A5034454190- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- interferon and immune responses
- Immune Response and Inflammation
- Mitochondrial Function and Pathology
- Reproductive Biology and Fertility
- T-cell and B-cell Immunology
- NF-κB Signaling Pathways
- Hernia repair and management
- Sperm and Testicular Function
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Skin and Cellular Biology Research
- Immunodeficiency and Autoimmune Disorders
- Stress Responses and Cortisol
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Effects of Environmental Stressors on Livestock
- Ovarian function and disorders
- Animal Genetics and Reproduction
- Eosinophilic Disorders and Syndromes
- Metabolomics and Mass Spectrometry Studies
- Pelvic and Acetabular Injuries
- Birth, Development, and Health
- Growth Hormone and Insulin-like Growth Factors
University of Veterinary Medicine Vienna
2014-2024
BOKU University
2012-2024
Universitätsklinikum Tulln
2015-2021
Landwirtschaftliche Fachschule Tulln
2003-2021
Medical University of Graz
2020
Maastricht University Medical Centre
2011
University of Vienna
2006-2008
Consejo Superior de Investigaciones Científicas
2005
Centro Nacional de Biotecnología
2005
Ludwig Boltzmann Institute for Cancer Research
2005
Abstract Spermatozoa harbour a complex and environment-sensitive pool of small non-coding RNAs (sncRNAs) 1 , which influences offspring development adult phenotypes 1–7 . Whether spermatozoa in the epididymis are directly susceptible to environmental cues is not fully understood 8 Here we used two distinct paradigms preconception acute high-fat diet dissect epididymal versus testicular contributions sperm sncRNA health. We show that spermatozoa, but developing germ cells, sensitive...
The dynamics by which mitochondrial DNA (mtDNA) evolves within organisms are still poorly understood, despite the fact that inheritance and proliferation of mutated mtDNA cause fatal incurable diseases. When two haplotypes present in a cell, it is usually assumed segregation (the one haplotype over another) negligible. We challenge this assumption showing depends on genetic distance between haplotypes. provide evidence creating four mouse models containing pairs varying diversity. find...
Dangerous damage to mitochondrial DNA (mtDNA) can be ameliorated during mammalian development through a highly debated mechanism called the mtDNA bottleneck. Uncertainty surrounding this process limits our ability address inherited diseases. We produce new, physically motivated, generalisable theoretical model for populations development, allowing first statistical comparison of proposed bottleneck mechanisms. Using approximate Bayesian computation and mouse data, we find most support...
Abstract Type I IFNs (IFN-α/β) modulate innate immune responses. Here we show activation of transcription factor IFN regulatory 3, the synthesis large amounts IFN-β mRNA, and type signal transduction in macrophages infected with Listeria monocytogenes. Expression bacterial virulence protein listeriolysin O was necessary, but not sufficient, for efficient production. Signaling through a pathway involving receptor Stat1 sensitized to L. monocytogenes-induced cell death manner requiring...
STAT5B is often mutated in hematopoietic malignancies. The most frequent mutation, Asp642His (N642H), has been found over 90 leukemia and lymphoma patients. Here, we used the Vav1 promoter to generate transgenic mouse models that expressed either human or STAT5BN642H compartment. While STAT5B-expressing mice lacked a phenotype, STAT5BN642H-expressing rapidly developed T cell neoplasms. Neoplasia manifested as transplantable CD8+ leukemia, indicating mutation drives cancer development....
Vital mitochondrial DNA (mtDNA) populations exist in cells and may consist of heteroplasmic mixtures mtDNA types. The evolution these through development, ageing, generations is central to genetic diseases, but poorly understood mammals. Here we dissect population dynamics using a dataset unprecedented size temporal span, comprising 1947 single-cell oocyte 899 somatic measurements heteroplasmy change throughout lifetimes two genetically distinct mouse models. We provide novel detailed...
Abstract Objective STAT proteins play an important role in cytokine signaling. Some investigators have reported preferential activation of STAT‐1, and others STAT‐3, response to endogenous interleukin‐6 (IL‐6), patients with rheumatoid arthritis. The present study was undertaken investigate synovial STAT‐1 STAT‐3 experimental animal model Methods Zymosan injected intraarticularly into naive wild‐type (WT), IL‐6 −/− , mice induce Western blots lysates were probed phosphospecific antibodies...
Transcriptional pathways controlling the development of CD44 hi memory phenotype (MP) T cells with “innate-like” functions are not well understood. Here we show that BTB (bric-a-brac, tramtrack, broad complex) domain-containing protein promyelocytic leukemia zinc finger (PLZF) is expressed in , but lo CD4 + cells. Transgenic expression PLZF during cell and CD8 induced a intrinsic program leading to an increase peripheral MP corresponding decrease naïve The produced IFNγ upon PMA/ionomycin...
Tyrosine kinase 2 (TYK2) is a member of the Janus (JAK) family and involved in cytokine signalling. In vitro analyses suggest that TYK2 also has kinase-independent, i.e., non-canonical, functions. We have generated gene-targeted mice harbouring mutation ATP-binding pocket domain. The Tyk2 kinase-inactive (Tyk2K923E) are viable show no gross abnormalities. kinase-active required for full-fledged type I interferon- (IFN) induced activation transcription factors STAT1-4 vivo antiviral defence...
Clonal cultures of Histomonas meleagridis, Tetratrichomonas gallinarum and a Blastocystis sp. were established for the first time. Single microbes successfully isolated from mixture micro-organisms obtained caecal contents turkeys, using micromanipulation approach. The cloned parasites propagated in vitro maintained through continuous passages multiplying to high numbers. Identification protists was done by morphological investigation identifying various forms each parasite. PCR partial...
Perlecan is a heparansulfate proteoglycan found in basement membranes, cartilage, and several mesenchymal tissues that form during development, tumour growth, tissue repair. Loss-of-function mutations the perlecan gene mice are associated with embryonic lethality caused primarily by cardiac abnormalities probably due to hemopericards. The aim of present study was investigate mechanism underlying early pathophysiological relevance for heart function. Perlecan-deficient murine stem cells were...
Recurrent gain-of-function mutations in the transcription factors STAT5A and much more STAT5B were found hematopoietic malignancies with highest proportion mature T- natural killer-cell neoplasms (peripheral T-cell lymphoma, PTCL). No targeted therapy exists for these heterogeneous often aggressive diseases. Given shortage of models PTCL, we mimicked graded or activity by expressing hyperactive Stat5a variants at low high levels system transgenic mice. Only mice developed a lethal disease...
Abstract We have recently reported that tyrosine kinase 2 (Tyk2)-deficient mice a selective defect in the vivo defense against certain viruses. In our current study we show Tyk2 is essential for murine CMV (MCMV). challenges with MCMV revealed impaired clearance of virus from organs and decreased survival absence Tyk2. Our vitro studies demonstrate replicates to dramatically higher titers Tyk2-deficient macrophages compared wild-type cells. an role type I IFN (IFN-αβ) control replication,...