A5027149454- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Cytokine Signaling Pathways and Interactions
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Multiple Myeloma Research and Treatments
- Immune Cell Function and Interaction
- Whipple's Disease and Interleukins
- interferon and immune responses
- Neonatal Health and Biochemistry
- Transgenic Plants and Applications
- Inflammasome and immune disorders
- Peroxisome Proliferator-Activated Receptors
- Immune Response and Inflammation
- Infant Nutrition and Health
- Heme Oxygenase-1 and Carbon Monoxide
- Cannabis and Cannabinoid Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Mass Spectrometry Techniques and Applications
- NF-κB Signaling Pathways
- Diabetes and associated disorders
- Pharmacological Effects of Natural Compounds
- Apelin-related biomedical research
- Protease and Inhibitor Mechanisms
- Circadian rhythm and melatonin
Korea Research Institute of Bioscience and Biotechnology
2003-2020
Korea University of Science and Technology
2016-2020
Konkuk University
2010-2016
Hallym University
2016
Konkuk University Medical Center
2010-2013
Chungnam National University
2006
Cell Biotech (South Korea)
2003
IL-33/IL-1F11 is a new member of the IL-1 family ligand and provokes T helper-type immune responses. IL-33 ST2 receptor accessory protein (IL-1RAcP) that triggers nuclear factor-κ light chain enhancer activated B cells (NF-κB) MAPK signaling. We discovered novel short splice variant was termed spIL-33. The spIL-33 lacks exon 3 containing proposed caspase-1 cleavage site. isolated cDNA from Huh7 human hepatocarcinoma cell line expressed recombinant in Escherichia coli. pro-IL-33 were not...
IL-1 family ligand does not possess a typical hydrophobic signal peptide and needs processing enzyme for maturation. The maturation process of IL-33 (IL-1F11), new member the ligand, remains unclear. Precursor affinity column isolates neutrophil proteinase 3 (PR3) from human urinary proteins. PR3 is known ligand-processing IL-1β (IL-1F2) IL-18 (IL-1F4), including other inflammatory cytokines. We investigated in precursor because we isolated by using column. converted inactive mouse proteins...
Abstract Proteolysis targeting chimeras (PROTACs) are an emerging strategy for promoting targeted protein degradation by inducing the proximity between proteins and E3 ubiquitin ligases. Although successful of numerous PROTACs has been demonstrated, elements that determine degradability PROTAC-targeted have not yet explored. In this study, we developed von Hippel-Lindau-Cereblon (VHL-CRBN) heterodimerizing induce CRBN, but VHL. A quantitative proteomic analysis further revealed VHL-CRBN...
Abstract Atopic dermatitis (AD) is a common chronic inflammatory skin disease with growing prevalence worldwide. Topical corticosteroids and non‐steroidal calcineurin inhibitors (CNIs) are used as first‐line therapies for AD, but various side effects limit their long‐term use. Here, the first synthetic bilirubin(IIIα)‐derived nanoparticle reported, designated BRNP(IIIα), shows that it exhibits robust antioxidative immunomodulatory effectively reduces clinical symptoms of AD upon topical...
Abstract α 1 -Antitrypsin (AAT) is a member of the serine proteinase inhibitor family that impedes enzymatic activity proteinases, including human neutrophil elastase, cathepsin G and 3. Here, we expressed recombinant AAT by fusing intact gene to constant region IgG1 generate soluble AAT-Fc protein. The protein was produced in Chinese hamster ovary (CHO) cells purified using mini-protein A affinity chromatography. Recombinant tested for antiinflammatory function sufficiently suppressed tumor...
Abstract Renal ischemia/reperfusion injury (IRI) is a common form of acute kidney injury. The basic mechanism underlying renal IRI inflammation, where oxidative stress plays an important role. Although bilirubin exhibits potent reactive oxygen species (ROS)‐scavenging properties, its clinical application hindered by problems associated with solubility, stability, and toxicity. In this study, BX‐001N, synthetic polyethylene glycol‐conjugated 3α nanoparticle developed assessed renoprotective...
Replacement therapy is the most common treatment for reduction of bleeding and control episodic in individuals with hemophilia. Despite proven effectiveness factor replacement therapy, repeated intravenous administration a heavy burden to
The pro-inflammatory cytokines tumor necrosis factor-α (TNFα) and interleukin (IL)-1β are crucial mediators involved in chronic inflammatory diseases. Inflammatory signal pathways regulate cytokine expression-mediated by p38 mitogen activated protein kinase (p38MAPK). Therefore, considerable attention has been given to p38MAPK as a target molecule for the development of novel anti-inflammatory therapeutics. BIRB 796, one inhibitor, is candidate therapeutic drug In this study, we investigated...
In order to produce a human lactoferrin (hLf) protein in cultured plant cells, we developed Korean ginseng (Panax ginseng) cell line using an oxidative stress-inducible peroxidase (SWPA2) promoter and characterized the production of cells. A construct containing targeting signal peptide from tobacco endoplasmic reticulum fused cDNA under control SWPA2 was engineered. Transgenic lines that produced recombinant hLf were successfully generated confirmed by PCR Southern blot analyses. Western...
It has been reported that fatty acid binding proteins (FABPs) do not act only as intracellular mediators of lipid responses but also have extracellular functions. This study aimed to investigate whether liver type (L)-FABP a biological activity and determined serum L-FABP levels in patients with end-stage renal disease (ESRD). We isolated complementary deoxyribonucleic (cDNA) from the Huh7 human hepatocarcinoma cell line expressed recombinant protein Escherichia coli. A549 lung carcinoma...
Cytokines are essential coordinators of defensive immune responses for resolving the invasion pathogens such as bacteria, virus, and fungi. However, dysregulated cytokines main cause various autoinflammatory disorders rheumatoid arthritis, inflammatory bowel disease, psoriasis. Interleukin-32 (IL-32) is a recently described cytokine characterized proinflammatory cytokine. IL-32 stimulates monocytes macrophages to induce important (IL-1β, IL-6, TNFα) chemokines (IL-8 MIP-2) by activating...
Sung Ah Kim, Seung-Hyun Jo, Jin Hwa Cho, Min Yeong Yu, Ho-Chul Shin, Jung-Ae Goo Park, Byoung Chul Sunhong and Jeong-Hoon Kim. Mol. Cells 2020;43:935-44. https://doi.org/10.14348/molcells.2020.0122
Although it has been established that diabetes increases susceptibility to infections, the role of insulin (INS) in immune response is unknown. Here, we investigated immunological function INS. Proinsulin dimer (pINSd) was a potent stimulus induced inflammatory cytokines, but mature INS unable induce an response. An affinity-purified rabbit polyclonal antibody raised against IL-1α recognized and pINS failed detect IL-1β. Analysis sequence revealed existence INS/IL-1α motif C-peptide pINS....
Enhancer of zeste 2 polycomb repressive complex subunit (EZH2), the catalytic (PRC2), regulates genes involved in cell lineage and differentiation through methylating lysine 27 on histone H3 (H3K27me3). Recurrent gain-of-function mutations EZH2 have been identified various cancer types, particular, diffuse large B-cell lymphoma (DLBCL), large-scale genome-wide association studies depletion or pharmacological inhibition has shown to exert an antiproliferative effect cells, both vitro vivo. In...
Lipid profiling in nine bacterial species has been accomplished by laser desorption ionization mass spectrometry (LDI-MS) using amorphous silicon (a-Si) thin film with 100 nm thickness. ions could be generated LDI on a-Si regardless of ion acquisition modes because a thermal property to govern laser-induced surface heating. In comparative study lipid Bacillus lichemiformis LDI-MS and matrix-assisted desorption/ionization (MALDI-MS), shows higher efficiency lipopeptide detection than...