A5012939200- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Listeria monocytogenes in Food Safety
- Microbial Metabolites in Food Biotechnology
- Food composition and properties
- Bacteriophages and microbial interactions
- Cassava research and cyanide
- Plant Pathogens and Fungal Diseases
- Protein Hydrolysis and Bioactive Peptides
- Identification and Quantification in Food
- Polysaccharides and Plant Cell Walls
- Plant Pathogenic Bacteria Studies
- Enzyme Production and Characterization
- Microbial Community Ecology and Physiology
- Microbial Inactivation Methods
- Food Safety and Hygiene
- Amino Acid Enzymes and Metabolism
- Mycorrhizal Fungi and Plant Interactions
- Tea Polyphenols and Effects
- Sirtuins and Resveratrol in Medicine
- Pediatric health and respiratory diseases
- Bacillus and Francisella bacterial research
- Fermentation and Sensory Analysis
- Enterobacteriaceae and Cronobacter Research
Max Rubner Institut
2014-2024
Karlsruhe Institute of Technology
2013
This study aimed to evaluate the safety of raw vegetable products present on German market regarding toxin-producing Bacillus cereus sensu lato (s.l.) group bacteria.A total 147 B. s.l. strains isolated from cucumbers, carrots, herbs, salad leaves and ready-to-eat mixed were analyzed. Their toxinogenic potential was assessed by multiplex PCR targeting hemolysin BL (hbl) component D (hblD), non-hemolytic enterotoxin (nhe) A (nheA), cytotoxin K-2 (cytK-2) cereulide (ces) toxin genes. In...
Maillard reaction products (MRPs) are taken up in substantial amounts with the daily diet, but majority not transported across intestinal epithelium. The aim of this study was to obtain first insights into stability dietary MRPs presence microbiota. Four individual MRPs, namely, N-ε-fructosyllysine (FL), N-ε-carboxymethyllysine (CML), pyrraline (PYR), and maltosine (MAL), were anaerobically incubated fecal suspensions from eight human volunteers at 37 °C for 72 h. measured by HPLC UV MS/MS...
Pectins are dietary fibers that attributed with several beneficial immunomodulatory effects. Depending on the degree of esterification (DE), pectins can be classified as high methoxyl pectin (HMP) or low (LMP). The aim this study was to investigate effects methyl-esterification intestinal microbiota and its properties in naive mice. Supplementation diet LMP HMP induced changes composition mice toward Bacteroides, which mainly promoted by HMP. Metabolome analysis stool samples from pectin-fed...
The intake of isoflavones is presumed to be associated with health benefits in humans, but also potential adverse effects are controversially discussed. Isoflavones can metabolized by gut bacteria leading modulation the bioactivity, such as estrogenic effects. Especially bacterial strains Eggerthellaceae, a well-known family human microbiota, able convert isoflavone daidzein into equol. In addition, metabolization genistein described for Eggerthellaceae. aim this study was identify and...
The study aimed to evaluate the effect of bacteriocins produced by Lactobacillus sakei CWBI-B1365 and curvatus CWBI-B28 on growth survival Listeria monocytogenes in raw beef poultry meat.The sakacin P G structural genes were identified Lact. using PCR amplification, respectively. two bacteriocinogenic strains either alone or together, that nonbacteriocin-producing strain LMG17302, L. was evaluated meat. In beef, pathogenic bacteria inhibited strains. had no activity chicken meat when...
Eggerthella and Slackia spp. are gut associated bacteria that have been suggested to play roles in host lipid xenobiotic metabolism. A quantitative PCR method for the selective enumeration of belonging either genus or was developed order establish numbers these occurring human feces. The primers amplification genera were tested first conventional test their specificity. Representative species Slackia, as well closely related Coriobacteriia, included investigation. selected shown be capable...
Abstract A total of 57 predominant LAB strains isolated from cassava and maize grains fermentation processes for fufu ogi were identified using phenotypic genomic fingerprinting methods such as rep-PCR ARDRA. They divided into facultatively heterofermentative rods (26.3%), obligately (31.6%), tetrad forming homofermentative cocci (42.1%). Selected further by sequencing the 16S rDNA gene. Technological studies acidification, hydrogen peroxide production, starch hydrolysis, enzymatic...
Two novel, anaerobic, Gram-positive, rod-shaped bacterial strains, ResAG-85T and ResAG-96T, were isolated from a faecal sample of male human. 16S rRNA gene sequences analyses indicated that these strains represent distinct lineage within the family Eggerthellaceae. Strain showed 92.3 % similarity to type genera Eggerthella Gordonibacter. ResAG-96T clustered together with Paraeggerthella hongkongensis newly (but not validly) published genus 'Arabia massiliensis' (94.8 similarity). Analysis...
A genus-specific PCR analysis method was developed for a rapid and reliable differentiation between the two heterofermentative lactic acid bacteria genera Leuconostoc Weissella. Primer sets specific target regions of 16S rRNA genes were designed specificity evaluated using type strains 13 species 11 In addition, newly applied to characterize 72 (LAB) isolated from coffee fermentation which presumptively classified as or Weissella species. Additionally, total 34 LAB isolates various other...
The novel, Gram-stain-positive, ovoid, lactic acid bacterial isolates LMG 27205, 27206, 27207(T) and MRI-F 18 were obtained from throat samples of healthy humans. 16S rRNA gene sequence analyses indicated that these belong to the genus Streptococcus, specifically Streptococcus mitis group, with australis as nearest neighbours (99.45 98.56 % similarity respective type strains). Genotypic fingerprinting by fluorescent amplified fragment length polymorphism (FAFLP) pulsed-field gel...
To investigate effects of salinity stress on growth, nutrient status, chlorophyll and water consumption, four different saline irrigation waters (S0= 0.3 dS/m-1 control, S1=1.5 dS/m-1, S2=3 S3=5 dS/m-1) were applied to two-year Chandler saplings which grafted Juglans nigra L. (JN), Paradox (PR) sieoboldiana (JS) walnut rootstocks. Three salts including NaCl, MgSO4, CaCl2 used prepare irrigations. Shoot length change rate (%) was varied between 77.57 (JN) 81.83 for S0 treatments 55.60 56.84...
A total of 189 samples fresh products (leafy salads, ready-to-eat mixed and herbs) bought in retail Southwest Germany were investigated for their microbiological quality the presence pathogenic bacteria, including Salmonella spp., Listeria monocytogenes, presumptive Bacillus cereus. Total aerobic mesophilic plate counts (TAC) ranged from 5.5 to 9.6 log colony-forming units (CFUs) per gram. Enterobacteria pseudomonads predominant microorganisms detected all with between 5.0 9.2 CFU/g. Strains...