitate engineering properly differentiated cells for experimental therapeutic uses. Here, we showed that during mesoderm differentiation, transcription factor Oct1 activated developmental lineage–appropriate genes were silent in cells. Using mouse embryonic stem (ESCs) with an inducible knockout Oct1, deficiency resulted poor induction...
The molecular mechanisms leading to the establishment of immunological memory are inadequately understood, limiting development effective vaccines and durable antitumor immune therapies. Here, we show that ectopic OCA-B expression is sufficient improve antiviral recall responses, while having minimal effects on primary effector responses. At peak viral response, short-lived T cell populations expanded but increased Gadd45b Socs2 expression, precursor cells Bcl2 , Il7r, Tcf7 a per-cell basis....
The transcriptional coregulator OCA-B promotes expression of T cell target genes in cases repeated antigen exposure, a necessary feature autoimmunity. We hypothesized that cell–specific deletion and pharmacologic inhibition would protect mice from autoimmune diabetes. developed an Ocab conditional allele backcrossed it onto diabetes-prone NOD/ShiLtJ strain background. loss protected spontaneous disease. Protection was associated with large reductions islet CD8+ receptor specificities...
Embryonic stem cells (ESCs) can adopt lineage-specific gene-expression programs by stepwise exposure to defined factors, resulting in the generation of functional cell types. Bulk and single-cell-based assays were employed catalog gene expression, histone modifications, chromatin conformation, accessibility transitions ESC populations individual acquiring a presomitic mesoderm fate undergoing further specification toward myogenic neurogenic lineages. These identified cis-regulatory regions...
We evaluated the degree of reproductive isolation between laboratory populations seed beetle (Acanthoscelides obtectus) selected to reproduce early (E) or late (L) in life, where different levels sexual activity and discrimination have been detected. found a significant level behavioral among within E selection regime which beetles showed enhanced early-life fitness traits low activity. In contrast, substantially higher an indiscriminate mating system inhibited rather than promoted...
Thymic presentation of self-antigens is critical for establishing a functional yet self-tolerant T-cell population. Hybrid peptides formed through transpeptidation within pancreatic β-cell lysosomes have been proposed as new class autoantigens in type 1 diabetes (T1D). While the production hybrid thymus has not explored, due to nature their generation, it thought be highly unlikely. Therefore, peptide-reactive thymocytes may preferentially escape thymic selection and contribute significantly...
Background During terminal differentiation of cells, there is typically a transition the nuclear envelope from Lamin B protein to A/C proteins. This commensurate with exit cell cycle, and maintenance transcriptional programs associated terminally differentiated cells. Dominant missense mutations in cause broad spectrum human genetic disorders, where specific point are defects organs or tissues. We have previously presented model disrupt developmentally appropriate interactions between...
ABSTRACT The transition between pluripotent and tissue-specific states is a key aspect of development. Understanding the pathways driving these transitions will facilitate engineering properly differentiated cells for experimental therapeutic uses. Here, we showed that during mesoderm differentiation, transcription factor Oct1 activated developmental lineage-appropriate genes were silent in cells. Using mouse embryonic stem (ESCs) with an inducible knockout Oct1, deficiency resulted poor...
Abstract The molecular mechanisms leading to the establishment of immunological memory are inadequately understood, limiting development effective vaccines and durable anti-tumor immune therapies. Here we show that ectopic OCA-B expression is sufficient improve antiviral recall responses, while having minimal effects on primary effector responses. At peak viral response short lived T cell populations expanded but increased Gadd45b Socs2 expression, precursor cells Bcl2 , Il7r Tcf7 a per-cell...
Abstract The establishment of durable immunological memory remains a poorly understood aspect lymphocyte biology and limiting factor in vaccine development. Using T cell-conditional OCA-B (Pou2af1) knockout model mouse viral pathogen, we show that expression the transcription cofactor within cells is necessary for proper CD4+ cell formation. We also ectopic sufficient to drive towards fate while having no effect on primary antiviral effector response. Bulk single-cell gene profiling...
Abstract The transcriptional coregulator OCA-B promotes expression of T cell target genes in cases repeated antigen exposure, a necessary feature autoimmunity. We hypothesized that cell-specific deletion and pharmacologic inhibition would protect mice from autoimmune diabetes. developed an Ocab conditional allele backcrossed it onto diabetes-prone NOD/ShiLtJ strain background. loss protected spontaneous disease. Protection was associated with large reductions islet CD8 + receptor...
Abstract Treatments for autoimmunity aim to inhibit autoreactivity while preserving normal immune function. The transcriptional coregulator OCA-B (gene symbol Pou2af1) is induced in stimulated naïve CD4+ T cells, where docks with transcription factor Oct1 regulate genes such as Il2 and Ifng. only their targets cases of persistent antigen exposure, a common feature autoimmunity. We hypothesized that cell-specific deletion would protect mice from autoimmune diabetes, pharmacologic inhibition...
SUMMARY ESCs can adopt lineage-specific gene expression programs by stepwise exposure to defined factors, resulting in the generation of functional cell types. Bulk and single cell-based assays were employed catalogue expression, histone modifications, chromatin conformation, accessibility transitions ESC populations individual cells acquiring a presomitic mesoderm fate undergoing further lineage specification. These identified cis -regulatory regions transcription factors presiding...
<p> </p> <p>Thymic presentation of self-antigens is critical for establishing a functional yet self-tolerant T cell population. Hybrid peptides formed through transpeptidation within pancreatic beta lysosomes have been proposed as new class autoantigens in Type 1 Diabetes (T1D). While the production hybrid thymus has not explored, due to nature their generation, it thought be highly unlikely. Therefore, peptide-reactive thymocytes may preferentially escape thymic selection...
<p> </p> <p>Thymic presentation of self-antigens is critical for establishing a functional yet self-tolerant T cell population. Hybrid peptides formed through transpeptidation within pancreatic beta lysosomes have been proposed as new class autoantigens in Type 1 Diabetes (T1D). While the production hybrid thymus has not explored, due to nature their generation, it thought be highly unlikely. Therefore, peptide-reactive thymocytes may preferentially escape thymic selection...
Abstract The differentiation of CD4+ T cell subsets in response to infection has been studied extensively, however the epigenetic programs that regulate these processes remain poorly understood. Active demethylation by tet methylcytosine dioxygenases CpG dinucleotides within DNA is a key component programing promotes lineage specific gene expression and contributes cellular function. Here we report Tet2 acts restrict follicular helper (Tfh) cells responding viral infection. Using an adoptive...