A5103010910- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- Renal and related cancers
- Ovarian function and disorders
- Ovarian cancer diagnosis and treatment
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Health and Technologies
- Endometrial and Cervical Cancer Treatments
- Prenatal Screening and Diagnostics
- Gynecological conditions and treatments
- Pluripotent Stem Cells Research
- Cervical Cancer and HPV Research
- Cancer Genomics and Diagnostics
- Multiple Sclerosis Research Studies
- Gestational Trophoblastic Disease Studies
- Intraperitoneal and Appendiceal Malignancies
- Maternal and Perinatal Health Interventions
- Pregnancy-related medical research
- Molecular Biology Techniques and Applications
- Maternal and fetal healthcare
- Particle Accelerators and Free-Electron Lasers
- Pregnancy and preeclampsia studies
- Underwater Vehicles and Communication Systems
Akita University
2016-2025
Akita University Hospital
2011-2024
Tokyo Metropolitan University
2022
Akita Industrial Technology Center
2021
National Institute of Technology, Kochi College
2018
Health Sciences University of Hokkaido
2015
Tohoku University
2003-2013
Iwate Prefectural Central Hospital
2010
Institute for Materials Research, Tohoku University
2004-2008
Institute of Materials Research of the Slovak Academy of Sciences
2004-2008
In mammalian follicles, oocytes are arrested at the diplotene stage of prophase I until meiotic resumption following LH surge. Recently, C-type natriuretic peptide (CNP), encoded by precursor type C (NPPC) was found to suppress mouse oocyte maturation promoting cyclic guanosine 5'-monophospate (cGMP) production in cumulus cells. However, regulation NPPC/CNP expression during pre-ovulatory period and their surge have not been investigated.Based on genome-wide analysis DNA microarray data sets...
Periodontitis is a multifactorial disease in which bacterial, lifestyle, and genetic factors are involved. Although previous association studies identified several susceptibility genes for periodontitis European populations, there little information Asian populations. Here, we conducted genome-wide study replication consisting of 2,760 Japanese patients 15,158 controls. single-nucleotide polymorphisms that surpassed stringent significance threshold ( P < 5 × 10 −8 ) were not identified,...
Abstract During human embryonic development, early cleavage-stage embryos are more susceptible to errors. Studies have shown that many problems occur during the first mitosis, such as direct cleavage, chromosome segregation errors, and multinucleation. However, mechanisms whereby these errors mitosis in remain unknown. To clarify this aspect, present study, we image discarded living two-pronuclear stage zygotes using fluorescent labeling confocal microscopy without microinjection of DNA or...
Lynch syndrome (LS) is an autosomal-dominant inherited disorder mainly caused by a germline mutation in the DNA mismatch repair (MMR) genes (MLH1, MSH2, MSH6, and PMS2) associated with increased risk for various cancers, particularly colorectal cancer endometrial (EC). Women LS account 2% to 6% of EC patients; it clinically important identify such individuals predicting and/or preventing additional LS-associated cancers. PMS2 (PMS2-LS) rarest contribution etiology among 4 MMR mutations, its...
This prospective study evaluated the accuracy of non-invasive preimplantation genetic testing for aneuploidy (niPGT-A) using cell-free DNA in spent culture medium, as well that (PGT-A) trophectoderm (TE) biopsy after culturing beyond implantation. Twenty frozen blastocysts donated by 12 patients who underwent IVF at our institution were investigated. Of these, 10 on day 5 and 6. Spent medium TE cells collected from each blastocyst thawing, embryos cultured vitro up to days. The outgrowths...
Does a new system—the chip-sensing embryo respiration monitoring system (CERMs)—enable evaluation of viability for potential application in clinical IVF setting? The enabled the oxygen consumption rate spheroids, bovine embryos and frozen-thawed human to be measured, this corresponded developmental embryos. To date, no reliable clinically suitable objective methods are available, which circumvent differences inter-observer subjective view. Existing systems such as scanning electrochemical...
High cesarean section (CS) rates in middle and high-income countries are partly attributable to provider factors, such as staffing patterns fear of litigation. However, the relationship between CS healthcare resources community is poorly understood. Official data on has been particularly limited Japan. In this study, we examined nationwide statistics evaluated association with local for perinatal care.We used accumulated registered Japan National Database health insurance claims 2013...
Jasplakinolide (JAS), which induces microfilament polymerization and stabilization, inhibits microfilament-mediated events in murine oocyte maturation fertilization a fashion unlike the effects of cytochalasin B (CCB) latranculin A (LAT A). JAS prevents egg polar body emission at much lower concentration than either CCB or LAT A. Microfilament bundles were detected on entire cortex after exposure. Conversely, patterns did not change exposure to CCB, few microfilaments observed Eggs that...
The antitumor effects of anti-PD-1 antibody against mismatch repair deficiency (MMR-D)-associated cancers have been reported. MMR-D is found in approximately 20%-30% endometrial carcinomas (ECs) and frequently occurs due to MLH1 promoter hypermethylation (MLH1-PHM). ECs with MLH1-PHM are classified according the molecular screening Lynch syndrome (LS), but few detailed reports available. purpose this study was clarify clinical features EC MLH1-PHM.Immunohistochemistry MMR proteins (MLH1,...
BACKGROUND: Centrosomal function and oocyte activation ability of human sperm from a case globozoospermia was assayed by heterologous ICSI into bovine oocytes. METHODS: Microtubules chromatin configuration in oocytes were examined immunofluorescence after with two fertile donors globozoospermic man. RESULTS: The microtubule array the centrosome, 'sperm aster' male pronucleus observed oocytes, following round-headed rate aster formation pronuclear injected donor 57.9 92.5% respectively;...
Parvovirus B19 is a small DNA virus. Infection with parvovirus during pregnancy may cause serious complications in the fetus, including hydrops fetalis and fetal death. The purpose of present study to clarify clinical manifestations outcomes infection pregnancy. This prospective enrolled 478 women suspected infections between 1999 2004. One hundred cases (21%) were detected pregnant who had been exposed B19. Serological was confirmed by measurement B19-specific IgM IgG sera. Forty-nine...
To show whether molecular motor dynein on a microtubule track, myosin Va, recruiter VIIa-Rab27a/b interacting protein (MyRIP), and vesicle receptor Rab27b an F-actin track were present during human monkey spermiogenesis involving intramanchette transport (IMT).Spermiogenic cells obtained from three men with obstructive azoospermia normal adult cynomolgus (Macaca fascicularis). Immunocytochemical detection reverse transcription-polymerase chain reaction (RT-PCR) analysis of the proteins...
To examine the effect that human papillomavirus (HPV) vaccination has had in Akita Prefecture, by comparing cervical cytology results from women aged 20-24 years with or without HPV vaccination.We examined 2425 subjects who underwent cancer screening under jurisdiction of Foundation for Healthcare between January 2014 and October 2016. We compared prevalence cytologic abnormalities among vaccination.The rate atypical squamous cells undetermined significance (ASC-US) worse was 0.242% (1/413)...
The aim of this study was to investigate neutrophil elastase (NE) in amniotic fluid as a potential marker for predicting pregnancy continuation.
Abstract Aim Due to the lack of national perinatal registries, population‐wide descriptive statistics on cesarean deliveries in Japan are unknown. We aim describe for overall and multiple pregnancies using National Database health insurance claims. Methods calculated prefectural delivery rates 2014. described maternal morbidities (e.g., blood transfusion) place type institutions providing prenatal care. Results The were 18.6% 82.7% women with pregnancies. Prefectural varied from 12.5% 24.2%...