A5057344007- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Reproductive Biology and Fertility
- Gynecological conditions and treatments
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Uterine Myomas and Treatments
- Ectopic Pregnancy Diagnosis and Management
- Pregnancy and Medication Impact
- Reproductive Health and Technologies
- Pregnancy and preeclampsia studies
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Intestinal and Peritoneal Adhesions
- Estrogen and related hormone effects
- Thyroid Disorders and Treatments
- Animal Genetics and Reproduction
- Minimally Invasive Surgical Techniques
- Growth Hormone and Insulin-like Growth Factors
- Ovarian cancer diagnosis and treatment
- Reproductive Health and Contraception
- Maternal and fetal healthcare
- Cardiac, Anesthesia and Surgical Outcomes
- Omental and Epiploic Conditions
- Urological Disorders and Treatments
Juntendo University
2016-2025
Tokuyama (Japan)
2018-2023
Nagasaki University Hospital
2019
Coventry (United Kingdom)
2012-2013
University of Warwick
2011-2012
University Hospital Coventry
2012
RELX Group (Netherlands)
2012
University of Siena
2012
Weatherford College
2009-2011
Tokyo Women's Medical University
2006
How has the treatment policy changed since introduction of health insurance coverage for assisted reproductive technology (ART) in Japan? This study was conducted as a nationwide fact-finding survey to determine current situation ART Japan, IZANAMI project. Among 616 facilities across 283 (45.9%) that responded first (December 2021-February 2022) and second surveys 2023-January 2024) were recruited. Information on clinical practices collected via questionnaire analyzed. with ≤200, 201-500,...
We evaluated the clinical efficacy of immunosuppressive treatment with tacrolimus for repeated implantation failure (RIF) patients who have elevated in T helper (Th1)/Th2 cytokine producing cell ratios.This was a prospective cohort study RIF (n = 42) peripheral blood Th1 (CD4(+) /IFN-γ(+) )/Th2 /IL-4(+) ) ratios at Sugiyama clinic between November 2011 and October 2013. Twenty-five were treated (treatment group) 17 received no (control group). Treatment group 2 days before embryo transfer...
During the human in vitro fertilization procedure assisted reproductive technology, intracytoplasmic sperm injection is routinely used to inject a spermatozoon or less mature elongating spermatid into oocyte. In some infertile men, round spermatids (haploid male germ cells that have completed meiosis) are most visible during testicular biopsy. The microsurgical of an oocyte as substitute commonly referred (ROSI). Currently, ROSI considered very inefficient and no clinical value. Herein, we...
Abstract Purpose To identify specific bacterial communities in vaginal and endometrial microbiotas as biomarkers of implantation failure by comprehensively analyzing their using next‐generation sequencing. Methods We investigated α‐ β‐diversities 16S rRNA gene sequencing compared profiles between 145 women with repeated (RIF) 21 controls who lacked the factors responsible for a high probability being healthy fertile to bacteria that induce failure. Results The had higher α‐diversities than...
We aimed to assess whether an imbalance of T-helper (Th) 1 and Th2 cells contributes implantation failure pregnancy loss.In this cross-sectional study, 197 consecutive patients with a history repeated (RIF) after three or more embryo transfer (ET) cycles and/or recurrent loss (RPL) two clinical losses underwent Th cell testing. After excluding 42 women aged ≥44 9 vitamin D supplementation, we recruited 146 including 79 RIF 81 RPL. Fourteen had both also 45 fertile 40 general infertile...
Abstract Purpose To clarify the mechanisms of intrauterine platelet‐rich plasma (PRP) infusion that support embryo implantation in vitro fertilization treatment. Methods Blood and endometrial samples were collected from four infertile women. Human stromal cells (HESCs) cultured passaged equally into cell culture dishes each patient. Two treated with PRP twice, other two vehicle. Subsequently, cultures without decidualized 8‐bromoadenosine 3′,5′‐cyclic AMP progesterone for 5 days. Results The...
The rate of oocyte decline follows a biphasic pattern, characterized by acceleration between 32 and 38 years old. Ovarian reserve is also affected external factors, including ovarian disease iatrogenic damage. aim this study was to histologically evaluate the impact endometriomas, laparoscopic cystectomy, age on follicle in healthy tissues surgically resected cyst walls.Sixty-one patients were found have endometriomas 42 non-endometriotic cysts. A small amount normal tissue obtained during...
Abstract The actions of glucocorticoids at the feto-maternal interface are not well understood. Here, we show that decidualization human endometrial stromal cells (HESCs) in response to progesterone and cAMP signaling is associated with a strong induction 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1) expression enzyme activity. Decidualization also triggered gradual decrease glucocorticoid receptor (GR) reciprocal increase mineralocorticoid (MR) levels. Gene profiling differentiating...
What is the prevalence of multiple pregnancy with zygotic splitting after single embryo transfer (SET)?The SET was 1.36%.In 2008, Japan Society Obstetrics and Gynaecology (JSOG) recommended adoption to reduce births. Since then, improve clinical rate, elective using blastocyst frozen-warmed ET has increased. Blastocyst culture zona pellucida manipulation, including ICSI AH, have been widely reported as risk factors for monozygotic twinning. However, all these studies may included cases...
Abstract Pregnancy critically depends on the transformation of human endometrium into a decidual matrix that controls embryo implantation and placenta formation, process driven foremost by differentiation polarization endometrial stromal cells mature senescent cells. Perturbations in underpin spectrum prevalent reproductive disorders, including failure early pregnancy loss, emphasizing need for new therapeutic interventions. Resveratrol is naturally occurring polyphenol, widely used its...
Abstract Background The aim of this study is to evaluate the relationship between chronic endometritis (CE) and a personalized window implantation (WOI), identified by results endometrial receptivity analysis (ERA), pregnancy outcomes following embryo transfer (ET) based on ERA outcomes. Methods single‐center, cross‐sectional was designed. population consisted 101 infertile women who underwent sampling June 2018 February 2020. We recruited 88 patients testing immunohistochemistry plasma cell...
Aim: Decidualization is essential for embryo implantation and placental development. We aimed to obtain transcriptome epigenome profiles primary endometrial stromal cells (ESCs) in vitro decidualized cells. Materials & methods: ESCs isolated from human tissues remained untreated (D0), or 4 days (D4) 8 (D8) the presence of 8-bromo-cAMP progesterone. Results: Among epigenetic modifications examined (DNA methylation, H3K27ac, H3K9me3 H3K27me3), H3K27ac patterns changed most dramatically, with a...
Upon breaching of the endometrial surface epithelium, implanting embryo embeds in decidualizing stroma. Retinoic acid (RA), a metabolite vitamin A, is an important morphogen during embryonic and fetal development, although role RA pathway surrounding decidual cells not understood. Here we show that transformation human stromal (HESCs) results profound reprogramming signaling metabolism pathways. Differentiating HESCs downregulate intracellular carrier proteins CRABP2 FABP5, responsible for...
Decidualizing human endometrial stromal cells (HESCs) profoundly up-regulate 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1), the enzyme that converts inert cortisone to active cortisol. We postulated induction of a cortisol gradient upon decidualization periimplantation endometrium may impact on uterine natural killer (uNK) cell population and local expression corticosteroid-dependent target genes.Midluteal biopsies (n = 55) were processed for uNK (CD56) analysis primary HESC cultures....
Problem We aim to investigate whether the peripheral blood T helper (Th) 1 cell level could predict pregnancy outcome in patients who have experienced repeated implantation failure ( RIF , three or more) after ART cycles. Method of study This is a prospective cohort total 124 women with showed elevated Th1/Th2 CD 4 + IFN ‐γ / IL ‐4 ) ratios (≥10.3) and received tacrolimus at Sugiyama Clinic between November 2011 July 2016. Patients were divided into groups as per Th1 levels: <22.8 Low;...
Abstract Problem We aimed to compare the therapeutic effects of hysteroscopic polypectomy with and without doxycycline treatment on chronic endometritis (CE) endometrial polyps. Method study: Design A cross‐sectional study was conducted 267 infertile patients, whom 243 were recruited, who underwent between March 2019 2020. During surgery, specimens for immunohistochemistry analysis plasma cell marker CD138 intrauterine bacterial culture obtained diagnose CE, prevalence CE analyzed. Of 222...
Sperm-specific phospholipase C-zeta (PLCζ) causes intracellular Ca2+ oscillations and thereby egg activation is accumulated into the formed pronucleus (PN) when expressed in mouse eggs by injection of cRNA encoding PLCζ, which consists four EF-hand domains (EF1-EF4) N terminus, X Y catalytic domains, C-terminal C2 domain. Those activities were analyzed expressing PLCζ mutants tagged with fluorescent protein Venus unfertilized or 1-cell embryos after fertilization. Nuclear localization signal...