A5073621219- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Long-Term Effects of COVID-19
- Infectious Encephalopathies and Encephalitis
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Viral Infections and Immunology Research
- Olfactory and Sensory Function Studies
- Respiratory viral infections research
- Peroxisome Proliferator-Activated Receptors
- Neuroinflammation and Neurodegeneration Mechanisms
- Biosensors and Analytical Detection
- Peripheral Neuropathies and Disorders
- RNA regulation and disease
- Influenza Virus Research Studies
- Plant Virus Research Studies
- Virology and Viral Diseases
- COVID-19 diagnosis using AI
- Poxvirus research and outbreaks
- Endoplasmic Reticulum Stress and Disease
- Cholesterol and Lipid Metabolism
- Nerve injury and regeneration
- Polyomavirus and related diseases
Centre Hospitalier Universitaire Sainte-Justine
2020-2022
Centre Intégré Universitaire de Santé et de Services Sociaux du Saguenay–Lac-Saint-Jean
2022
Université de Montréal
1997-2022
Université du Québec à Montréal
1991-2022
Centre Intégré Universitaire de Santé et de Services Sociaux du Centre-Sud-de-l'Île-de-Montréal
2022
Jacksonville College
2022
Mayo Clinic in Florida
2022
WinnMed
2022
McGill Genome Centre
2022
Institut National de la Recherche Scientifique
2011-2021
Human coronaviruses (HCoVs) are recognized respiratory pathogens for which accumulating evidence indicates that in vulnerable patients the infection can cause more severe pathologies. HCoVs not always confined to upper tract and invade central nervous system (CNS) under still unclear circumstances. HCoV-induced neuropathologies humans difficult diagnose early enough allow therapeutic interventions. Making use of our already described animal model HCoV neuropathogenesis, we describe route...
HighlightsAntibodies against SARS-CoV-2 Spike correlate with COVID-19 severityRBD-specific IgM and IgA decline more rapidly than IgGSARS-CoV-2 neutralizing antibodies are elicited within 2 weeks of infectionNeutralizing significantly after resolution the infectionSummarySARS-CoV-2 is responsible for coronavirus disease 2019 (COVID-19) pandemic, infecting millions people causing hundreds thousands deaths. The glycoproteins mediate viral entry main targets antibodies. Understanding antibody...
The coronavirus S-protein mediates receptor binding and fusion of the viral host cell membranes. In HCoV-229E, its domain (RBD) shows extensive sequence variation but how function is maintained not understood. Reported are X-ray crystal structures Class III-V RBDs in complex with human aminopeptidase N (hAPN), as well electron cryomicroscopy structure 229E S-protein. show that common core interactions define specificity for hAPN peripheral RBD accommodated by loop plasticity. results provide...
RNA viruses are characterized by a high mutation rate, buffer against environmental change. Nevertheless, the means which random improves viral fitness is not well characterized. Here we report X-ray crystal structure of receptor-binding domain (RBD) human coronavirus, HCoV-229E, in complex with ectodomain its receptor, aminopeptidase N (APN). Three extended loops solely responsible for receptor binding and evolution HCoV-229E close relatives accompanied changing loop-receptor interactions....
The coronavirus SARS-CoV-2 (SCV2) causes acute respiratory distress, termed COVID-19 disease, with substantial morbidity and mortality. As SCV2 is related to previously-studied coronaviruses that have been shown the capability for brain invasion, it seems likely may be able do so as well. To date, although there many clinical autopsy-based reports describe a broad range of SCV2-associated neurological conditions, unclear what fraction these due direct CNS invasion versus indirect effects...
The complete genome sequences of the human coronavirus OC43 (HCoV-OC43) laboratory strain from American Type Culture Collection (ATCC), and a HCoV-OC43 clinical isolate, designated Paris, were obtained. Both genomes are 30,713 nucleotides long, excluding poly(A) tail, only differ by 6 nucleotides. These six mutations scattered throughout give rise to two amino acid substitutions: one in spike protein gene (I958F) other nucleocapsid (V81A). Furthermore, variants shown reach central nervous...
Coronaviruses are a family of enveloped single-stranded positive-sense RNA viruses causing respiratory, enteric, and neurologic diseases in mammals fowl. Human coronaviruses recognized to cause up third common colds suspected be involved enteric diseases. Coronavirus replication involves the generation nested subgenomic mRNAs (sgmRNAs) with capped 5' leader sequence. The translation most sgmRNAs is thought cap dependent displays requirement for eukaryotic initiation factor 4F (eIF4F),...
Human coronaviruses (HCoV) are respiratory pathogens that may be associated with the development of neurological diseases, in view their neuroinvasive and neurotropic properties. The viral spike (S) glycoprotein is a major virulence factor for several coronavirus species, including OC43 strain HCoV (HCoV-OC43). In an attempt to study role this protein virus spread within central nervous system (CNS) neurovirulence, as well identify amino acid residues important such functions, we compared...
Brains of 42 COVID-19 decedents and 107 non-COVID-19 controls were studied. RT-PCR screening 16 regions from 20 autopsies found SARS-CoV-2 E gene viral sequences in 7 (2.5% 320 samples), concentrated 4/20 subjects (20%). Additional olfactory bulb (OB), amygdala (AMY) entorhinal area for E, N1, N2, RNA-dependent RNA polymerase, S detected one or more these OB 8/21 (38%). It is uncertain whether represent viable virus. Significant histopathology was limited to 2/42 cases (4.8%), with a large...
This study describes the assembly of a full-length cDNA clone human coronavirus (HCoV)-OC43 in bacterial artificial chromosome (BAC). The BAC containing infectious (pBAC-OC43(FL)) was assembled using two-part strategy. first step consisted introduction each end viral genome into with accessory sequences allowing proper transcription. second insertion whole HCoV-OC43 BAC. To produce recombinant particles, pBAC-OC43(FL) transfected BHK-21 cells. Recombinant virus displayed same phenotypic...
Human coronaviruses (HCoV) are recognized respiratory pathogens. Some HCoV strains, including HCoV-OC43, can invade the central nervous system, where they infect neurons, with unclear consequences. We have previously reported that HCoV-OC43 infection of human neurons activates unfolded-protein response and caspase-3 induces cell death viral spike (S) glycoprotein is involved in process. now report on underlying mechanisms associated induction programmed (PCD) after by reference virus...
ABSTRACT Human coronaviruses (HCoV) are recognized respiratory pathogens, and some strains, including HCoV-OC43, can infect human neuronal glial cells of the central nervous system (CNS) activate neuroinflammatory mechanisms. Moreover, HCoV-OC43 is neuroinvasive, neurotropic, neurovirulent in susceptible mice, where it induces chronic encephalitis. Herein, we show that a single point mutation viral spike (S) glycoprotein (Y241H), acquired during persistence neural cells, led to hind-limb...
Most betacoronaviruses possess an hemagglutinin-esterase (HE) protein, which appears to play a role in binding or release from the target cell. Since this HE protein possesses acetyl-esterase activity that removes acetyl groups O-acetylated sialic acid, as receptor-destroying enzyme has been postulated. However, precise function of and its enzymatic remains poorly understood. Making use neutralizing antibody molecular clones recombinant human coronavirus OC43 (HCoV-OC43), our results suggest...
Human coronaviruses (HCoVs) are recognized respiratory pathogens with neuroinvasive and neurotropic properties in mice humans. HCoV strain OC43 (HCoV-OC43) can infect persist human neural cells activate neuroinflammatory neurodegenerative mechanisms, suggesting that it could be involved neurological disease of unknown etiology Moreover, we have shown HCoV-OC43 is neurovirulent susceptible mice, causing encephalitis, a viral mutant single point mutation the surface spike (S) protein induces...
Human coronaviruses (HCoV) are respiratory pathogens with neuroinvasive, neurotropic, and neurovirulent properties, highlighting the importance of studying potential implication these viruses in neurological diseases. The OC43 strain (HCoV-OC43) was reported to induce neuronal cell death, which may participate neuropathogenesis. Here, we show that HCoV-OC43 harboring two point mutations spike glycoprotein (rOC/Us183-241) more than wild-type (rOC/ATCC) mice induced death murine human cells....
ABSTRACT Human coronaviruses (HCoVs) cause 15 to 30% of mild upper respiratory tract infections. However, no specific antiviral drugs are available prevent or treat HCoV infections date. Here, we developed four infectious recombinant HCoVs-OC43 (rHCoVs-OC43) which express the Renilla luciferase (Rluc) reporter gene. Among these rHCoVs-OC43, rOC43-ns2DelRluc (generated by replacing ns2 with Rluc gene) showed robust activity only a slight impact on its growth characteristics. Additionally,...
The SARS-CoV-2 virus is responsible for the current worldwide coronavirus disease 2019 (COVID-19) pandemic, infecting millions of people and causing hundreds thousands deaths. Spike glycoprotein mediates viral entry main target neutralizing antibodies. Understanding antibody response directed against crucial development vaccine, therapeutic public health interventions. Here we performed a cross-sectional study on 106 SARS-CoV-2-infected individuals to evaluate humoral responses Spike. vast...
The SBM mouse is a unique transgenic model of polycystic kidney disease (PKD) induced by the dysregulated expression c-myc in renal tissue. In situ hybridization analysis demonstrated intense signal for transgene overlying tubular cystic epithelium mice. Renal proliferation index kidneys was 10-fold increased over nontransgenic controls correlating with presence epithelial hyperplasia. specificity proliferative potential cells substitution proto-oncogene c-fos or transforming growth factor...