A5026872246- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- dental development and anomalies
- Cancer-related molecular mechanisms research
- Periodontal Regeneration and Treatments
- Oral and gingival health research
- Bone and Dental Protein Studies
- Hepatitis B Virus Studies
- Dental Trauma and Treatments
- Dental Implant Techniques and Outcomes
- MicroRNA in disease regulation
- Prostate Cancer Treatment and Research
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- RNA Research and Splicing
- Connective tissue disorders research
- Viral Infections and Outbreaks Research
- Orthopaedic implants and arthroplasty
- Innovations in Medical Education
- Infection Control in Healthcare
- Hepatitis Viruses Studies and Epidemiology
- Bone health and treatments
- Dental materials and restorations
- Healthcare Regulation
Universidade Federal do Espírito Santo
2014-2023
Universidade Estadual de Campinas (UNICAMP)
2016-2022
University of Birmingham
2022
Periodontal ligament cells (PDLCs) have well documented osteogenic potential; however, this commitment can be highly heterogenous, limiting their applications in tissue regeneration. In study, we use PDLC populations characterized by high and low potential (h-PDLCs l-PDLCs, respectively) to identify possible sources of such heterogeneity investigate whether the differentiation enhanced epigenetic modulation. h-PDLCs, basal expression levels pluripotency markers (NANOG, OCT4), DNA...
Human bone marrow-derived mesenchymal stem cells (hBMSCs) are important for tissue regeneration but their epigenetic regulation is not well understood. Here we investigate the ability of a non-nucleoside DNA methylation inhibitor, RG108 to induce changes at both global and gene-specific levels in order enhance cell markers, hBMSCs. hBMSCs were treated with complete culture medium, 50 μM DMSO three days subjected viability apoptosis assays, methylation/hydroxymethylation, transcript levels'...
Aim: This study aimed to evaluate the ability of human periodontal ligament stem cells (PDLSCs) with high (HP-PDLSCs) and low (LP-PDLSCs) osteogenic potential, in addition mixed cells, repair bone tissue. Methods: Cell phenotype, proliferation differentiation were evaluated. Undifferentiated PDLSCs injected into rat calvarial defects new was evaluated by μCT, histology real-time PCR. Results: exhibited a typical mesenchymal cell phenotype HP-PDLSCs showed lower proliferative higher potential...
Periodontal ligament stem cells (PDLCs) can be used as a valuable source in cell therapies to regenerate bone tissue. However, the potential therapeutic outcomes are unpredictable due PDLCs’ heterogeneity regarding capacity for osteoblast differentiation and mineral nodules production. Here, we identify epigenetic (DNA (hydroxy)methylation), chromatin (ATAC-seq) transcriptional (RNA-seq) differences between PDLCs presenting with low (l) high (h) osteogenic potential. The primary populations...
Mesenchymal cells' biology has been an important investigative tool to maximize bone regeneration through tissue engineering. Here we used mesenchymal cells from periodontal ligament (PDLCs) with high (h-) and low (l-) osteogenic potential, isolated different donors, investigate the impact of individual epigenetic transcriptional profiles on potential.Genome-wide gene-specific DNA (hydroxy) methylation, mRNA expression immunofluorescence analysis were carried out in h- l-PDLCs at DMEM...
A iniciação científica é uma oportunidade aos acadêmicos de desenvolver senso crítico, ético e profissional, com o intuito aumentar a evidenciação conduzir que melhor prática odontológica pode oferecer. universidade um cenário importante estímulo incentivo à pesquisa, tendo papel fundamental no desenvolvimento desta atividade, sendo necessário apoio institucional professores interessados. O objetivo conhecer interesse dos graduandos do curso Odontologia da Universidade Federal Espírito Santo...
ABSTRACT The aim this study is to present a clinical case of conservative aesthetic restorative treatment teeth 11 and 21 in patient diagnosed with molar incisor hypomineralization. An 18-year-old female came the Outpatient Restorative Dentistry Clinic at Federal University Espírito Santo, complaining mainly not feeling comfortable color change her anterior teeth. Molar hypomineralization was molars incisors negative psychosocial implications for aesthetics involved were noticed. Due factors...
Chromatin conformation, DNA methylation pattern, transcriptional profile, and non-coding RNAs (ncRNAs) interactions constitute an epigenetic pattern that influences the cellular phenotypic commitment impacts clinical outcomes in regenerative therapies. Here, we investigated landscape of SP7 transcriptor factor (SP7) Distal-Less Homeobox 4 (DLX4) osteoblastic transcription factors (TFs), human periodontal ligament mesenchymal cells (PDLCs) with low (l-PDLCs) high (h-PDLCs) osteogenic...
Objetivo: O objetivo do estudo foi avaliar o conhecimento sobre a vacinação da hepatite B, de acadêmicos dos cursos Medicina, Odontologia e Enfermagem Universidade Federal Espírito Santo (UFES). Materiais Métodos: A metodologia utilizada descritiva transversal com abordagem quantitativae qualitativa, através questionário semiestruturado validado em uma amostra 298 acadêmicos. Os dados quantitativos foram expressos frequências absolutas relativas, aos qualitativos seguiu-se análise Conteúdo...
115 (84,56%) de Medicina e 62 (76,55%) Odontologia são vacinados contra o tétano.Conclusão: O conhecimento dos acadêmicos sobre a vacina antitetânica foi satisfatório, no entanto, nem todos estavam vacinados.
Imunização; Vacinação; Riscos ocupacionais; Hepatite B; Tétano.
Here, we evaluated whether the histone lysine demethylase 5B (JARID1B), is involved in osteogenic phenotype commitment of periodontal ligament cells (PDLCs), by considering their heterogeneity for osteoblast differentiation.Epigenetic, transcriptional, and protein levels a gene set, osteogenesis, were investigated performing genome-wide DNA (hydroxy)methylation, mRNA expression, western blotting analysis at basal (without induction), 3rd 10th days stimulus, vitro, using PDLCs with low (l)...