Significance Statement Myeloid-derived suppressor cells are innate suppressors that play an immunoregulatory role in autoimmunity, transplantation, and antitumor immunity; however, their effects on renal ischemia-reperfusion injury remain unclear. The authors showed granulocyte colony-stimulating factor (G-CSF) increased infiltration of myeloid-derived after injury. When given before ischemia-reperfusion, G-CSF subsequently attenuated acute tissue injury, apoptosis, inflammation; when...

The role of hyperuricemia in disease progression autosomal dominant polycystic kidney (ADPKD) has not been defined well. We investigated the association serum uric acid (sUA) with renal function and effect hypouricemic treatment on rate decline. This is a single-center, retrospective, observational cohort study. A total 365 patients ADPKD who had estimated glomerular filtration (eGFR) ≥ 15 mL/min/1.73 m2 were followed up for > 1 year included our analysis. Hyperuricemia was by sUA level 7.0...

Abstract Background Cold ischemia‐reperfusion injury (IRI) is an unavoidable complication of kidney transplantation. We investigated the role regulatory T cells (Treg) in cold IRI and whether interleukin (IL)‐2/anti‐IL‐2 antibody complex (IL‐2C) can ameliorate IRI. Methods developed a mouse model using transplantation analyzed IL‐2C impact on acute, subacute chronic phases. Results Treg transfer attenuated IRI, while depletion aggravated Next, administration prior to mitigated acute renal...

Abstract Renal ischemia/reperfusion injury (IRI) is a common form of acute kidney injury. The basic mechanism underlying renal IRI inflammation, where oxidative stress plays an important role. Although bilirubin exhibits potent reactive oxygen species (ROS)‐scavenging properties, its clinical application hindered by problems associated with solubility, stability, and toxicity. In this study, BX‐001N, synthetic polyethylene glycol‐conjugated 3α nanoparticle developed assessed renoprotective...

Macrophages play important roles in xenograft rejection. Here, we investigated whether overexpression of human CD200 or CD47 porcine endothelial cells (PEC) can suppress macrophages activation xenogeneic immune responses.PECs and were incubated together, harvested, analyzed for vitro macrophage phagocytic cytotoxicity activity, cytokine release. Next, PECs injected into renal subcapsular space humanized mice. On day 10 posttransplantation, survival perigraft inflammatory cell infiltrations...

Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common hereditary diseases that frequently result in renal failure. In this cross-sectional observational cohort study, we evaluated urinary angiotensinogen (AGT) as a potential biomarker to assess function ADPKD. Urinary AGT was measured 233 ADPKD patients and its association with estimated glomerular filtration rate (eGFR) height-adjusted total volume (htTKV) were evaluated. The localization other renin-angiotensin...

Granulocyte colony-stimulating factor (G-CSF) can induce regulatory T cells (Tregs) as well myeloid-derived suppressor (MDSCs). Despite the immune modulatory effects of G-CSF, results G-CSF treatment in systemic lupus erythematosus are still controversial. We therefore investigated whether ameliorate nephritis and studied underlying mechanisms. NZB/W F1 female mice were treated with or phosphate-buffered saline for 5 consecutive days every week from 24 weeks age, analyzed at 36 age....

Regulatory B cells are a newly discovered cell subset that suppresses immune responses. Recent studies found both anti-CD45RB and anti-Tim-1 treatments regulate responses by inducing regulatory cells; however, the role of these in renal ischemia-reperfusion injury (IRI) is unknown.Using mouse models, including T cell-deficient (RAG1 knockout TCRα knockout) mice (μMT) mice, we investigated effects on IRI mechanisms underlying effects.Adoptive transfer before or after attenuated IRI....

Plasmapheresis in combination with immunoglobulin and rituximab is often used to induce accommodation ABO-incompatible (ABOi) living-donor transplantation; however, this regimen cannot be applied cases of ABOi deceased-donor transplantation. Here, we investigated whether an anti-complement component 5 (C5) antibody-based can heart transplantation.Both IgM IgG anti-blood type A antibodies were induced wild-type mice by sensitization using human blood antigen. Heterotopic transplantation was...

Tissue-engineered vascular microphysiological platform is applicable for evaluating immune modulation of xenograft rejection.

Human B-1 cells have been proposed to be CD20+CD27+CD43+CD1c- B found in the umbilical cord and adult peripheral blood, but their regulatory mechanisms not well elucidated. Previously, we reported that mouse CD49dhigh CD4+ T could enhance secretion of natural antibodies by cells. In this study, aimed investigate presence helper functions human equivalents murine Here, showed peritoneal cavity (PEC), spleen, blood can production IgM As revealed mouse, were more abundant PEC a higher tendency...

The major obstacle to successful ABO blood group-incompatible kidney transplantation (ABOi KT) is antibody-mediated rejection (AMR). This study aimed investigate transcriptional profiles through RNA sequencing and develop a minimally invasive diagnostic tool for discrimination between accommodation early acute AMR in ABOi KT. Twenty-eight KT patients were selected: 18 with 10 at the 10th day posttransplant protocol biopsy. Complete transcriptomes of their peripheral analyzed by sequencing....

Foxp3 stability of vitamin C-treated induced-regulatory T cells (V-iTregs) is superior to that conventional iTregs (C-iTregs). However, the role V-iTregs in allograft rejection under C-deficient conditions, such as those seen humans, remains unclear. We aimed elucidate C treatment on generation and maintenance from gulo knockout (Gulo-KO) mice well wild type (WT) mice, vitro vivo suppressive effects heart either Gulo-KO or WT recipient mice. Conversion efficiency was similar between C- both...

Studies on B-cell subtypes and V(D)J gene usage of receptors in kidney transplants are scarce. This study aimed to investigate segment ABO-incompatible (ABOi) transplant (KT) patients compared that ABO-compatible (ABOc) KT patients. We selected 16 ABOi with accommodation (ABOiA), 6 ABOc stable (ABOcS), biopsy-proven acute antibody-mediated rejection (ABOiR) at day 10, whose graft tissue samples had been stored the biorepository between 2010 2014. Complete transcriptomes tissues were...

Abstract Antibody-mediated rejection (ABMR) is one of the main obstacles to successful transplantation, including ABO blood group-incompatible (ABOi) transplantation. Recently, chimeric antigen receptor regulatory T cells (CAR Tregs) were developed improve specificity, viability, and suppressive activity Tregs. C4d deposition a marker ABMR also found in most ABOi allograft tissues. Based on these findings, we anti-C4d CAR Tregs suppress allografts. Anti-C4d prepared by retroviral...

Background Studies on B-cell subtypes and V(D)J gene usage of receptors in kidney transplants are scarce. This study aimed to investigate segment ABO-incompatible (ABOi) transplant (KT) patients compared that ABO-compatible (ABOc) KT patients. Methods We selected 16 ABOi with accommodation (ABOiA), 6 ABOc stable (ABOcS), biopsy-proven acute antibody-mediated rejection (ABOiR) at day 10, whose graft tissue samples had been stored the biorepository between 2010 2014. Complete transcriptomes...

Introduction: Antibody-mediated rejection (ABMR) is the main hurdle in ABO blood group-incompatible (ABOi) transplantation. C4d deposition a marker of ABMR and also found most ABOi allograft tissues as result accommodation. Previously, we reported that anti-C4d chimeric antigen receptor (CAR) regulatory T cells (Tregs) suppressed allografts. Here, aimed to improve efficacy CAR Tregs by modifying structure increasing dose Tregs. Methods: Intracellular costimulatory domains Treg consisted...

Introduction: Pigs have been proposed as a promising source of the global shortage donor organ. However, there are several problems immune rejection caused by differences between species. Several genetic combinations reported to try solve this problem; however, additional research is needed on transgenic pigs. In study, we developed multi-transgenic Yucatan miniature pigs using gene manipulation and somatic cell nuclear transfer (SCNT) eliminate GGTA1, CMAH, iGb3s, β4GalNT2 (quadruple...

Introduction: CD200 suppresses xenogeneic immune responses of macrophages similarly as CD47. Moreover, can suppress other cells, such T cells. Previous studies demonstrated that overexpression human (hCD200) in pig endothelial cells successfully suppressed vascular xenograft rejection to greater extent than CD47 humanized mice. Based on these data, we produced hCD200-transgenic (TG) pigs. This study aimed explore beneficial effects hCD200-TG pigs nonhuman primate kidney xenotransplantation....

Introduction: Neutrophil extracellular traps (NETs) formation, or NETosis, is a type of programmed cell death that involves the release traps, which plays an important role in immune response against pathogens. Still, it can also contribute to development various pathological conditions. There was some evidence NETs play significant rejection allotransplantation; were released lung transplantation, and disruption increased graft survival (1). In study pig-to-baboon xenotransplants,...

Joon Young Jang, Ji-Jing Yan, Dongsuk Kim, Joohyun Shim, Kimyung Choi, Kyo Won Lee, Jae Berm Park, Beom Seok Sung-Joo Jaeseok Yang. Korean J Transplant 2023;37:256. https://doi.org/10.4285/ATW2023.F-8500

Suchen Kumar Yadav, Jong Cheol Jeong, Sunghoon Hurh, Boem Seok Kim, Joon Young Jang, Ji Jin Yan, Sungjoo Curie Ahn, Jaeseok Yang. Korean J Transplant 2023;37:185. https://doi.org/10.4285/ATW2023.F-7708