A5007936128- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Autoimmune Neurological Disorders and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Long-Term Effects of COVID-19
- Genetics and Neurodevelopmental Disorders
- SARS-CoV-2 and COVID-19 Research
- Fibroblast Growth Factor Research
- Peripheral Neuropathies and Disorders
- Neuroscience and Neuropharmacology Research
- Multiple Sclerosis Research Studies
- Chromatin Remodeling and Cancer
- Herpesvirus Infections and Treatments
- RNA regulation and disease
- Polyomavirus and related diseases
- COVID-19 Clinical Research Studies
- Genetic Neurodegenerative Diseases
- Inflammatory Myopathies and Dermatomyositis
- Sinusitis and nasal conditions
- Mitochondrial Function and Pathology
- Alzheimer's disease research and treatments
- Skin Protection and Aging
- Thyroid Cancer Diagnosis and Treatment
- Erythrocyte Function and Pathophysiology
- Heparin-Induced Thrombocytopenia and Thrombosis
University of California, San Francisco
2019-2025
Emory University
2024
University Medical Center HCMC
2024
University of California, San Diego
2023
Santa Clara University
2023
Stanford University
2023
Broad Center
2023
University of New Haven
2023
University of California, Irvine
2022
Baylor College of Medicine
2022
Individuals with coronavirus disease 2019 (COVID-19) frequently develop neurological symptoms, but the biological underpinnings of these phenomena are unknown. Through single-cell RNA sequencing (scRNA-seq) and cytokine analyses cerebrospinal fluid (CSF) blood from individuals COVID-19 we find compartmentalized, CNS-specific T cell activation B responses. All affected had CSF anti-severe acute respiratory syndrome 2 (SARS-CoV-2) antibodies whose target epitopes diverged serum antibodies. In...
Neuropsychiatric manifestations of COVID-19 have been reported in the pediatric population.To determine whether anti-SARS-CoV-2 and autoreactive antibodies are present cerebrospinal fluid (CSF) patients with subacute neuropsychiatric dysfunction.This case series includes 3 recent SARS-CoV-2 infection as confirmed by reverse transcriptase-polymerase chain reaction or IgG serology exposure history who were hospitalized at University California, San Francisco Benioff Children's Hospital for...
Vitamin B12 is critical for hematopoiesis and myelination. Deficiency can cause neurologic deficits including loss of coordination cognitive decline. However, diagnosis relies on measurement vitamin in the blood, which may not accurately reflect concentration brain. Using programmable phage display, we identified an autoantibody targeting transcobalamin receptor (CD320) a patient with progressive tremor, ataxia, scanning speech. Anti-CD320 impaired cellular uptake cobalamin (B12) vitro by...
Objective Co‐occurring anti‐tripartite motif‐containing protein 9 and 67 autoantibodies (TRIM9/67‐IgG) have been reported in only a very few cases of paraneoplastic cerebellar syndrome. The value these biomarkers the most sensitive methods TRIM9/67‐IgG detection are not known. Methods We performed retrospective, multicenter study to evaluate cerebrospinal fluid serum candidate by tissue‐based immunofluorescence, peptide phage display immunoprecipitation sequencing, overexpression cell‐based...
Abstract One third of COVID-19 patients develop significant neurological symptoms, yet SARS-CoV-2 is rarely detected in central nervous system (CNS) tissue, suggesting a potential role for parainfectious processes, including neuroimmune responses. We therefore examined immune parameters cerebrospinal fluid (CSF) and blood samples from cohort with complications. found divergent immunological responses the CNS compartment, increased levels IL-12 IL-12-associated innate adaptive cell...
Mutations in Sonic Hedgehog (SHH) signaling pathway genes, for example, Suppressor of Fused (SUFU), drive granule neuron precursors (GNP) to form medulloblastomas (MB SHH ). However, how different molecular lesions the Shh transformation is frequently unclear, and SUFU mutations cerebellum seem distinct. In this study, we show that fibroblast growth factor 5 (FGF5) integral many infantile MB cases FGF5 expression uniquely upregulated tumors. Similarly, mice lacking (Sufu-cKO) ectopically...
Neuronal progenitors in the developing forebrain undergo dynamic competence states to ensure timely generation of specific excitatory and inhibitory neuronal subtypes from distinct neurogenic niches dorsal ventral forebrain, respectively. Here we show evidence progenitor plasticity when Sonic hedgehog (SHH) signaling is left unmodulated embryonic neocortex mammalian forebrain. We found that, at early stages corticogenesis, loss Suppressor Fused (Sufu), a potent inhibitor SHH signaling,...
Neuroinvasive infection is the most common cause of meningoencephalitis in people living with human immunodeficiency virus (HIV), but autoimmune etiologies have been reported. We present case a 51-year-old man HIV steroid-responsive whose comprehensive pathogen testing was non-diagnostic. Subsequent tissue-based immunofluorescence acute-phase cerebrospinal fluid revealed anti-neural antibodies localizing to axon initial segment (AIS), node Ranvier (NoR), and subpial space. Phage display...
Many researchers have studied differences in conditions of ethnic skin using biophysical measurements. However, few studies to date focused on the antioxidative capacity skin.We measured two parameters oxidative stress stratum corneum, catalase activity and protein carbonylation corneum (SCCP), groups, Japanese French subjects, characterize susceptibility stress. We also several physiological at three different sites, sun-exposed sites (cheek dorsal aspect hand) a sun-protected site (inner...
To identify the autoantigen in 2 individuals with possible seronegative paraneoplastic neuropathy.Serum and CSF were screened by tissue-based assay panned for candidate autoantibodies phage display immunoprecipitation sequencing (PhIP-Seq). The antigen was validated immunostaining knockout tissue HEK 293T cell-based assay.Case 1 presented gait instability, distal lower extremity numbness, paresthesias after a recent diagnosis of serous uterine fallopian carcinoma. Case had remote history...
Abstract Ataxia with anti-regulator of G-protein signaling 8 autoantibodies (RGS8-Abs) is an autoimmune disease recently described in four patients. The present study aimed to identify other patients RGS8-Abs, describe their clinical features, including the link between RGS8-related cerebellar ataxia (ACA) and cancer. Patients RGS8-Abs were identified retrospectively biological collections French Reference Center for Paraneoplastic Neurological Syndrome University California San Francisco...
NMDA receptor mediated autoimmune encephalitis (NMDAR-AE) frequently results in persistent sensory-motor deficits, especially children, yet the underlying mechanisms remain unclear. This study investigated long- term effects of exposure to a patient-derived GluN1-specific monoclonal antibody (mAb) during critical developmental period (from postnatal day 3 12) mice. We observed long-lasting deficits characteristic NMDAR-AE, along with permanent changes callosal axons within primary...
Epileptic seizures induce aberrant neurogenesis from resident neural stem cells (NSCs) in the dentate gyrus of adult mouse hippocampus, which has been implicated depletion NSC pool and impairment hippocampal function. However, mechanisms regulating after remain unknown. Here, we demonstrate that Sonic hedgehog (Shh) mossy is a major source Shh signaling activity seizures, by contribute to seizure-induced maintenance pool. Deletion attenuates neurogenesis. Moreover, absence cells, NSCs are...
Abstract Vitamin B12 is critical for hematopoiesis and myelination. 1 Deficiency can cause neurologic deficits including loss of coordination, spasticity, cognitive decline. 2,3,4 However, diagnosis relies on vitamin measurement in the blood which may not accurately reflect levels brain. Here, we discovered an autoimmune deficiency restricted to central nervous system (CNS), termed (ABCD). Using programmable phage display, identified autoantibody targeting transcobalamin receptor (CD320) a...
The development of autoimmune antibody panels has improved the diagnosis paraneoplastic neurological disorders (PNDs) brain and spinal cord. Here, we present a case woman with history breast cancer who presented subacute sensory ataxia that progressed over 18 months. Her examination diagnostic studies were consistent myelopathy. Metabolic, infectious, testing non-diagnostic. However, she responded to empirical immunosuppression, prompting further workup for an etiology. An unbiased...
Mutations in Sonic Hedgehog (SHH) signaling pathway genes, e.g. Suppressor of Fused (SUFU), drive granule neuron precursors (GNP) to form medulloblastomas (MB SHH ). However, how different molecular lesions the Shh transformation is frequently unclear, and SUFU mutations cerebellum seem distinct. In this study, we show that fibroblast growth factor 5 (FGF5) integral for many infantile MB cases FGF5 expression uniquely upregulated tumors. Similarly, mice lacking (Sufu-cKO), ectopically...
Mutations in Sonic Hedgehog (SHH) signaling pathway genes, for example, Suppressor of Fused (SUFU), drive granule neuron precursors (GNP) to form medulloblastomas (MB SHH ). However, how different molecular lesions the Shh transformation is frequently unclear, and SUFU mutations cerebellum seem distinct. In this study, we show that fibroblast growth factor 5 (FGF5) integral many infantile MB cases FGF5 expression uniquely upregulated tumors. Similarly, mice lacking (Sufu-cKO) ectopically...