Guillaume Beaudoin-Bussières
A5017617949- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- HIV Research and Treatment
- Long-Term Effects of COVID-19
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- COVID-19 Impact on Reproduction
- Kawasaki Disease and Coronary Complications
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Conservation, Biodiversity, and Resource Management
- COVID-19 epidemiological studies
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Bacillus and Francisella bacterial research
- Vaccine Coverage and Hesitancy
- Viral Infections and Outbreaks Research
- Oil Palm Production and Sustainability
- Mechanical Circulatory Support Devices
- HIV/AIDS Research and Interventions
- French Urban and Social Studies
Centre Hospitalier de l’Université de Montréal
2020-2025
Université de Montréal
2020-2025
Bipar
2021
University of Florida
2021
Center for International Forestry Research
2014-2016
Abstract The efficacy of convalescent plasma for coronavirus disease 2019 (COVID-19) is unclear. Although most randomized controlled trials have shown negative results, uncontrolled studies suggested that the antibody content could influence patient outcomes. We conducted an open-label, trial adults with COVID-19 receiving oxygen within 12 d respiratory symptom onset ( NCT04348656 ). Patients were allocated 2:1 to 500 ml or standard care. composite primary outcome was intubation death by 30...
HighlightsAntibodies against SARS-CoV-2 Spike correlate with COVID-19 severityRBD-specific IgM and IgA decline more rapidly than IgGSARS-CoV-2 neutralizing antibodies are elicited within 2 weeks of infectionNeutralizing significantly after resolution the infectionSummarySARS-CoV-2 is responsible for coronavirus disease 2019 (COVID-19) pandemic, infecting millions people causing hundreds thousands deaths. The glycoproteins mediate viral entry main targets antibodies. Understanding antibody...
In the absence of effective vaccines and with limited therapeutic options, convalescent plasma is being collected across globe for potential transfusion to coronavirus disease 2019 (COVID-19) patients. The therapy has been deemed safe, several clinical trials assessing its efficacy are ongoing. While it remains be formally proven, presence neutralizing antibodies thought play a positive role in this treatment. Indeed, titers ≥1:160 have recommended some inclusion. Here, we performed repeated...
With the recent approval of highly effective coronavirus disease 2019 (COVID-19) vaccines, functional and lasting immunity to severe acute respiratory syndrome 2 (SARS-CoV-2) is currently under investigation as antibody levels in plasma were shown decline during convalescence. Since absence antibodies does not equate immune memory, we evaluate presence SARS-CoV-2-specific memory B cells convalescent individuals. Here, report a longitudinal assessment humoral responses on 32 donors up 8...
Characterization of the humoral response to SARS-CoV-2, etiological agent COVID-19, is essential help control infection. The neutralization activity plasma from patients with COVID-19 decreases rapidly during first weeks after recovery. However, specific role each immunoglobulin isotype in overall neutralizing capacity still not well understood. In this study, we select a cohort convalescent and selectively deplete A, M, or G before testing remaining depleted plasma. We find that depletion M...
Emerging evidence indicates that both neutralizing and Fc-mediated effector functions of antibodies contribute to protection against SARS-CoV-2. It is unclear whether Fc-effector alone can protect Here, we isolated CV3-13, a non-neutralizing antibody, from convalescent individual with potent functions. The cryoelectron microscopy structure CV3-13 in complex the SARS-CoV-2 spike reveals antibody binds distinct angle approach an N-terminal domain (NTD) epitope only partially overlaps NTD...
Continuous emergence of SARS-CoV-2 variants concern (VOCs) is fueling the COVID-19 pandemic. Omicron (B.1.1.529) rapidly spread worldwide. The large number mutations in its Spike raise concerns about a major antigenic drift that could significantly decrease vaccine efficacy and infection-induced immunity. A long interval between BNT162b2 mRNA doses elicits antibodies efficiently recognize Spikes from different VOCs. Here, we evaluate recognition by plasma cohort naive previously infected...
Throughout the SARS-CoV-2 pandemic, several variants of concern (VOCs) have been identified, many which share recurrent mutations in spike glycoprotein’s receptor-binding domain (RBD). This region coincides with known epitopes and can therefore an impact on immune escape. Protracted infections immunosuppressed patients hypothesized to lead enrichment such drive evolution towards VOCs. Here, we present case patient that developed distinct populations escape throughout course their infection....
Statistical models of plasma immunovirological features validate SARS-CoV-2 vRNA as an early predictor COVID-19 mortality.
Abstract The standard dosing of the Pfizer/BioNTech BNT162b2 mRNA vaccine validated in clinical trials includes two doses administered three weeks apart. While decision by some public health authorities to space because limiting supply has raised concerns about efficacy, data indicate that a single dose is up 90% effective starting 14 days after its administration. We analyzed humoral and T cells responses this vaccine. Despite proven efficacy at time point, no neutralizing activity were...
Small CD4-mimetic compounds (CD4mc) sensitize HIV-1-infected cells to antibody-dependent cellular cytotoxicity (ADCC) by facilitating antibody recognition of epitopes that are otherwise occluded on the unliganded viral envelope (Env). Combining CD4mc with two families CD4-induced (CD4i) antibodies, which frequently found in plasma individuals, stabilizes Env a conformation is vulnerable ADCC. We employed new-generation SRG-15 humanized mice, supporting natural killer (NK) cell and...
Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) surrogate neutralization assays that obviate the need for viral culture offer substantial advantages regarding throughput and cost. The cPass SARS-CoV-2 Neutralization Antibody Detection Kit (GenScript) is first such commercially available assay detects antibodies block receptor-binding domain (RBD)/angiotensin-converting enzyme (ACE)-2 interaction. We aimed to evaluate inform its use assess added value compared with...
Soluble angiotensin-converting enzyme 2 (ACE2) constitutes an attractive antiviral capable of targeting a wide range coronaviruses using ACE2 as their receptor. Using structure-guided approaches, we developed series bivalent ACE2-Fcs harboring functionally and structurally validated mutations that enhance severe acute respiratory syndrome coronavirus (SARS-CoV-2) receptor binding domain recognition by up to ~12-fold remove angiotensin enzymatic activity. The lead variant M81 potently...
COVID-19 convalescent plasmas (CCPs) are chosen for plasma therapy based on neutralizing titers and anti-Spike immunoglobulin levels. However, CCP characteristics that promote SARS-CoV-2 control complex incompletely defined. Using an in vivo imaging approach, we demonstrate CCPs with low (ID50 ≤ 1:250), but moderate to high Fc-effector activity, contrast those poor Fc function, delay mortality and/or improve survival of SARS-CoV-2-challenged K18-hACE2 mice. The impact innate immune cells...
Although official on-the-ground environmental monitoring is absent over much of the world, many people living in these regions observe, manage, and protect their environment.The autonomous processes associated with activities are seldom documented appear poorly recognized by conservation professionals.We identified three villages Mamberamo-Foja region (Mamberamo Regency) Papua (Indonesian New Guinea).In each village we found evidence that local contributes to effective protection deters...
The SARS-CoV-2 virus is responsible for the current worldwide coronavirus disease 2019 (COVID-19) pandemic, infecting millions of people and causing hundreds thousands deaths. Spike glycoprotein mediates viral entry main target neutralizing antibodies. Understanding antibody response directed against crucial development vaccine, therapeutic public health interventions. Here we performed a cross-sectional study on 106 SARS-CoV-2-infected individuals to evaluate humoral responses Spike. vast...
Antibodies can engage specific receptors at the surface of effector cells and mediate several functions beyond viral neutralization. Increasing evidence suggests that Fc-mediated functions, such as antibody-dependent cellular cytotoxicity (ADCC), have an important role in protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. We engineered a cell line stably expressing GFP-tagged SARS-CoV-2 spike to measure ADCC. This protocol provides optimized way...