A5047677716- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Cell death mechanisms and regulation
- Animal Virus Infections Studies
- Phagocytosis and Immune Regulation
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Immune Response and Inflammation
- Nuclear Receptors and Signaling
- Immune cells in cancer
- Bacillus and Francisella bacterial research
- Metabolism, Diabetes, and Cancer
- Cancer-related gene regulation
- CAR-T cell therapy research
- Macrophage Migration Inhibitory Factor
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Fungal Infections and Studies
- Monoclonal and Polyclonal Antibodies Research
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Multiple Myeloma Research and Treatments
- Ubiquitin and proteasome pathways
Agency for Science, Technology and Research
2015-2024
Singapore Immunology Network
2006-2024
Hohhot First Hospital
2023-2024
Bioprocessing Technology Institute
2011-2021
Changzhi Medical College
2019
Wuxi No.2 People's Hospital
2016
Institute of Molecular and Cell Biology
2005-2006
National University of Singapore
2002-2003
Background: Renal cell carcinoma (RCC) is a malignancy with poor prognosis. We aimed to explore whether the expression of Long Non-Coding RNA (LncRNA) growth arrest-specific transcript 5 (GAS5) associated RCC genesis. Methods: selected twelve clinical samples diagnosed for renal clear and found that LncRNA GAS5 levels were significantly reduced relative those in adjacent unaffected normal tissues. Results: In addition, was lower line A498 than HK-2. Furthermore, using functional cloning, we...
Apart from their killer identity, natural (NK) cells have integral roles in shaping the tumor microenvironment. Through immune gene deconvolution, present study revealed an interplay between NK and myeloid-derived suppressor (MDSCs) nonresponders of checkpoint therapy. Given that mechanisms governing outcome cell–to–myeloid cell interactions remain largely unknown, we sought to investigate cross-talk suppressive myeloid cells. Upon contact with tumor-experienced cells, monocytes neutrophils...
Dectin-1 is a C-type lectin that recognizes beta-glucan in the cell walls of fungi and plays an important role anti-fungal immunity. It signals via tyrosine kinase Syk adaptor protein Card9 to activate NF-kappaB leading proinflammatory cytokine production dendritic cells (DCs). Other than this, not much else known mechanism signaling. We demonstrate here stimulation DCs with zymosan triggers intracellular Ca2+ flux can be attenuated by blocking anti-Dectin-1 antibody or pre-treatment...
Toll-like receptor 3 (TLR3) mediates antiviral response by recognizing double-stranded RNA. Its cytoplasmic domain is tyrosine phosphorylated upon ligand binding and initiates downstream signaling via the adapter TIR-containing adaptor inducing interferon–β (TRIF). However, kinase responsible for TLR3 phosphorylation remains unknown. We show here that Bruton's (BTK)-deficient macrophages failed to secrete inflammatory cytokines IFN-β stimulation were impaired in clearing intracellular dengue...
Cell metabolism plays a pivotal role in regulating the effector functions of immune cells. Stimulatory cytokines, such as interleukin (IL)-2 or IL-12 and IL-15, activate glycolysis oxidative phosphorylation natural killer (NK) cells to support their enhanced functions. IL-10, pleiotropic cytokine, is known suppress macrophage activation but stimulate NK However, it remains unclear if IL-10 has an effect on human so, what metabolic mechanisms are affected, how these changes regulated...
Multiple Omicron sub-lineages have emerged, with XBB and XBB.1.5 subvariants becoming the dominant variants globally at time of this study. The key feature new is their ability to escape humoral immunity despite fact that there are limited genetic changes from preceding variants. This raises question whether should be regarded as a separate serotype viruses serologically clustered ancestral severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. Here, we present...
Lipid rafts are plasma membrane microdomains that enriched in cholesterol, glycosphingolipids, and glycosylphosphatidylinositol-anchored proteins play an important role the signaling of ITAM-bearing lymphocyte antigen receptors. Dectin-1 is a C-type lectin receptor (CLR) recognizes beta-glucan cell walls fungi triggers signal transduction via its cytoplasmic hemi-ITAM. However, it not known if similar to receptors, would also lipid integrity raft for physiological functions mediated by...
B cell-activating factor belonging to the TNF family (BAFF) and its receptor BAFF-R play critical roles in maturation survival of conventional peripheral cells. However, they appeared be dispensable for generation maintenance CD5(+) B-1 cells as BAFF(-/-) BAFF-R(-/-) mice have normal cell populations. Hence, it is presently unclear if are responsive BAFF regulates some aspects function. We show here that transmembrane activator CAML interactor (TACI) major receptors expressed by...
The ubiquitously expressed transmembrane adaptor Csk-binding protein (Cbp) recruits Csk to lipid rafts, where the latter exerts its negative regulatory effect on Src family of tyrosine kinases. We have inactivated Cbp in mouse germ line. In contrast gene inactivation, which leads embryonic lethality and impaired T-cell development, Cbp-deficient mice were viable exhibited normal development but with an increased thymocyte population. absence Cbp, amount that localizes rafts was greatly...
Invasive fungal infection is a serious health threat with high morbidity and mortality. Current antifungal drugs only demonstrate partial success in improving prognosis. Furthermore, mechanisms regulating host defense against pathogens remain elusive. Here, we report that the downstream of kinase 3 (Dok3) adaptor negatively regulates immunity neutrophils. Our data revealed Dok3 deficiency increased phagocytosis, proinflammatory cytokine production, netosis neutrophils, thereby enhancing...
The polycistronic mir-17–92 cluster, also known as oncomir-1, was previously shown to be essential for early B lymphopoiesis. However, its role in late-stage B-cell differentiation and function remains unexplored. Here we ablate mature cells demonstrate that is dispensable conventional development the periphery. Interestingly, mir-17–92-deficiency leads enhanced homing of plasma bone marrow during T-cell-dependent immune response selectively impairs IgG2c production. Mechanistically,...
The advent of the Omicron variant globally has hastened requirement for a booster vaccination dose to confer continuous protection against symptomatic SARS-CoV2 infection. However, different vaccines are available in countries, and individuals who had adverse reactions certain vaccine types require heterologous boosters. To understand efficacy regimens inducing humoral responses SARS-CoV2, we examined plasma antibodies frequencies RBD-specific B cells priming-booster regimens. We found that...
We compared the early total spike antibody (S-Ab) and neutralizing (N-Ab) responses to two vaccines.We studied 96 Pfizer 34 Sinovac vaccinees over a 14-month period from January 2021 February 2022. All received three doses of one type vaccine. Antibody levels (Roche Elecsys S-Ab Snibe N-Ab) were tested 10 days after first dose, 20 second booster dose.At all time points, mRNA vaccine generated higher N-Ab than inactivated virus (S-Ab: dose 2.48 vs. 0.4 BAU/mL, 2174 98 third 15,004 525 BAU/mL;...
T-cell-engaging bispecific antibodies (T-bsAbs) are promising immunotherapies for cancer treatment due to their capability of redirecting T-cells toward destroying tumor cells. Numerous T-bsAb formats have been developed, each with advantages and disadvantages in terms developability, immunogenicity, effector functions, pharmacokinetics. Here, we systematically compared T-bsAbs produced using eight different formats, evaluating the effect molecular design on manufacturability functionality....
Postmenopausal osteoporosis, a common type of osteoporosis in women, has become serious public health issue.Astragalus polysaccharides (APS), possessing various pharmacological activities, are the active ingredients Radix Astragali.It can be advantageous treatment postmenopausal osteoporosis.In present study, we evaluated potential therapeutic effects APS on by using mice model induced ovariectomy (OVX).Forty-eight female 6-week-old outbred ICR were randomly divided into six groups (N = 8):...
B lymphocytes undergo metabolic reprogramming upon activation to meet the bioenergetic demands for proliferation and differentiation. Yet, little is known if how fate of naive cells metabolically regulated. Here, we specifically delete von Hippel-Lindau protein (VHL) in using CD19-Cre demonstrate that balance essential cell survival. Loss VHL disturbs glycolytic oxidative causes severe reduction mature cells. Mechanistically, imbalance VHL-deficient cells, arising from over-stabilization...
Fas apoptosis inhibitory molecule (FAIM) has been demonstrated to confer resistance Fas-induced of lymphocytes and hepatocytes in vitro vivo. Here, we show that FAIM is up-regulated thymocytes upon T cell receptor (TCR) engagement faim(-/-) are highly susceptible TCR-mediated with increased activation caspase-8 -9. Furthermore, injection anti-CD3 antibodies leads augmented depletion CD4(+)CD8(+) cells the thymus mice compared wild-type control, suggesting plays a role thymocyte apoptosis....
Abstract Altered hepatic lipogenesis is associated with metabolic diseases such as obesity and hepatosteatosis. Insulin resistance compensatory hyperinsulinaemia are key drivers of these imbalances. Fas apoptosis inhibitory molecule (FAIM), a ubiquitously expressed antiapoptotic protein, functions mediator Akt signalling. Since acts at nodal point in insulin signalling, we hypothesize that FAIM may be involved energy metabolism. In the current study, C57BL/6 wild-type (WT) FAIM-knockout...
This study investigated the possible involvement of a specific caspase(s) (a family aspartate-specific cysteine proteases) in programmed cell death islet beta-cells due to sustained GTP depletion. Treatment (up 48 h) with 3 microg/ml mycophenolic acid (MPA), which specifically depletes intracellular guanine nucleotides, reduced cell-cycle progression from G1 phase into S and G2/M phases (as assessed by flow cytometry) and, subsequently, induced apoptosis HIT-15 cells (transformed pancreatic...