A5045776835- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Cancer Diagnosis and Treatment
- Chromosomal and Genetic Variations
- Cancer Genomics and Diagnostics
- Tumors and Oncological Cases
- Cancer-related molecular mechanisms research
- COVID-19 diagnosis using AI
- Oral and Maxillofacial Pathology
- Nitrogen and Sulfur Effects on Brassica
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Air Quality and Health Impacts
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Immune cells in cancer
- Plant Molecular Biology Research
- Invertebrate Immune Response Mechanisms
- Diagnosis and Treatment of Venous Diseases
- COVID-19 impact on air quality
- Smoking Behavior and Cessation
- Phytase and its Applications
Zhejiang University
2014-2025
First Affiliated Hospital Zhejiang University
2022-2025
Bethune International Peace Hospital
2024
Shanghai Ocean University
2024
Renji Hospital
2023
Shanghai Jiao Tong University
2023
National Clinical Research
2023
University of Virginia
2023
Hong Kong Polytechnic University
2022
The University of Texas MD Anderson Cancer Center
2019-2021
Glioblastoma multiforme (GBM), the most aggressive brain cancer, recurs because glioblastoma stem cells (GSCs) are resistant to all standard therapies. We showed that GSCs, but not normal astrocytes, sensitive lysis by healthy allogeneic natural killer (NK) in vitro. Mass cytometry and single-cell RNA sequencing of primary tumor samples revealed GBM tumor-infiltrating NK acquired an altered phenotype associated with impaired lytic function relative matched peripheral blood from patients or...
Natural killer (NK)-cell recognition and function against NK-resistant cancers remain substantial barriers to the broad application of NK-cell immunotherapy. Potential solutions include bispecific engagers that target activity via an NK-activating receptor when simultaneously targeting a tumor-specific antigen, as well enhancing functionality using IL12/15/18 cytokine pre-activation.
Heterosis is a fundamental biological phenomenon characterized by the superior performance of hybrid compared with its parents. The underlying molecular basis for heterosis, particularly allopolyploids, remains elusive. In this study we analyzed transcriptomes Brassica napus parental lines and their F1 hybrids at three stages early flower development. Phenotypically, show remarkable heterosis in silique number grain yield. Transcriptome analysis revealed that various phytohormone (auxin...
Abstract Electronic cigarettes (e-cigarettes) generate an aerosol vapor (e-vapor) thought to represent a less risky alternative main stream smoke (MSS) of conventional tobacco cigarettes. RNA-seq analysis was used examine the transcriptomes differentiated human bronchial epithelial (HBE) cells exposed air, MSS from 1R5F reference cigarettes, and e-vapor with without added nicotine in vitro air-liquid interface model for cellular exposure. Our results indicate that while does not elicit many...
Summary Brassica napus ( AACC , 2 n = 38) is an important oilseed crop grown worldwide. However, little known about the population evolution of this species, genomic difference between its major genetic groups, such as European and Asian rapeseed, impacts historical large‐scale introgression events on young tetraploid. In study, we reported de novo assembly genome sequences rapeseed B. ), Ningyou 7, four progenitors compared these genomes with other available data from diverse cultivars. Our...
A wide range of RNA species interacting with microRNAs (miRNAs) form a complex gene regulation network and play vital roles in diverse biological processes. In this study, we performed genome-wide identification endogenous target mimics (eTMs) for miRNAs phased-siRNA-producing loci (PHAS) soybean focus on those involved lipid metabolism. The results showed that large number eTMs PHAS genes could be found soybean. Additionally, metabolism related were potentially regulated by 28 miRNAs, nine...
The role of respiratory tract microbes and the relationship between gut microbiomes in coronavirus disease 2019 (COVID-19) remain uncertain. Here, metagenomes sputum fecal samples from 66 patients with COVID-19 at three stages progression are sequenced. Respiratory tract, microbiome, peripheral blood mononuclear cell (PBMC) analyzed to compare microbiota intensive care unit (ICU) non-ICU (nICU) determine relationships microbiome immune response. In significantly fewer Streptococcus,...
The accurate and prompt diagnosis of infections is essential for improving patient outcomes preventing bacterial drug resistance. Host gene expression profiling as an approach to infection holds great potential in assisting early infection. To improve the precision diagnosis, we developed InfectDiagno, a rank-based ensemble machine learning algorithm via host patterns. Eleven data sets were used training method development, InfectDiagno was optimized by multi-cohort samples. Nine independent...
<title>Abstract</title> Recent advances in unbiased metagenomic next-generation sequencing (mNGS) enable simultaneous examination of microbial and host genetic material. In this study, we developed a multimodal machine learning-based diagnostic approach to differentiate lung cancer pulmonary infections using 402 bronchoalveolar lavage fluid (BALF) mNGS datasets. The training cohort revealed differences DNA/RNA composition, bacteriophage abundances, responses, including gene expression,...
Despite the epidemiological association between intrahepatic cholangiocarcinoma (ICC) and hepatitis B virus (HBV) infection, little is known about relevant oncogenic effects. A cohort of 32 HBV-infected ICC 89 non-HBV-ICC patients were characterized using whole-exome sequencing, proteomic analysis, single-cell RNA sequencing. Proteomic analysis revealed decreased cell-cell junction levels in HBV-ICC patients. The level had an inverse relationship with epithelial-mesenchymal transition (EMT)...
Synchronous multifocal tumors are common in the hepatobiliary and pancreatic system but because of similarities their histological features, oncologists have difficulty identifying precise tissue clonal origin through routine histopathological methods. To address this problem assist more diagnosis, we developed a computational approach for diagnosis based on naive Bayes algorithm (TOD‐Bayes) using ubiquitous RNA‐Seq data. Massive tissue‐specific data sets were first obtained from The Cancer...
Acute graft-vs.-host (GVHD) disease remains a common complication of allogeneic stem cell transplantation with very poor outcomes once the becomes steroid refractory. Mesenchymal cells (MSCs) represent promising therapeutic approach for treatment GVHD, but so far this strategy has had equivocal clinical efficacy. Therapies using MSCs require optimization taking advantage plasticity these in response to different microenvironments. In study, we aimed optimize cord blood tissue derived (CBti...
Gene expression profiling holds great potential as a new approach to histological diagnosis and precision medicine of cancers unknown primary (CUP). Batch effects different data types greatly decrease the predictive performance biomarker-based algorithms, few methods have been widely applied identify tissue origin CUP up now. To address this problem assist in more precise diagnosis, we developed gene rank-based majority vote algorithm for (TOD-CUP) most common cancer types. Based on massive...
Robust strategies to identify patients at high risk for tumor metastasis, such as those frequently observed in intrahepatic cholangiocarcinoma (ICC), remain limited. While gene/protein expression profiling holds great potential an approach cancer diagnosis and prognosis, previously developed protocols using multiple diagnostic signatures expression-based metastasis prediction have not been widely applied successfully because batch effects different data types greatly decreased the predictive...
Synchronous multifocal tumors often pose a diagnostic challenge for oncologists. The purpose of this study was to determine the clonal origin and metastatic relationship synchronous in hepatobiliary pancreatic system using multi-omic platforms. DNA samples were extracted from three masses harvested 50-year-old Han Chinese male patient who suffered tail, upper biliary duct, omentum at time diagnosis. these tested two platforms: next-generation sequencing (NGS) 390 key genes harboring...
The antibiotic resistome is the collection of all resistance genes (ARGs) present in an individual. Whether individual's susceptibility to infection and eventual severity coronavirus disease 2019 (COVID-19) influenced by their respiratory tract unknown. Additionally, whether a relationship exists between gut ARGs composition has not been fully explored. We recruited 66 patients with COVID-19 at three stages (admission, progression, recovery) conducted metagenome sequencing analysis 143...
Abstract Motivation Single-cell RNA sequencing (scRNA-seq) has enabled the characterization of different cell types in many tissues and tumor samples. Cell type identification is essential for single-cell profiling, currently transforming life sciences. Often, this achieved by searching combinations genes that have previously been implicated as being cell-type specific, an approach not quantitative does explicitly take advantage other scRNA-seq studies. Batch effects data platforms greatly...
ABSTRACT Glioblastoma, the most aggressive brain cancer, often recurs because glioblastoma stem cells (GSCs) are resistant to all standard therapies. Here, we show that patient-derived GSCs, but not normal astrocytes, highly sensitive lysis by healthy allogeneic natural killer (NK) in vitro . In contrast, single cell analysis of autologous, tissue infiltrating NK isolated from surgical samples high-grade patient tumors using mass cytometry and RNA sequencing revealed an abnormal phenotype...