A5026388689- Genomics and Phylogenetic Studies
- Biofuel production and bioconversion
- Immune Cell Function and Interaction
- Microbial Metabolic Engineering and Bioproduction
- Microbial Community Ecology and Physiology
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- Enzyme Production and Characterization
- Probiotics and Fermented Foods
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- CAR-T cell therapy research
- Microbial Fuel Cells and Bioremediation
- Wastewater Treatment and Nitrogen Removal
- Methane Hydrates and Related Phenomena
- Animal Virus Infections Studies
- Microbial Metabolites in Food Biotechnology
- T-cell and B-cell Immunology
- Cancer Research and Treatments
- Biosensors and Analytical Detection
- Composite Structure Analysis and Optimization
- Anaerobic Digestion and Biogas Production
- Polyamine Metabolism and Applications
- Water Treatment and Disinfection
Korea University of Science and Technology
2018-2025
Korea Research Institute of Standards and Science
2014-2025
University of Science and Technology
2020-2025
Chungnam National University
2025
Korea International Cooperation Agency
2024
Korea Research Institute of Chemical Technology
2018-2023
Chung-Ang University
2021
Seoul National University
2002-2018
University of Southern California
2012-2018
Korea Institute of Science and Technology
2011-2014
16S rRNA gene sequences have been widely used for the identification of prokaryotes. However, flood non-type strains and lack a peer-reviewed database type made routine isolates difficult labour-intensive. In present study, we generated containing all prokaryotic strains. addition, web-based tool, named EzTaxon, analysis was constructed to achieve based on pairwise nucleotide similarity values phylogenetic inference methods. The system developed provides users with similarity-based search,...
A strictly anaerobic, Gram-stain-positive, non-spore-forming, rod-shaped bacterial strain, designated BS-1T, was isolated from an anaerobic digestion reactor during a study of bacteria utilizing galactitol as the carbon source. Its cells were 0.3-0.5 μm × 2-4 μm, and they grew at 35-45 °C pH 6.0-8.0. Strain BS-1T produced H2, CO2, ethanol, acetic acid, butyric acid caproic metabolic end products fermentation. Phylogenetic analysis, based on 16S rRNA gene sequence, showed that strain...
Coronavirus disease 2019 (COVID-19) is a newly emerging human infectious caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2, also previously known as 2019-nCoV). Within 8 months of the outbreak, more than 10,000,000 cases COVID-19 have been confirmed worldwide. Since human-to-human transmission occurs easily and rate infection rapidly increasing, sensitive early diagnosis essential to prevent global outbreak. Recently, World Health Organization (WHO) announced various primer-probe...
Abstract Coronavirus disease 2019 (COVID-19) is newly emerging human infectious diseases, which caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2, also previously known as 2019-nCoV). Within two months of the outbreak, more than 80,000 cases COVID-19 have been confirmed worldwide. Since to transmission occurred easily and infection rapidly increasing, sensitive early diagnosis essential prevent global outbreak. Recently, World Health Organization (WHO) announced various primer probe...
Strain MHT, a strictly anaerobic, Gram-stain-negative, non-spore-forming, spherical coccus or coccoid-shaped microorganism, was isolated from cow rumen during screen for hexanoic acid-producing bacteria. The microorganism grew at 30–40 °C and pH 5.5–7.5 exhibited production of various short- medium-chain carboxylic acids (acetic acid, butyric pentanoic isobutyric isovaleric heptanoic acid octanoic acid), as well H2 CO2 biogas. Phylogenetic analysis based on 16S rRNA gene sequencing...
The aim of this study was to investigate the potential anti-cancer effects probiotic cell-free supernatant (CFS) treatment using Lactobacillusfermentum for colorectal cancer (CRC) in 3D culture systems. Cell viability assessed MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) assays, whereas apoptosis monitored through RT-qPCR analysis Bax, Bak, Noxa, and Bid mRNA expressions addition flow cytometry Lactobacillus (LCFS) treatment. Our...
The World Health Organization (WHO) has declared the coronavirus disease 2019 (COVID-19) as an international health emergency. Current diagnostic tests are based on reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method, which is gold standard test that involves amplification of viral RNA. However, RT-qPCR assay limitations in terms sensitivity and quantification. In this study, we tested both qPCR droplet digital PCR (ddPCR) to detect low amounts cycle threshold (CT)...
Abstract Background Currently, the most promising microorganism used for bio-production of butyric acid is Clostridium tyrobutyricum ATCC 25755 T ; however, it unable to use sucrose as a sole carbon source. Consequently, newly isolated strain, Bacillu s sp. SGP1, that was found produce levansucrase enzyme, which hydrolyzes into fructose and glucose, in co-culture with this permitting C. ferment acid. Results B. SGP1 alone did not show any production main metabolite produced lactic This...
Abstract Coronavirus disease 2019 (COVID-19) is a new emerging human infectious caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2, also previously known as 2019-nCoV), originated in Wuhan seafood and animal market, China. Since December 2019, more than 69,000 cases of COVID-19 have been confirmed China quickly spreads to other counties. Currently, researchers put their best efforts identify effective drugs for COVID-19. The neutralizing antibody, which binds viral capsid manner that...
Microbial community analysis based on the 16S rRNA-gene is used to investigate both beneficial and harmful microorganisms in various fields environments. Recently, next-generation sequencing (NGS) technology has enabled rapid accurate microbial analysis. Despite these advantages of NGS metagenomics study, sample transport, storage conditions, amplification, library preparation kits, sequencing, bioinformatics procedures can bias results. In this eight mock communities were pooled from...
A strictly anaerobic, Gram-negative, non-spore-forming bacterium, designated GH1(T), was isolated from the rumen of Korean native cattle (HanWoo). Cells were straight to slightly curved rods (2.0-4.5 µm long) and motile by peritrichous flagella. The isolate grew at 30-45 °C (optimum 40 °C), pH 5.5-6.5 6.0) with up 3.5% (w/v) NaCl. Strain GH1(T) produced acid d-glucose, d-ribose d-xylose, butyric being major end product. genomic DNA G+C content 54.6 mol%. Based on comparative 16S rRNA gene...
Recent studies found that short-chain fatty acids (SCFAs), which are produced through bacterial fermentation in the gastrointestinal tract, have oncoprotective effects against cervical cancer. The most common SCFAs well known include acetic acid, butyric and propionic among acid (PA) has been reported to induce apoptosis HeLa cells. However, mechanism suppress cell viability remain poorly understood. Our study aims provide a more detailed look into of PA Flow cytometry analysis revealed...
Despite a high degree of conservation, subtle but important differences exist between the CD1d antigen presentation pathways humans and mice. These may account for minimal success natural killer T (NKT) cell-based antitumor therapies in human clinical trials, which contrast strongly with powerful effects conventional mouse models. To develop an accurate model vivo (hCD1d) presentation, we have generated hCD1d knock-in (hCD1d-KI) mouse. In these mice, is expressed native tissue distribution...
A facultatively anaerobic, moderately halophilic, Gram-negative, filamentous bacterium, designated JC2469T, was isolated from tidal flat sediment in Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to phylum Bacteroidetes and its closest taxonomic relative Cytophaga fermentans NCIMB 2218T (89.6 % sequence similarity). Cells appeared as filaments during exponential growth, but became fragmented rods at stationary phase. Spherical cells were also...
Herpes simplex virus 1 (HSV-1) causes one of the most prevalent herpesviral infections in humans and is leading etiological agent viral encephalitis eye infections. Our understanding how HSV-1 interacts with host at cellular organismal levels still limited. We others previously reported that, upon infection, rapidly efficiently downregulates CD1d cell surface expression suppresses function NKT cells, a group innate T cells critical immunoregulatory function. The protein kinase US3 plays...
Background Vibrio parahaemolyticus is a major disease-causing species of that pathogenic to both farmed shrimp and humans. With the increasing spread antibiotic-resistant V. strains, bacteriophages (or phages) are considered potential agents for biocontrol as an alternative antibiotics. In this study, bacteriophage capable lysing , named Pv27, was isolated, characterized, evaluated its control infections natural therapy. Methods Phage Pv27 isolated using double-layer agar technique...
Abstract Background The 2022 dengue outbreak in Karachi, Pakistan, posed a severe threat to the region, yet no study has investigated origins of causal strain. Methods This aimed trace and transmission route virus serotype 1 (DENV-1) strain responsible for epidemic through genome sequencing phylogenetic analyses. We successfully sequenced 135 complete DENV-1 genomes from clinical samples using long-read amplicon sequencing. Results genotype III circulating Pakistan likely originated...