A5101797920- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Soybean genetics and cultivation
- Vector-borne infectious diseases
- Hepatitis Viruses Studies and Epidemiology
- Microbial infections and disease research
- Molecular Biology Techniques and Applications
- Pneumonia and Respiratory Infections
- T-cell and Retrovirus Studies
- Cervical Cancer and HPV Research
- Dengue and Mosquito Control Research
- Streptococcal Infections and Treatments
- Lipid metabolism and biosynthesis
- Immunodeficiency and Autoimmune Disorders
- Bacterial Infections and Vaccines
- Plant biochemistry and biosynthesis
- interferon and immune responses
- Identification and Quantification in Food
- Coconut Research and Applications
- Genomics and Phylogenetic Studies
- Hepatitis B Virus Studies
- Immune Cell Function and Interaction
- Bacteriophages and microbial interactions
- Circular RNAs in diseases
Kunming Children's Hospital
2017-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2022
Kunming Medical University
2017-2020
Institute of Crop Sciences
2020
Chinese Academy of Agricultural Sciences
2020
Yangtze University
2020
Yunnan Center for Disease Control And Prevention
2017
Peking Union Medical College Hospital
2017
Antibody dependent enhancement (ADE) of dengue virus (DENV) infection is identified as the main risk factor severe Dengue diseases. Through opsonization by subneutralizing or non-neutralizing antibodies, DENV suppresses innate cell immunity to facilitate viral replication. However, it largely unknown whether suppression type-I IFN necessary for a successful ADE infection. Here, we report that both and DENV-ADE induce an early ISG (NOS2) expression through RLR-MAVS signalling axis independent...
Acute bacterial meningitis is still considered one of the most dangerous infectious diseases in children. To investigate prevalence and antibiotic resistance profiles cerebrospinal fluid (CSF) pathogens children with acute Southwest China, CSF samples from 179 patients (3 days to 12 years old) positive culture results were collected 2012 2015. Isolated identified using Vitek-32 system. Gram stain used guide subcultures susceptibility testing. The antimicrobial isolates was determined disc...
HPV-16 long control region (LCR) has been shown to be the most variable of genome and may play important roles in viral persistence development cervical cancer. This study aimed assess risk LCR variants for cancer women Southwest China. 2146 scrapings volunteer outpatients 74 tissues were screened.14 entire LCRs from asymptomatic carriers 34 patients successfully amplified sequenced align others described. 58 different point mutations detected 54 nucleotide sites LCR. G7193T G7521A variants,...
A dengue outbreak abruptly occurred at the border of China, Myanmar, and Laos in June 2017. By November 3rd2017, 1184 infected individuals were confirmed as NS1-positive Xishuangbanna, a city located border. To verify causative agent, complete genome information was obtained through PCR sequencing based on viral RNAs extracted from patient samples. Phylogenetic trees constructed by maximum likelihood method (MEGA 6.0). Nucleotide amino acid substitutions analyzed BioEdit, followed RNA...
Abstract A total of 1067 serum samples were collected from febrile patients in Xishuangbanna, Yunnan, 2015. Of these, 852 cases confirmed to be dengue NS1-positive. 76 structural protein genes sequenced through RT-PCR based on the viral RNAs extracted samples. Phylogenetic analysis revealed that all strains classified as cosmopolitan genotype DENV-2. After comparing with DENV-2SS, 173 base substitutions found sequences, resulting 43 nonsynonymous mutations, which 22 mutations existed among...
Variations in HPV LCR/E6/E7 have been shown to be associated with the viral persistence and cervical cancer development. So far, there are few reports about polymorphisms of HPV-58 sequences Southwest China. This study aims characterize gene women China, assess effects variations on immune recognition E6 E7 antigens. Twelve isolates were amplified sequenced. A neighbor-joining phylogenetic tree was constructed by MEGA 7.0, followed secondary structure prediction related proteins using...
Xishuangbanna, a border area of China, Burma and Laos, had its first major DENV-1 outbreak in 2017. This study aims to explore the genetic characterization, potential source evolution viruses outbreak.The structural protein C/prM/E genes isolated from local residents or Burmese travelers were sequenced followed by mutation, phylogenetic, homologous recombination, molecular clock demographic reconstruction analysis.Phylogenetic analysis revealed that all strains classified as three cluster...
Dengue virus (DV) has occasionally emerged at epidemic levels in Yunnan, China. Vaccine development is limited by antibody-dependent enhancement and a lack of good animal models. Thus, the study investigated cross infection based on maternal immunity BALB/c mice assessed risk DV2-D13113 DV3-YNWS2 strains. DV replicated within organs infant mice, even causing death. Particularly, DV3-infected were higher severe disease if their mothers infected with DV2. Although adults pups survived DV2/DV3...
Dengue virus infection mainly causes dengue hemorrhagic fever (DHF) and/or shock syndrome (DSS). However, ADE (antibody-dependent enhancement) is one of the main pathogenic factors, and its mechanism has not been fully elucidated. Recently, with development high-throughput sequencing, an increased number RNAs have confirmed to play a vital regulatory role in process infection. there lack research on ADE. In this study, we used RNA-Seq detect differentially expressed (DE RNAs) profiles...
The live attenuated hepatitis A virus vaccine (HA-L) is in routine use the Chinese national immunization program (NIP). major disadvantages of HA-L include that theoretically, it may be possible for mutation shifts and secondary infections viral strain. aim this study was to explore variation strain after vaccination with HA-L. total 1297 fecal samples (including 470 18 36-month-old age group, 527 3 16-year-old 300 16 years older group) were collected study, rate (HAV) positivity 11.36%...
Seed-coat color has changed from black to yellow during natural and artificial selection of cultivated soybean wild soybean, it is also an important morphological marker. Therefore, discovering genes related the seed-coat will play a very role in breeding evolutionary study. Different colors caused by deposition various anthocyanin pigments. Although pigmentation been well dissected at molecular level several plant species, controlling variation remain be unknown. Genes were discussed this...
Abstract In the context of recent arbovirus epidemics, dengue fever is becoming a greater concern around world. August 2017, Xishuangbanna, which border city China, Burma and Laos, had its first major outbreak. A total 156 serum samples from febrile patients were collected; 97 DENV positive screened out, viral RNAs successfully directly extracted, including 77 cases China 20 Myanmar. Phylogenetic analysis revealed that all strains classified as DENV-1. There are eight epidemic Myanmar 74...