A5100432115- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Mesenchymal stem cell research
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- DNA Repair Mechanisms
- Cancer Genomics and Diagnostics
- DNA and Nucleic Acid Chemistry
- Heat shock proteins research
- Cancer, Hypoxia, and Metabolism
- Hippo pathway signaling and YAP/TAZ
- Genomics, phytochemicals, and oxidative stress
- Advanced Glycation End Products research
- Angiogenesis and VEGF in Cancer
- Cancer therapeutics and mechanisms
- Chemical Synthesis and Analysis
- Multiple Myeloma Research and Treatments
- Kruppel-like factors research
- Ferroptosis and cancer prognosis
- PI3K/AKT/mTOR signaling in cancer
- Piperaceae Chemical and Biological Studies
- Redox biology and oxidative stress
First Affiliated Hospital of Fujian Medical University
2024-2025
Fujian Medical University
2024-2025
Nanjing Medical University
2025
Second Affiliated Hospital of Nanjing Medical University
2025
First Hospital of Jilin University
2025
Jilin University
2025
National Center for Advancing Translational Sciences
2020-2025
Beijing Tongren Hospital
2022-2024
Capital Medical University
2022-2024
Southern Medical University
2019-2022
Inducible heat shock protein 70 (Hsp70) is one of the most important HSPs for maintenance cell integrity during normal cellular growth as well pathophysiological conditions. Tumor necrosis factor receptor‐associated 6 (TRAF6) a crucial signaling transducer that regulates diverse array physiological and pathological processes essential activating NF‐κB pathway in response to bacterial lipopolysaccharide (LPS). Here we report novel mechanism Hsp70 preventing LPS‐induced activation RAW264.7...
Abstract Introduction Growing evidence has brought stem cell therapy to the forefront as new promising approaches towards stroke treatment. Of all candidate seeding cells, adipose-derived cells (ADSCs) are considered one of most appropriate for However, previous experimental data could not reach an agreement on efficacy ADSC transplantation treating in vivo well its mechanism which hinders their further clinical translational application. Methods To explore hADSC administration neurological...
Misexpression of chromatin modification factors and changed epigenetic modifications play crucial roles for tumorigenesis. Our previous studies demonstrated that inhibition enzymes EZH2, LSD1, DNMTs HDACs caused post-mitotic neuron-like differentiation in different cancer cells. However, how they regulate neuronal cells was unknown. Here, we show DNMT1 HDAC1 form interactions themselves, meanwhile, also interact with SMAD proteins β-CATENIN Chemical these leads to reduced level except HDAC1....
Nedd4 (Nedd4-1) is a Hect domain E3 ubiquitin ligase that also contains C2 and three WW domains. Despite numerous in vitro studies, its biological function vivo not well understood. Here we show disruption of Nedd4-1 mice (leaving Nedd4-2 intact) caused embryonic lethality at mid gestation, with pronounced heart defects (double-outlet right ventricle atrioventricular cushion defects) vasculature abnormalities. Quantitative mass spectrometry immunoblot analyses lysates from the wild type...
Dysregulation of the homeostatic balance histone H3 di- and tri-methyl lysine 27 (H3K27me2/3) levels caused by mis-sense mutation (H3K27M) is reported to be associated with various types cancers. In this study, we found that reduction in H3K27me2/3 H3.1K27M, a variants patients diffuse intrinsic pontine glioma (DIPG), dramatically attenuated presence 53BP1 (also known as TP53BP1) foci capability non-homologous end joining (NHEJ) human dermal fibroblasts. H3.1K27M mutant cells showed...
There are no effective therapies for advanced renal cell carcinoma (RCC), except VEGFR inhibitors with only ~50% response rate. To identify novel targets and biomarkers RCC is of great importance in treating RCC. In this study, we observed that eukaryotic initiation factor 3d (EIF3D) expression was significantly increased compared paracarcinoma tissue using immunohistochemistry staining western blot analysis. Furthermore, bioinformatics meta-analysis ONCOMINE microarray datasets showed EIF3D...
Abstract A decrease in oocyte developmental potential is a major obstacle for successful pregnancy women of advanced age. However, the age-related epigenetic modifications associated with dynamic transcriptome changes, particularly meiotic maturation-coupled mRNA clearance, have not been adequately characterized human oocytes. This study demonstrates decreased storage transcripts encoding key factors regulating maternal degradome fully grown oocytes similar defect maturation-triggered...
Most cases of gastric cancer (GC) are not diagnosed at early stage which can be curable, so it is necessary to identify effective biomarkers for its diagnosis and pre-warning. We have used methylated DNA immunoprecipitation (MeDIP) genes that frequently in cell lines. Promoter regions hypermethylation candidate were tested by methylation-specific polymerase chain reaction (MSP) serum samples, including GC (n=58), precancerous lesions (GPL, n=46), normal controls (NC, n=30). Eighty two...
Adipose-derived stem cells (ADSCs) have been shown to accelerate diabetic wound healing by promoting neovascularization, though the underlying mechanisms are not fully understood. This study aims explore whether ADSCs influence endothelial progenitor (EPCs) function enhance healing. Human adipose-derived (hADSCs) were isolated from patient adipose tissue and cultured under normal high glucose (HG) conditions. RNA sequencing analyzed gene expression, while immunofluorescence validated...
Abstract Colorectal cancer (CRC) is the second leading cause of cancer-related death worldwide, making exploration metastatic mechanisms crucial for therapeutic advancements. In this study, we identified receptor-interacting protein kinase 2 (RIPK2) as an independent risk factor poor CRC prognosis. Single-cell RNA sequencing and spatial transcriptomics revealed that a tumor cell cluster with high RIPK2 expression exhibited enhanced potential, closely linked to bacterial invasion. vitro in...
Abstract Multiple myeloma, a hematopoietic malignancy of terminally differentiated B cells, is the second most common hematological after leukemia. While patients have benefited from numerous advances in treatment recent years resulting significant increases to average survival time following diagnosis, myeloma remains incurable and relapse common. To help identify novel therapeutic agents with efficacy against disease search for biomarkers associated differential response treatment,...
We have used the Xenopus laevis egg extract system to study roles of vertebrate Dna2 in DNA replication and double-strand-break (DSB) repair. first establish that is a helicase, as well nuclease. further show nuclear protein actively recruited only after origin licensing. co-localizes foci with RPA found complex fork components And-1 Mcm10. interacts DSB repair checkpoint proteins Nbs1 ATM. also determine order arrival ATM, MRN, Dna2, TopBP1, duplex ends it same both S phase M extracts....
The molecular mechanisms underlying premature ovarian failure, which seriously impacts the physical and psychological health of patients, are not fully understood. Here, we present role TRDMT1 in reactive oxygen species-induced granulosa cells death, is considered an important cause failure. We found that species were increased a H2O2 dose-dependent manner accompanied by nuclear shuttling TRDMT1, DNA damage apoptosis cells. In addition, could be prevented antioxidant N-acetylcysteine or...
Hypoxic microenvironment and metabolic dysregulation of tumor impairs the therapeutic efficacy chemotherapeutic drugs, resulting in drug resistance metastasis, which has always been a challenge for treatment solid tumors, including renal cell carcinoma (RCC). Herein, starting from evaluation methionine metabolism RCC cells, we demonstrated that increased accumulation cells was mediated by L-type amino acid transporter 1 (LAT1) under hypoxia. Glutathione (GSH), as metabolite, would attenuate...
Ovarian cancer is a highly deadly disease, which often diagnosed at late stage with metastases. However, most ovarian cancers relapse after surgery combined platinum-based chemotherapy. Cancer stem cells (CSCs) are stem-like that possess high tumorigenic capability and display higher resistant against current therapies. our knowledge of CSCs their molecular mechanism remains sparse. In the study, we found KDM4C, histone demethylase, was required for cell (CSC) maintenance. Depletion KDM4C...
Abstract Non-small cell lung cancer (NSCLC) is one of the most malignant epithelial tumors. Studies have suggested that DNA hypermethylation promoters and abnormal histone modifications could induce tumor suppressor genes (TSGs) downregulation in NSCLC. However, exact mechanism TSGs remains unclear. In this study, we found there no difference regions Moreover, methylation region VILL promoter NSCLC compared with adjacent tissue samples by pyrosequencing. We further demonstrated was markedly...
Kazrin has recently been identified as a functional protein that is involved in cell-cell junctions and signal transduction. Here, we new isoform, F, which 518 aa length 97 unique at the N-terminus. Knockdown of F using siRNA caused cell apoptosis marked decrease viability measured by MTT TUNEL assays. Co-immunoprecipitation analysis revealed interacts with ARC (apoptosis repressor caspase recruitment domain) Bax (Bcl-2-associated X protein). Co-localization cytoplasm was determined...
Abstract p53 plays a pivotal role in maintaining the genomic stability of mouse embryonic stem cells (mESCs) through transcriptionally activating and repressing target genes. However, how recognizes its repressed targets remains largely unknown. Herein, we demonstrate that Sall4 negatively regulates DNA damage induced apoptosis (DIA) mESCs mediating recruitment to enhancers ESC-associated genes by from promoters p53-activated Upon damage, is an anti-apoptotic without altering activation....
DNA damage can occur naturally or through environmental factors, leading to mutations in replication and genomic instability cells. Normally, natural d-nucleotides were selected by polymerases. The template l-thymidine (l-T) has been shown be bypassed several types of However, fidelity nucleotide incorporation opposite vivo remains unknown. Here, we constructed plasmids containing a restriction enzyme (PstI) recognition site which the l-T lesion was site-specifically located within PstI...