A5025499049- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Plant Pathogenic Bacteria Studies
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Microbial Metabolism and Applications
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Gut microbiota and health
- Enzyme Production and Characterization
- Enterobacteriaceae and Cronobacter Research
- Listeria monocytogenes in Food Safety
- Aquaculture disease management and microbiota
- Plant-Microbe Interactions and Immunity
- Meat and Animal Product Quality
- Biosensors and Analytical Detection
- Bacillus and Francisella bacterial research
- Food composition and properties
- Collagen: Extraction and Characterization
- Biopolymer Synthesis and Applications
- Antimicrobial Peptides and Activities
- Microbial Community Ecology and Physiology
- Escherichia coli research studies
- Microbial Metabolites in Food Biotechnology
Max Rubner Institut
2016-2025
Max Planck Institute for Demographic Research
2014
Summary Schedules of survival, growth and reproduction are key life‐history traits. Data on how these traits vary among species populations fundamental to our understanding the ecological conditions that have shaped plant evolution. Because demographic schedules determine population or decline, such data help us understand different biomes shape ecology, communities respond global change develop successful management tools for endangered invasive species. Matrix models summarize life cycle...
This study aimed to evaluate the safety of raw vegetable products present on German market regarding toxin-producing Bacillus cereus sensu lato (s.l.) group bacteria.A total 147 B. s.l. strains isolated from cucumbers, carrots, herbs, salad leaves and ready-to-eat mixed were analyzed. Their toxinogenic potential was assessed by multiplex PCR targeting hemolysin BL (hbl) component D (hblD), non-hemolytic enterotoxin (nhe) A (nheA), cytotoxin K-2 (cytK-2) cereulide (ces) toxin genes. In...
Tuna cans are relevant seafood products for which mixtures of different tuna species not allowed according to European regulations. In order support the prevention food fraud and mislabelling, a next-generation sequencing methodology based on mitochondrial cytochrome b control region markers has been tested. Analyses defined DNA, fresh tissue canned revealed qualitative and, some extent, semiquantitative identification species. While choice bioinformatic pipeline had no influence in results...
This study aimed to characterize antibiotic-resistance plasmids present in microorganisms from sprout samples using exogenous plasmid capture. Fresh mung bean sprouts were predominantly colonized by bacteria the phyla Proteobacteria and Bacteroidetes . To capture plasmids, a plasmid-free Escherichia ( E. ) coli CV601 strain, containing green fluorescent protein gene for selection, was used as recipient strain experiments. Transconjugants selected on media cefotaxime or tetracycline...
This study re-evaluates the microbial degradation of rutin and genistein, emphasizing health-related metabotypes novel transformation products these compounds. In silico predictions calculations were made to determine molecular formulae metabolites derived from precursor molecules by methylation, sulfation, dehydroxylation. To validate these, anaerobic, ex vivo experiments conducted with pooled fecal samples obese non-obese test persons (n=7 per group) over 48 hours, followed 16S rRNA gene...
<title>Abstract</title> Objectives: <italic>Salmonella enterica</italic> is a globally significant foodborne pathogen and leading cause of gastrointestinal infections, with increasing concern over strains harboring antimicrobial resistance (AMR). This Data Note reports draft genome sequences six <italic>S. subsp. <italic>enterica</italic> isolates from fresh produce agricultural environments in South Korea. The objective this work was to provide genomic data on environmental...
Nono, an important traditional fermented dairy food produced from cow’s milk in Nigeria, was studied for microbial diversity and starter culture development industrial production. On the basis of a polyphasic approach, including phenotypic genotypic methods such as 16S rRNA gene sequencing, repetitive element PCR (rep-PCR) fingerprinting metagenomics, whole genome we identified Lactobacillus (Lb.) helveticus, Limosilactobacillus (L.) fermentum, Lb. delbrueckii, Streptococcus (S.)...
Abstract Most bacterial identification methods require extensive culturing, strain purification and DNA extraction protocols. This leads to additional expenses time lags when isolating specific bacteria from complex microbiological ecosystems. study aimed develop a fast robust method for of lactobacilli, bifidobacteria Bacteroides in human faecal samples. Bacteria samples were cultured anaerobically on selective media. Sonication-based was performed, followed by almost complete 16S rRNA gene...
We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities companies) from Germany Austria. Participants were asked to sequence six DNA samples three bacterial species (Campylobacter jejuni, Listeria monocytogenes Salmonella enterica) duplicate, according their routine in-house protocol. Four different types Illumina platforms (MiSeq, NextSeq, iSeq,...
Abstract The German National Reference Centre for Authentic Food (NRZ-Authent) and the competent food control authorities of federal states cooperated within framework 10th joint Europol INTERPOL operation OPSON (OPSON X) in detection adulterated meat products. A total 63 product samples were collected analysed by using standard analytical procedures subjected to a recently published 16S rDNA metabarcoding analysis. sequence reads 3 bioinformatics data processing strategies. study aimed gain...
PMBT24, the first reported virulent bacteriophage infecting anaerobic human gut bacterium
Here we present the description of a novel Pseudomonas species, designated rustica sp. nov., which was isolated from raw milk samples obtained Germany. Results initial 16S rRNA gene sequence analysis assigned strain into genus and showed helmanticensis, neuropathica atagonensis to be its closest relatives. Further studies including rpoB gene, multi-gene phylogenetic tree reconstruction, whole-genome comparisons, cellular fatty acid chemotaxonomic characterization clear separation known...
In this work, we report the draft whole-genome sequence of multiply antibiotic-resistant Citrobacter portucalensis strain MBTC-1222 isolated from uziza leafy vegetable in Nigeria. Sequence analysis showed assembled genome size to be 4,881,935 bp, containing 4,603 protein-coding genes, 131 pseudogenes, 7 rRNAs, 74 tRNAs, and 9 noncoding RNAs (ncRNAs).
Abstract Sodium intake is a major risk factor for non-communicable diseases. Consequently, reformulation of cheeses such as Edam to contain less sodium may contribute lowering disease risk. However, essential cheese manufacture, influencing starter culture bacteria activity and abundance during fermentation. This study aimed assess the microbial diversity reformulated samples with reduced content using culture-independent technique. The produced simple reduction, well by substituting salt...
For successful elucidation of a food-borne infection chain, the availability high-quality sequencing data from suspected microbial contaminants is prerequisite. Commonly, those investigations are joint effort undertaken by different laboratories and institutes. To analyze extent variability introduced differing wet-lab procedures on quality sequence we conducted an interlaboratory study, involving four bacterial pathogens, which account for majority food-related infections: Campylobacter...
ABSTRACT In this study, the genomes of 22 Enterobacteriaceae isolates from fresh produce and herbs obtained retail markets in northern Germany were completely sequenced with MiSeq short-read MinION long-read sequencing assembled using a Unicycler hybrid assembly. The data showed that 17 strains harbored between one five plasmids, whereas strains, only circular chromosomal DNA was detected. total, 38 plasmids identified. size detected varied ca. 2,000 326,000 bp, heavy metal resistance genes...
A bacteriophage virulent for extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae strain 182 was isolated from sewage. The double-stranded DNA (dsDNA) genome showed high similarity to the genomes of other phages. It comprises 175,206 bp with a mol% G+C content 41.9 and contains 276 putative open reading frames (ORFs) one tRNA.