A5004646557- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Muscle Physiology and Disorders
- SARS-CoV-2 detection and testing
- Antibiotic Resistance in Bacteria
- Viral Infections and Vectors
- Antibiotics Pharmacokinetics and Efficacy
- RNA Research and Splicing
- Protease and Inhibitor Mechanisms
- COVID-19 Clinical Research Studies
- Signaling Pathways in Disease
- Virology and Viral Diseases
- Cardiomyopathy and Myosin Studies
- Antimicrobial Resistance in Staphylococcus
- Nuclear Structure and Function
- Malaria Research and Control
- Peptidase Inhibition and Analysis
- Cultural Insights and Digital Impacts
- Poxvirus research and outbreaks
- Virus-based gene therapy research
- Phenothiazines and Benzothiazines Synthesis and Activities
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Diverse Cultural and Historical Studies
- Synthesis of β-Lactam Compounds
Institut National de Santé Publique
2021-2024
Ste. Anne's Hospital
2023-2024
Institut National de Santé Publique du Québec
2016-2023
Montreal Neurological Institute and Hospital
2006-2012
McGill University
2006-2011
New England Biolabs (United States)
2008
Duke University
2008
National Academies of Sciences, Engineering, and Medicine
2001
University of Michigan
2001
Biotechnology Research Institute
2001
HighlightsAntibodies against SARS-CoV-2 Spike correlate with COVID-19 severityRBD-specific IgM and IgA decline more rapidly than IgGSARS-CoV-2 neutralizing antibodies are elicited within 2 weeks of infectionNeutralizing significantly after resolution the infectionSummarySARS-CoV-2 is responsible for coronavirus disease 2019 (COVID-19) pandemic, infecting millions people causing hundreds thousands deaths. The glycoproteins mediate viral entry main targets antibodies. Understanding antibody...
Dysferlin is a type II transmembrane protein implicated in Ca2+-dependent sarcolemmal membrane repair. has seven C2 domains, which are lipid and binding modules. In this study, we sought to characterize the specificity of dysferlin's domains. Dysferlin's C2A domain was able bind phosphatidylserine (PS), phosphatidylinositol 4-phosphate [PtdIns(4)P], 4,5-bisphosphate [PtdIns(4,5)P2] fashion. The remainder domains exhibited weaker Ca2+-independent PS no significant phosphoinositides.
The SARS-CoV-2 virus is responsible for the current worldwide coronavirus disease 2019 (COVID-19) pandemic, infecting millions of people and causing hundreds thousands deaths. Spike glycoprotein mediates viral entry main target neutralizing antibodies. Understanding antibody response directed against crucial development vaccine, therapeutic public health interventions. Here we performed a cross-sectional study on 106 SARS-CoV-2-infected individuals to evaluate humoral responses Spike. vast...
Restriction endonucleases are the basic tools of molecular biology. Many restriction show relaxed sequence recognition, called star activity, as an inherent property under various digestion conditions including optimal ones. To quantify this we propose concept Fidelity Index (FI), which is defined ratio maximum enzyme amount showing no activity to minimum needed for complete at cognate recognition site any particular endonuclease. indices a large number reported here. The effects reaction...
Dysferlin is a type II transmembrane protein implicated in surface membrane repair muscle. Mutations dysferlin lead to limb girdle muscular dystrophy 2B, Miyoshi Myopathy and distal anterior compartment myopathy. Dysferlin's mode of action not well understood only few binding partners have thus far been identified. Using affinity purification followed by liquid chromatography/mass spectrometry, we identified alpha-tubulin as novel partner for dysferlin. The association between alpha-tubulin,...
As the coronavirus disease 2019 (COVID-19) pandemic second wave is emerging, it of upmost importance to screen population immunity in order keep track infected individuals. Consequently, immunoassays for severe acute respiratory syndrome 2 (SARS-CoV-2) with high specificity and positive predictive values are needed obtain an accurate epidemiological picture. more data accumulate about immune responses kinetics neutralizing-antibody (nAb) production SARS-CoV-2-infected individuals, new...
The authors report a genotype-phenotype correlation in limb-girdle muscular dystrophy 2B family. Two severely affected sisters were homozygous for dysferlin null mutation. Their mildly compound heterozygous mother harbored, addition to one allele, an in-frame exon-skipping allele caused by novel lariat branch point molecule arising from the latter appeared partially complement mutation, likely accounting mother9s mild phenotype.
Dysferlin is a multi-C2 domain transmembrane protein involved in plethora of cellular functions, most notably skeletal muscle membrane repair, but also myogenesis, adhesion and intercellular calcium signaling. We previously showed that dysferlin interacts with alpha-tubulin microtubules cells. Microtubules are heavily reorganized during myogenesis to sustain growth elongation the nascent fiber. Microtubule function regulated by post-translational modifications, such as acetylation its...
Climate change is likely to increase the risk of human transmission arboviruses endemic Canada, including West Nile virus (WNV), Eastern equine encephalitis (EEEV) and California serogroup (CSV), calling for enhanced surveillance, entomological surveillance targeting mosquito vectors. A scoping review was carried out document public health contributions importance in Canada.
Several new cysteine proteases of the papain family have been discovered in past few years. To help assignment physiological roles and design specific inhibitors, a clear picture specificities these enzymes is needed. One novel enzymes, cathepsin X, displays unique specificity, cleaving single amino acid residues at C-terminus substrates very efficiently. In this study, carboxypeptidase activities substrate specificity cathepsins X B investigated detail compared. Using quenched fluorogenic...
The COVID-19 pandemic has led to the influx of immunoassays for detection antibodies towards severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into global market. Canadian Public Health Laboratory Network Serology Task Force undertook a nationwide evaluation twelve laboratory and 6 point-of-care based commercial serological assays SARS-CoV-2 antibodies. We determined that there was considerable variability in performance individual tests an orthogonal testing algorithm should be...
Jamestown Canyon virus (JCV) is a mosquitoborne orthobunyavirus in the California serogroup that circulates throughout Canada and United States. Most JCV exposures result asymptomatic infection or mild febrile illness, but can also cause neurologic diseases, such as meningitis encephalitis. We describe case series of confirmed JCV-mediated neuroinvasive disease among persons from provinces British Columbia, Alberta, Quebec, Nova Scotia, Canada, during 2011-2016. highlight definitions,...
Abstract Danon disease is a rare X‐linked dominant disorder caused by lysosomal‐associated membrane protein 2 (LAMP2) deficiency and characterized hypertrophic cardiomyopathy, cardiac conduction abnormalities, skeletal vacuolar myopathy, variable degree of mental retardation, peripheral pigmentary retinopathy. We describe novel splice mutation in the LAMP2 gene French Canadian family. By identifying this we were able to offer genetic screening counseling all family members. Presymptomatic...
Serological assays designed to detect SARS-CoV-2 antibodies are being used in serological surveys and other specialized applications. As a result, ensure that the outcomes of testing meet high quality standards, evaluations required assess performance these proficiency laboratories performing them.A panel 60 plasma/serum samples from blood donors who had reverse transcriptase-polymerase chain reaction (RT-PCR) confirmed infections 21 negative were secured distributed interested within Canada...
Background: There is a paucity of data on the dynamics human papillomavirus (HPV) antibodies in children. We aimed to describe vertical transmission and clearance against HPV6, 11, 16 18 children.Methods: used from pregnant women recruited into HERITAGE cohort study between 2009 2012 who were positive for HPV-DNA at baseline. Dried blood spots collected during first trimester participants, birth, 6, 12, 24 months age The level total immunoglobulin G (IgG) measured using Luminex immunoassays....
The PSE-4 enzyme is a prototype carbenicillin-hydrolyzing exhibiting high activity against penicillins and early cephalosporins. To understand the mechanism that modulates substrate profiles to verify ability of extend its specificity toward expanded-spectrum cephalosporins, we used random replacement mutagenesis generate six libraries from amino acids 162 179 in Omega loop. This region known studies with TEM-1 be implicated specificity. It was found modulating ceftazidime hydrolysis...
The carboxypeptidase and endopeptidase activities of cathepsins X B, as well their inhibition by E-64 derivatives, have been investigated in detail compared. results clearly demonstrate that B do not share similar activity profiles against substrates inhibitors. Using quenched fluorogenic substrates, we show cathepsin preferentially cleaves through a monopeptidyl pathway, while displays preference for the dipeptidyl pathway. one or other pathway is about same both enzymes, i. e....