A5058260183- CAR-T cell therapy research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Electrospun Nanofibers in Biomedical Applications
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- 3D Printing in Biomedical Research
- Chemical Synthesis and Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Hydrogels: synthesis, properties, applications
- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Cancer Treatment and Pharmacology
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- Axon Guidance and Neuronal Signaling
- RNA modifications and cancer
- Nanowire Synthesis and Applications
- Immunotherapy and Immune Responses
- Wound Healing and Treatments
- Nerve injury and regeneration
University of North Carolina at Chapel Hill
2018-2025
North Carolina State University
2018-2025
UNC Lineberger Comprehensive Cancer Center
2018-2022
Harvard University
2008-2019
Harvard University Press
2019
University of Pittsburgh
2017
Inspire
2014
Howard Hughes Medical Institute
2008-2009
Methylation Mediation of cytosine bases, 5-methylcytosine (5mC), in DNA plays an important regulatory role mammalian genomes. patterns are often inherited across generations, but they can also be dynamic, suggesting that active demethylation pathways exist. One such pathway, best characterized plants, involves the removal 5mC base, and its replacement by C, via a repair mechanism. Kriaucionis Heintz (p. 929 , published online 16 April) now show that, as well genomes, there significant...
The DNA-templated polymerization of synthetic building blocks provides a potential route to the laboratory evolution sequence-defined polymers with structures and properties not necessarily limited those natural biopolymers. We previously reported efficient sequence-specific peptide nucleic acid (PNA) aldehydes. Here, we report enzyme-free, side-chain-functionalized PNA tetramer pentamer observed that single lysine-based side chain at various positions in block could proceed efficiently...
Cardiovascular disease is the leading cause of mortality worldwide. While reperfusion therapy vital for patient survival post-heart attack, it also causes further tissue injury, known as myocardial ischemia/reperfusion (I/R) injury in clinical practice. Exploring ways to attenuate I/R interest improving post-ischemic recovery. A platelet-inspired nanocell (PINC) that incorporates both prostaglandin E2 (PGE2)-modified platelet membrane and cardiac stromal cell-secreted factors target heart...
Significance Drug delivery depots used in the clinic today are single use, with no ability to refill once exhausted of drug. Our system exploits nucleic acid complementarity drug-delivering through blood. The utility this approach is demonstrated by its inhibit tumor growth a greater extent than strategies that rely on enhanced permeability and retention alone. We anticipate our will be directly applicable therapies for many diseases, including cancer, wound healing, inflammation, drug...
Tissue reinnervation following trauma, disease, or transplantation often presents a significant challenge. Here, we show that the delivery of vascular endothelial growth factor (VEGF) from alginate hydrogels ameliorates loss skeletal muscle innervation after ischemic injury by promoting both maintenance and regrowth damaged axons in mice. Nerve (NGF) glial-derived neurotrophic (GDNF) mediated VEGF-induced axonal regeneration, expression is induced VEGF presentation. Using vitro vivo modeling...
Diabetic foot ulcers (DFU) are a major, debilitating complication of diabetes mellitus. Unfortunately, many DFUs refractory to existing treatments and frequently lead amputation. The development more effective therapies has been hampered by the lack predictive in vitro methods investigate mechanisms underlying impaired healing. To address this need for realistic wound-healing models, we established patient-derived fibroblasts from site-matched controls used them construct three-dimensional...
Dogs are becoming an important model for human cancers, and successfully troubleshooting issues with genetically modified T cell immunotherapy round solid neoplasms in dogs provides a unique opportunity to improve efficacy, safety, affordability humans as well. Unfortunately, activation optimal viral transduction has not been determined, restricting advancements canine immunotherapy. Two αCD3 two αCD28 antibody clones stimulation have described the literature, but no studies undertaken...
Targeting small molecules to diseased tissues as therapy or diagnosis is a significant challenge in drug delivery. Drug-eluting devices implanted during invasive surgery allow the controlled presentation of drugs at disease site, but cannot be modified once complete. We demonstrate that bioorthogonal click chemistry can used target circulating hydrogels resident intramuscularly tissues. also repeatedly targeted area over course least one month. Finally, two reactions were segregate injected...
Dry biomaterial scaffolds (Drydux) are a novel platform that enhances viral transduction ~10-fold while simplifying therapeutic cell production. Here, we systematically evaluate the properties governing enhancement.
Abstract Chimeric antigen receptor T (CAR‐T) cell therapy has produced impressive clinical responses in patients with B‐cell malignancies. Critical to the success of CAR‐T therapies is achievement robust gene transfer into cells mediated by viral vectors such as gamma‐retroviral vectors. However, current methodologies retroviral rely on spinoculation and use retronectin, which may limit implementation cost‐effective therapies. Herein, a low‐cost, tunable, macroporous, alginate scaffold that...
Human embryonic and induced pluripotent stem cells (hESC/hiPSC) are promising cell sources for the derivation of large numbers specific types tissue engineering therapy applications. We have describe a directed differentiation protocol that generates fibroblasts from both hESC hiPSC (EDK/iPDK) support repair regeneration epithelial in engineered, 3D skin equivalents. In current study, we analyzed secretory profiles EDK iPDK to investigate production factors activate promote angiogenesis....
Developing the next generation of cellular therapies will depend on fast, versatile, and efficient reprogramming. Novel biomaterials play a central role in this process by providing scaffolding bioactive signals that shape cell fate function. Previously, our lab reported dry macroporous alginate scaffolds mediate retroviral transduction primary T cells with efficiencies rival gold-standard clinical spinoculation procedures, which involve centrifugation Retronectin-coated plates. This...