A5100447526- Pluripotent Stem Cells Research
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Reproductive Biology and Fertility
- Cancer Cells and Metastasis
- Genetics and Neurodevelopmental Disorders
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Wnt/β-catenin signaling in development and cancer
- Autism Spectrum Disorder Research
- Cancer Risks and Factors
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Shoulder Injury and Treatment
- Anesthesia and Pain Management
- Gut microbiota and health
- Spine and Intervertebral Disc Pathology
- Animal Genetics and Reproduction
Center for Excellence in Molecular Cell Science
2015-2024
University of Chinese Academy of Sciences
2018-2024
Xi'an Jiaotong University
2024
Chinese Academy of Sciences
2015-2023
Southern University of Science and Technology
2023
Jinan University
2023
Chinese University of Hong Kong
2023
Shanghai Institutes for Biological Sciences
2015-2016
Abstract The pluripotency of mammalian early and late epiblast could be recapitulated by naïve embryonic stem cells (ESCs) primed (EpiSCs), respectively. However, these two states may not sufficient to reflect the full complexity developmental potency during development. Here we report establishment self-renewing formative pluripotent (fPSCs) which manifest features poised for gastrulation. fPSCs can established from different mouse ESCs, pre-/early-gastrula epiblasts induced PSCs. Similar...
Recent studies have revealed an essential role for embryonic cortical development in the pathophysiology of neurodevelopmental disorders, including autism spectrum disorder (ASD). However, genetic basis and underlying mechanisms remain unclear. Here, we generate mutant human stem cell lines (Mut hESCs) carrying NR2F1-R112K mutation that has been identified a patient with ASD features investigate their alterations. Mut hESCs overproduce ventral telencephalic neuron progenitors (ventral NPCs)...
Direct neuronal reprogramming potentially provides valuable sources for cell-based therapies. Proneural gene Ascl1 converts astrocytes into induced (iN) cells efficiently both in vitro and vivo. However, the underlying mechanisms are largely unknown. By combining RNA sequencing chromatin immunoprecipitation followed by high-throughput sequencing, we found that expression of 1,501 genes was markedly changed during early stages Ascl1-induced astrocyte-to-neuron conversion regulatory regions...
Understanding of the molecular drivers lineage diversification and tissue patterning during primary germ layer development requires in-depth knowledge dynamic trajectories cell lineages across a series developmental stages gastrulation. Through computational modeling, we constructed at single-cell resolution, spatio-temporal transcriptome populations in germ-layers gastrula-stage mouse embryos. This atlas enables inference network activity underpinning specification differentiation...
The coactivator-associated arginine methyltransferase CARM1 catalyzes the methylation of histone H3 17/26 (H3R17/26me) and non-histone proteins at residues to regulate gene transactivation through profiling or Carm1 overexpression assays. However, direct relationship between H3R17/26me its causal role in mouse embryo development remains largely unclear. Here, we use rAPOBEC1-XTEN-Cas9n-UGI (BE3) efficiently introduce a point mutation (R17H) multiple Hist1/2H3 loci premature-stop codon into...
The ectoderm has the capability to generate epidermis and neuroectoderm plays imperative roles during early embryonic development. Our recent study uncovered a region with ectodermal progenitor potential in mouse embryo at day 7.0 revealed that Nodal inhibition is essential for its formation. Here, we demonstrate through brief of signaling vitro, stem cell (ESC)-derived epiblast cells (ESD-EpiSCs) could be committed transient populations, which possess ability give rise neural or epidermal...
Highlights•Self-renewing epiblast stem cells can be maintained under Nodal inhibition•Nodal-inhibited and the ectoderm display similar transcriptome•Blocking changes epigenome to that associated with potency•Nodal-inhibited differentiate preferentially ectodermal cellsSummaryThe molecular mechanism underpinning specification of ectoderm, a transient germ-layer tissue, during mouse gastrulation was examined here in cell-based model. We captured self-renewing cell population enhanced potency...
Clinical therapies of pluripotent stem cells (PSCs)-based transplantation have been hindered by frequent development teratomas or tumors in animal models and clinical patients. Therefore, clarifying the mechanism carcinogenesis cell therapy is great importance for reducing risk tumorigenicity. Here we differentiate Oct4-GFP mouse embryonic (mESCs) into neural progenitor (NPCs) find that a minority Oct4+ are continuously sustained at state. These can be enriched proliferated standard ESC...
Abstract Motivation Human gut microbiota plays a vital role in maintaining body health. The dysbiosis of is associated with variety diseases. It critical to uncover the associations between and disease states as well other intrinsic or environmental factors. However, inferring alterations individual microbial taxa based on relative abundance data likely leads false conflicting discoveries different studies. Moreover, effects underlying factors microbe–microbe interactions could lead...
Gastrulation is a key milestone in early mouse development when multipotent epiblast cells are allocated to progenitors of diverse tissue lineages that constitute the ensemble building blocks body plan. The analysis gene function revealed activity transcription factors likely be fundamental driving force underpinning lineage specification and patterning primary germ layers. developmental‐spatial transcriptome gastrulating embryo concerted interactive regulatory network anchored by...
Abstract The regulatory mechanisms governing cell fate determination, particularly lineage diversification during mammalian embryonic development, remain poorly understood with in-depth paradigms yet to be fully elucidated. Here, leveraging the epigenetic landscape of mouse gastrula, we identified p-Enh, a pre-marked enhancer in primitive streak region, as pivotal regulator for posterior tissue development embryos. Morphological and single-cell transcriptomic analyses confirmed lethality...
Abstract The first lineage allocation in mouse and human embryos separates the inner cell mass (ICM) from outer trophectoderm (TE). This symmetry breaking event is executed through polarization of cells at 8-cell stage subsequent asymmetric divisions, generating polar (TE) apolar cells. Here, we show that embryo unexpectedly asynchronous. Cells polarizing early late have distinct molecular morphological properties direct their following specification, with being biased towards producing TE...