A5054702350- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- interferon and immune responses
- CRISPR and Genetic Engineering
- Chronic Lymphocytic Leukemia Research
- Cancer Genomics and Diagnostics
- Paleontology and Evolutionary Biology
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Aquaculture disease management and microbiota
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Viral-associated cancers and disorders
- Ichthyology and Marine Biology
- Circadian rhythm and melatonin
- Telomeres, Telomerase, and Senescence
- Sirtuins and Resveratrol in Medicine
- Cytomegalovirus and herpesvirus research
- Epigenetics and DNA Methylation
University of Colorado Anschutz Medical Campus
2020-2022
University of Colorado Denver
2022
University of Rochester
2012-2021
Shanghai Ocean University
2010-2019
University of Rochester Medical Center
2014
University of California, San Francisco
2012
Genomic instability is a hallmark of aging tissues. may arise from the inefficient or aberrant function DNA double-stranded break (DSB) repair. DSBs are repaired by homologous recombination (HR) and nonhomologous end joining (NHEJ). HR precise pathway, whereas NHEJ frequently leads to deletions insertions at repair site. Here, we used normal human fibroblasts with chromosomally integrated reporter cassette examine changes in efficiency as cells progress replicative senescence. We show that...
The naked mole-rat (Heterocephalus glaber) is a subterranean eusocial rodent with markedly long lifespan and resistance to tumorigenesis. Multiple data implicate modulation of protein translation in longevity. Here we report that 28S ribosomal RNA (rRNA) the processed into two smaller fragments unequal size. breakpoints are located rRNA divergent region 6 excise fragment 263 nt. excised unique does not show homology other genomic regions. Because this hidden break site could alter ribosome...
Clonal hematopoiesis of indeterminate potential is prevalent in elderly individuals and associated with increased risks all-cause mortality cardiovascular disease. However, mouse models to study the dynamics clonal its consequences on system under homeostatic conditions are lacking. We developed a model using adoptive transfer unfractionated ten-eleven translocation 2-mutant (Tet2-mutant) bone marrow cells into nonirradiated mice. Consistent age-related observed humans, these mice displayed...
Most human somatic cells do not divide indefinitely but enter a terminal growth arrest termed replicative senescence. Replicatively senescent are generally believed to in G1 or G0 stage of the cell cycle. While doing cycle analysis on three different lines normal fibroblasts we observed that 36-60% replicatively had 4N DNA content. Only up 5% more than one nucleus ruling out possibility population were G1-arrested bi-nucleated cells. Furthermore, it is unlikely tetraploids, because actively...
Significance The naked mole rat is a longest lived and cancer-resistant rodent. Tumor resistance in the mediated by signals from extracellular matrix component hyaluronan triggering induction of INK4 (inhibitors cyclin dependent kinase 4) locus expression. human mouse encodes three critical tumor-suppressor proteins, p15 INK4b , ARF (alternate reading frame), p16 INK4a which are among most frequently mutated cancer. Furthermore, implicated aging senescence. Here, we show that an additional...
Hematopoietic stem cells (HSCs) are capable of entering the cell cycle to replenish blood system in response inflammatory cues; however, excessive proliferation chronic inflammation can lead either HSC attrition or expansion. The mechanism(s) that limit and expansion triggered by signals poorly defined. Here, we show long-term HSCs (HSCLT) rapidly repress protein synthesis genes following treatment with proinflammatory cytokine interleukin (IL)-1. This gene program is associated activation...
Naked mole rat (NMR) is a valuable model for aging and cancer research due to its exceptional longevity resistance. We observed that the reprogramming efficiency of NMR fibroblasts in response OSKM was drastically lower than mouse fibroblasts. Expression SV40 LargeT antigen (LT) dramatically improved Inactivation Rb alone, but not p53, sufficient improve efficiency, suggesting chromatin may be refractory reprogramming. Analysis global histone landscape revealed had higher levels repressive...
Abstract Background Low-dose Computed Tomography (CT) is used for the detection of pulmonary nodules, but ambiguous risk evaluation causes overdiagnosis. Here, we explored significance DNA methylation 7 genes including TAC1 , CDO1 HOXA9 ZFP42 SOX17 RASSF1A and SHOX2 in blood cfDNA samples distinguishing lung cancer from benign nodules healthy individuals. Method A total 149 patients [72 mass 77 ground-glass (GGNs)], 5 48 individuals were tested analyzed this study. The lasso-logistic...
Summary Whether errors in protein synthesis play a role aging has been subject of intense debate. It suggested that rare mistakes young organisms may result the machinery, eventually leading to an increasing cascade as age. Studies followed generally failed identify dramatic increase translation with aging. However, whether fidelity plays remained open question. To address this issue, we examined relationship between and maximum lifespan across 17 rodent species diverse lifespans. measure...
Abstract Background Evidence has demonstrated that monitoring of the variable, diversity, and joining gene segments (VDJ) rearrangement immunoglobulin (Ig) genes in circulating tumor DNA (ctDNA) is value predicting outcomes diffuse large B cell lymphoma (DLBCL). In this study, we investigated role VDJ proportion ctDNA for DLBCL progression. Methods Patients diagnosed with newly were included study. The sequences IgH detected using next-generation sequencing (NGS) formalin-fixed...
Abstract Purposes Evidence has demonstrated that monitoring of the variable, diversity, and joining gene segments (VDJ) rearrangement immunoglobulin (Ig) in circulating tumor DNA (ctDNA) is highly valuable predicting prognosis patients with diffuse large B cell lymphoma (DLBCL). In this study, we investigated role both Ig heavy chain (IGH) kappa light (IGK) rearrangements detected ctDNA samples DLBCL progression. Methods Next-generation sequencing (NGS) was used to identify dominant V(D)J...
Cervical cancer is preceded by low-grade squamous intraepithelial lesions (LSIL) and high-grade (HSIL). Human papillomavirus (HPV) test a sensitive method for cervical screening, but it less specific compared with cytological examination, leading to overtreatment reduced patient compliance. Therefore, new detection methods that can improve the accuracy of screening are needed. In present study, exfoliated cell samples were collected from 228 Chinese individuals, including 114 healthy control...
Epstein-Barr virus (EBV)-positive nodal T-cell and NK-cell lymphoma is a rare neoplasm of cytotoxic or lineage. Here, we report 26 cases affecting 14 men 12 women with median age 52 years. All patients presented disease involving multiple lymph nodes, 20 22 (91%) fully staged had advanced Ann Arbor stage disease. Spleen, liver, bone marrow were involved in 70%, 50%, 52% cases, respectively. These dismal prognosis survival 30 days. Histologically, nodes replaced by diffuse pattern. Lymphoma...
Abstract Calorie restriction (CR) improves health, reduces cancer incidence and extends lifespan in multiple organisms including mice. CR was shown to enhance base excision repair nucleotide pathways of DNA repair, however, whether double-strand breaks has not been examined vivo system. Here we utilize non-homologous end joining (NHEJ) reporter mice show that short-term strongly enhances by NHEJ, which is associated with elevated levels DNA-PK SIRT6.
Flatfish is famous for the asymmetric transformation during metamorphosis. The molecular mechanism behind development has been speculated over a century and still not well understood. To date, none of metamorphosis-related genes identified in flatfish. As first step to screen gene, we constructed whole-body cDNA library miRNA this study 1051 unique ESTs, 23 miRNAs, 4 snoRNAs premetamorphosing prometamorphosing Paralichthys olivaceus . 1005 ESTs were novel, suggesting that there was special...
Abstract Osteoarthritis (OA) is the most prevalent disabling disease, affecting quality of life and contributing to morbidity, particularly during aging. Current treatments for OA are limited palliation: pain management surgery end‐stage disease. Innovative approaches animal models needed develop curative OA. Here, we investigated naked mole‐rat (NMR) as a potential model resistance. NMR small rodent with maximum lifespan over 30 years, resistant wide range age‐related diseases. tissues...
Many flatfish species, such as Scophthalmus maximus, Paralichthys olivaceus, Solea senegalensis, are extensively cultured worldwide. In flatfishes, eye migration varies among different species and even within the same species. The genetic mechanism underlying is still unclear, it key to improve metamorphosis in aquaculture understand evolution. this study, high frequency of location variants an artificial S. senegalensis population allowed us investigate preference during migration;...