A5060830854- Cytokine Signaling Pathways and Interactions
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Eosinophilic Esophagitis
- Psoriasis: Treatment and Pathogenesis
- Autophagy in Disease and Therapy
- interferon and immune responses
- Pharmacological Effects of Natural Compounds
- Inflammasome and immune disorders
- Protease and Inhibitor Mechanisms
- Whipple's Disease and Interleukins
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- Metabolism, Diabetes, and Cancer
- Heat shock proteins research
- Bioactive natural compounds
- Blood Coagulation and Thrombosis Mechanisms
- Signaling Pathways in Disease
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- ATP Synthase and ATPases Research
- Peroxisome Proliferator-Activated Receptors
Catholic University of Daegu
2021-2024
Daegu Catholic University Medical Center
2023
National Cancer Institute
2016-2021
Konkuk University
2008-2020
Center for Cancer Research
2016-2020
National Institutes of Health
2020
Cancer Genetics (United States)
2016
Cardiovascular Research Center
2015
Brigham and Women's Hospital
2015
Konkuk University Medical Center
2010-2013
The rationale of α1-antitrypsin (AAT) augmentation therapy to treat progressive emphysema in AAT-deficient patients is based on inhibition neutrophil elastase; however, the benefit this treatment remains unclear. Here we show that clinical grade AAT (with elastase inhibitory activity) and a recombinant form (rAAT) without anti-elastase activity reduces lung inflammatory responses LPS elastase-deficient mice. WT mice treated with either native or rAAT exhibited significant reductions...
Similar to IL-1α and IL-33, IL-1 family member IL-37b translocates the nucleus is associated with suppression of innate adaptive immunity. Here we demonstrate an extracellular function IL-37 precursor a processed form. Recombinant reduced LPS-induced IL-6 by 50% (P < 0.001) in highly inflammatory human blood-derived M1 differentiated macrophages derived from selective subjects but not M2 macrophages. In contrast, neutralizing monoclonal anti-IL-37 increased IL-6, TNFα IL-1β 0.01). The was...
Significance IL-37 exerts broad inhibitory properties on the innate inflammatory and acquired immune responses. We mutated caspase-1 site in show that processing is required for maturation of intracellular precursor its translocation to nucleus. Because nuclear suppression LPS-induced IL-6, data define a unique consequence inhibition, is, reversal anti-inflammatory activities endogenous IL-37. In addition, neutralizing antibodies reverse IL-6 transgenic mice, supporting role extracellular...
Summary Cytokines are crucial in host defence against pathogens such as bacteria, viruses, fungi and parasites. A newly described cytokine, interleukin‐32 (IL‐32), induces various proinflammatory cytokines (tumour necrosis factor‐α, IL‐1β, IL‐6) chemokines both human mouse cells through the nuclear factor‐κB p38 mitogen‐activated protein kinase inflammatory signal pathway. The IL‐32 primarily acts on monocytic rather than T cells. In an attempt to isolate soluble receptor, we used...
Significance The association of hypertension and inflammation is clear, but mechanisms for this human finding remain elusive. experiments in article demonstrate that a highly local intramyocardial signaling pathway through ST2, the receptor proinflammatory cytokine interleukin 33 (IL-33), regulates heart’s response to pressure overload. By generating using new conditional deletion mice, we identified endothelial cells as major source systemic circulating IL-33. Our study reveals cell...
Atractylodes macrocephala Koidz (AMK) is a traditional herbal medicine used for thousands of years in East Asia to improve variety illnesses and conditions, including cancers. This study explored the effect AMK extract on apoptosis tumor-grafted mice using AGS human gastric adenocarcinoma cells. We investigated compounds, target genes, associated diseases Traditional Chinese Medical Systems Pharmacy (TCMSP) database platform. Cell viability assay, cell cycle mitochondrial depolarization...
Inflammatory cytokines mediate inflammatory bowel diseases (IBDs) and cytokine blocking therapies often ameliorate the disease severity. IL-32 affects inflammation by increasing production of IL-1, TNFα, several chemokines. Here, we investigated role in intestinal generating a transgenic (TG) mouse expressing human IL-32γ (IL-32γ TG). Although TG mice are healthy, constitutive serum colonic tissue levels TNFα elevated. Compared with wild-type (WT) mice, exhibited modestly exacerbated acute...
IL-33/IL-1F11 is a new member of the IL-1 family ligand and provokes T helper-type immune responses. IL-33 ST2 receptor accessory protein (IL-1RAcP) that triggers nuclear factor-κ light chain enhancer activated B cells (NF-κB) MAPK signaling. We discovered novel short splice variant was termed spIL-33. The spIL-33 lacks exon 3 containing proposed caspase-1 cleavage site. isolated cDNA from Huh7 human hepatocarcinoma cell line expressed recombinant in Escherichia coli. pro-IL-33 were not...
IL-1 family ligand does not possess a typical hydrophobic signal peptide and needs processing enzyme for maturation. The maturation process of IL-33 (IL-1F11), new member the ligand, remains unclear. Precursor affinity column isolates neutrophil proteinase 3 (PR3) from human urinary proteins. PR3 is known ligand-processing IL-1β (IL-1F2) IL-18 (IL-1F4), including other inflammatory cytokines. We investigated in precursor because we isolated by using column. converted inactive mouse proteins...
Recent studies found that short-chain fatty acids (SCFAs), which are produced through bacterial fermentation in the gastrointestinal tract, have oncoprotective effects against cervical cancer. The most common SCFAs well known include acetic acid, butyric and propionic among acid (PA) has been reported to induce apoptosis HeLa cells. However, mechanism suppress cell viability remain poorly understood. Our study aims provide a more detailed look into of PA Flow cytometry analysis revealed...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) is a positive-sense single-stranded RNA (+ssRNA) that causes disease 2019 (COVID-19). The viral genome encodes twelve genes for replication and infection. third open reading frame the spike (S) gene glycoprotein interacting with specific cell surface receptor - angiotensin converting enzyme (ACE2) on host membrane. Most recent studies identified single point mutation in S gene. A leading to an amino acid substitution at codon 614...
Since interleukin (IL)-18 is a proinflammatory cytokine, mice lacking IL-18 or its ligand-binding receptor (IL-18R) should exhibit decreased cytokine and chemokine production. Indeed, production of IL-1alpha, IL-6, MIP-1alpha was reduced in knock-out (ko) mouse embryonic fibroblast (MEF)-like cells. Unexpectedly, we observed paradoxical 10-fold increase IL-1beta-induced IL-6 MEF cells from deficient the IL-18R alpha-chain (IL-18Ralpha) compared with wild type MEF. Similar increases were for...
Abstract α 1 -Antitrypsin (AAT) is a member of the serine proteinase inhibitor family that impedes enzymatic activity proteinases, including human neutrophil elastase, cathepsin G and 3. Here, we expressed recombinant AAT by fusing intact gene to constant region IgG1 generate soluble AAT-Fc protein. The protein was produced in Chinese hamster ovary (CHO) cells purified using mini-protein A affinity chromatography. Recombinant tested for antiinflammatory function sufficiently suppressed tumor...
It is well known that activated microglia produce nitric oxide (NO), which has an important role in the pathophysiology of several neurodegenerative diseases such as Alzheimer's disease. In course searching for novel therapeutic agents from medicinal plants against neuroinflammatory diseases, methanolic extract Tetrapanax papyriferus was found to have significant NO inhibitory activity lipopolysaccharide (LPS)-stimulated BV2 cells. Nine oleanane-type triterpenes, including two new compounds,...
Interleukin-32 (IL-32) induces a variety of proinflammatory cytokines and chemokines. The IL-32 transcript was reported originally in activated T cells; subsequently, it demonstrated to be abundantly expressed epithelial endothelial cells upon stimulation with inflammatory cytokines. is regulated robustly by other major cytokines, thereby suggesting that crucial inflammation immune responses. Recently, an IL-32alpha-affinity column employed order isolate binding protein, neutrophil...
The pro-inflammatory cytokines tumor necrosis factor-α (TNFα) and interleukin (IL)-1β are crucial mediators involved in chronic inflammatory diseases. Inflammatory signal pathways regulate cytokine expression-mediated by p38 mitogen activated protein kinase (p38MAPK). Therefore, considerable attention has been given to p38MAPK as a target molecule for the development of novel anti-inflammatory therapeutics. BIRB 796, one inhibitor, is candidate therapeutic drug In this study, we investigated...
To evaluate the role of IL-32 in granulomatosis with polyangiitis (GPA) patients and relationship between disease activity, as PR3 has ability to bind activate IL-32, which been described a novel cytokine that induces inflammatory cytokines.We investigated level PR3, TNF-α IL-6 GPA by using ELISA. Northern blot was used analyse mRNA leucocytes patients. The intracellular colocalization examined IF staining.We observed levels were increased significantly when compared normal individuals each...
Oxygen sensing is crucial for adaptation to variable habitats and physiological conditions. Low oxygen tension, or hypoxia, a common feature of solid tumors, hypoxic tumors are often more aggressive resistant therapy. Here we show that, in cultured mammalian cells, hypoxia suppressed lysosomal acidification/activation receptor tyrosine kinase (RTK) degradation. Hypoxia down-regulated mTORc1, reducing its ability activate transcription factor EB (TFEB), master regulator V-ATPase, the proton...