A5062197243- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Infective Endocarditis Diagnosis and Management
- Reproductive tract infections research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Genomics and Phylogenetic Studies
- Influenza Virus Research Studies
- Streptococcal Infections and Treatments
- Virology and Viral Diseases
- Yersinia bacterium, plague, ectoparasites research
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Long-Term Effects of COVID-19
- Syphilis Diagnosis and Treatment
- Sinusitis and nasal conditions
- Acute Kidney Injury Research
- SARS-CoV-2 detection and testing
- Amoebic Infections and Treatments
- Liver physiology and pathology
- Chronic Kidney Disease and Diabetes
- COVID-19 epidemiological studies
- Coccidia and coccidiosis research
- COVID-19 and healthcare impacts
Istituto Superiore di Sanità
2015-2024
University of Pisa
2024
Ospedale Policlinico San Martino
2020
University of Genoa
2020
Agenzia per la Promozione All'estero e l'Internazionalizzazione Delle Imprese Italiane
2017
Mylan (South Africa)
2017
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2017
University of Florence
2017
University of Siena
2017
Azienda Ospedaliero-Universitaria Careggi
2017
Abstract Background The prevalence of kidney involvement during SARS-CoV-2 infection has been reported to be high. Nevertheless, data are lacking about the determinants acute injury (AKI) and combined effect chronic disease (CKD) AKI in COVID-19 patients. Methods We collected on patient demographics, comorbidities, medications, vital signs, baseline laboratory test results in-hospital treatment patients with consecutively admitted our Institution. Chronic was defined as eGFR < 60 mL/min...
A seroprevalence study of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was conducted in a high-incidence area located northeastern Italy.All citizens above 10 years age resident five municipalities the Autonomous Province Trento, with highest incidence disease 2019 (COVID-19) cases, were invited to participate study. Among 6098 participants, 6075 sera and standardized questionnaire administered face-to-face collected between 5 May 15 2020 examined. Symptomatic individuals...
We report an increase of serogroup C Neisseria meningitidis invasive meningococcal disease in Tuscany. From January 2015 to end February 2016, 43 cases were reported, among which 10 fatal, compared two caused by recorded 2014 and three 2013. No secondary occurred. Thirty–five strains belonged C:P1.5–1,10–8:F3–6:ST-11(cc11). Control measures have been adopted immunisation campaigns implemented. Studies on risk factors carriage are ongoing.
The aim of this study was to evaluate the antimicrobial susceptibilities 866 Neisseria meningitidis invasive strains during 11 years surveillance in Italy. Two and six were resistant ciprofloxacin rifampin, respectively. Forty-five percent penicillin intermediate, associated with hypervirulent serogroup C clonal complex 11. All susceptible cephalosporins.
The sexually transmitted infection gonorrhea remains a public health concern for becoming resistant to drug treatments available. purpose of this study was evaluate the usefulness matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) identify and cluster Neisseria gonorrhoeae. From current monitoring in Italy, as part European Gonococcal Antimicrobial Surveillance Programme (EURO-GASP), 93 gonococci collected from 2007 2012 susceptible (44 isolates) (49...
We conducted a study to determine the main traits of pertussis among unimmunized infants less than 6 months age. From August 2012 March 2015, 141 nasopharyngeal aspirates (NPAs) were collected from with respiratory symptoms attending 2 major hospitals in Rome. Clinical data recorded and analyzed. Lab-confirmation was performed by culture realtime PCR. B. virulence-associated genes (ptxP, ptxA prn), together multilocus variable-number tandem repeat analysis (MLVA), also investigated...
ObjectivesTo describe a spatio-temporal cluster of invasive meningococcal disease (IMD) due to serogroup C meningococci, occurred in restricted area Tuscany between January and October 2015, the results whole genome sequencing (WGS).MethodsSurveillance activities public health measures were implemented Region. Bacterial isolates from IMD cases characterized by National Reference Laboratory Istituto Superiore di Sanità (ISS), WGS was performed on available strains. The kSNP software used...
In Tuscany, Italy, where a universal immunization program with monovalent meningococcal C conjugate vaccine (MCC) was introduced in 2005, an outbreak of invasive disease (IMD) due to the hypervirulent strain Neisseria meningitidis C/cc11 occurred 2015–2016, leading reactive campaign using either tetravalent (ACWY) or MCC vaccine. During outbreak, IMD serogroup (MenC) cases were also reported among vaccinated individuals. This study aimed characterize vaccines (MenC-vaccines) failures and...
Abstract Four cases of infection with serogroup X meningococci (MenX) (1 in 2015 and 3 2016) occurred migrants living refugee camps or reception centers Italy. All MenX isolates were identified as clonal complex 181. Our report suggests that represents an emerging health threat for persons arriving from African countries.
The aim was to investigate an outbreak of invasive meningococcal disease (IMD) in Southern Sardinia.Epidemiological and microbiological investigations were performed. latter included antimicrobial susceptibility testing whole-genome sequencing (WGS).Seven individuals with severe IMD found be infected serogroup B (MenB) Neisseria meningitidis the first quarter 2018. Five seven cases (five males; mean age 19 years; range 18-21 CFR 40%) due a unique strain B:P1.5-1,10-8:F3-6:ST-11(cc11),...
Background Following the introduction of meningococcal serogroup C conjugate vaccine in Italy 2005, changes epidemiology Invasive Meningococcal Disease (IMD) were expected. The study aims to describe epidemiological trend and characterize isolates collected during period 2008/09-2012/13 by multilocus sequence typing (MLST). Data on laboratory confirmed diseases from National Surveillance System IMD reported. Methods Poisson regression models used estimate incidence rate over time....
The four-component meningococcal serogroup B vaccine (4CMenB) contains antigens present in the majority of meningococci causing invasive disease (IMD) and may potentially offer protection against strains belonging to non-B serogroups.This study aimed evaluate ability 4CMenB-induced antibodies kill, a human serum bactericidal assay (hSBA), main genotypes responsible for IMD Italy.Meningococci, collected between 2015 2017, was characterized PorA, FetA sequence type, clonal complex. Twenty...
To evaluate the decline of antibodies induced by SARS-CoV-2 infection, individuals resident in 5 municipalities Autonomous Province Trento, Northern Italy, who resulted IgG positive for anti-SARS-CoV-2 nucleocapsid (NC) May 2020, were tested four months later.Anti-SARS-CoV-2 NC detected using Abbott assay. Samples that gave a negative result re-tested Liaison assay to assess anti-spike (S) S1/S2 antibodies. The fifty-percent tissue culture infective dose (TCID50) neutralizing was performed...
In Italy, B and C are the predominant serogroups among meningococci causing invasive diseases. Nevertheless, in period from 2013 to 2016, an increase serogroup W Neisseria meningitidis (MenW) was observed. This study intends define main characteristics of 63 MenW isolates responsible meningococcal disease (IMD) Italy 2000 2016. We performed whole genome sequencing on bacterial or single gene culture-negative samples evaluate molecular heterogeneity. Our finding cocirculation Hajj South...
In Italy, the incidence of invasive meningococcal disease (IMD) has remained stable since 2007 (around 0.3 cases/100,000 inhabitants). However, as reported for other European countries, an increase serogroup Y Neisseria meningitidis been observed. this study we report IMD cases from to 2013 in Italy and investigate clinical epidemiological features affected by Y. Molecular characteristics strains are also described. During period, proportion due increased, ranging 2% 17% (odds ratio (OR):...
In the last decade, incidence of invasive meningococcal disease (IMD) in Croatia remained stable at approximately 1 case per 100 000 inhabitants, affecting mainly children aged ≤5 years. We report molecular characterization meningococci causing IMD occurring from June 2009 to January 2014 Croatia. Genomic DNA 50 clinical isolates was analysed for serogroup, multilocus sequence typing and allele type two outer membrane protein genes, porA iron-regulated fetA. Furthermore, 22 them were...
A strictly defined subset of gonococci (n = 65) isolated in Italy from 2011 to 2014 was characterized by antimicrobial susceptibility for cefixime (CFM) and ceftriaxone (CRO) sequencing resistance determinant genes (penA, mtrR, porB1b, ponA) extended-spectrum cephalosporins Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST). The penA mosaic alleles XXXIV XXXV were found all resistant (R) decreased (DS) CFM, except one. They associated with an adenine deletion the mtrR promoter plus...
The emergence of antibiotic resistant strains poses a great concern for gonorrhea treatment. aim this study was to characterize penicillinase-producing Neisseria gonorrhoeae (PPNG) isolates collected in Italy two time frames, 2003-2004 and 2014-2015. A total 80 PPNG were characterized the blaTEM gene variant plasmid type. Furthermore, gonococci typed using multiantigen sequence typing. Antibiotic susceptibility assay performed penicillin, ciprofloxacin, ceftriaxone, spectinomycin by Etest...
Neisseria meningitidis is one of the few commensal bacteria that can even cause large epidemics invasive meningococcal disease (IMD). N. meningitis serogroup C belonging to hypervirulent clonal complex 11 (cc11) represents an important public health threat worldwide. We reconstructed dispersal patterns strains C:cc11 by phylogenomic time trees.
In recent decades, Neisseria gonorrhoeae has developed resistance to several antimicrobial classes. Molecular epidemiology approaches are useful for detecting emerging, often resistant, gonococcal clones. this study, 67 N. isolates from different anatomic sites, collected over 8 years in Italy, were analyzed by whole genome sequencing (WGS). WGS was performed using the Illumina NextSeq 500 platform. Phylogenetic analysis based on core single nucleotide polymorphism (SNP) and multilocus...
We report pertussis cases in 4 infants less than 6 months admitted with symptoms compatible to the intensive care unit of Università Cattolica del Sacro Cuore Rome, April 2014. Realtime PCR confirmed diagnosis for infants, 2 them were cousins, and household contacts 1 them. Analysis toxin, its promoter pertactin was also performed. First all, this emphasizes need investigate contact pertussis; secondly, evaluate selective vaccination members newborns as an effective program reduce infants.
Background Typing of Neisseria meningitidis isolates is crucial for the surveillance invasive meningococcal disease (IMD). We performed a molecular epidemiology study N . serogroup B (MenB) causing IMD in Italy between 2014 and 2017 to describe circulating strains belonging this serogroup, with particular regards two factor H-binding protein (FHbp) subfamilies present bivalent MenB vaccine. Materials methods A total 109 culture positive 46 negative samples were collected within National...
Enhanced laboratory-based surveillance of invasive meningococcal disease (IMD) in Italy was only assessed indirectly by numerically comparing data cases with hospital discharge records (HDR). In this study, we evaluated the completeness, timeliness and sensitivity IMD from 2015 to 2018. Completeness were described at national subnational level. A capture-recapture analysis conducted evaluate positive predictive value (PPV) using HDR as external source a combination deterministic...