A5071656979- Medical Imaging and Pathology Studies
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Radiopharmaceutical Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Magnetism in coordination complexes
- Metal complexes synthesis and properties
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- CAR-T cell therapy research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Metal-Organic Frameworks: Synthesis and Applications
- Medical Imaging Techniques and Applications
- Phagocytosis and Immune Regulation
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Zeolite Catalysis and Synthesis
- Immune cells in cancer
- HER2/EGFR in Cancer Research
- Immune Cell Function and Interaction
- Lanthanide and Transition Metal Complexes
University of California, Los Angeles
2021-2025
Institute for Molecular Medicine
2024
UCLA Jonsson Comprehensive Cancer Center
2024
Amgen (United States)
2015-2019
Washington University in St. Louis
2012-2014
Mallinckrodt (United States)
2011-2013
Syracuse University
2007-2010
University of Canterbury
2007
Universitat de València
2007
Purpose: Talimogene laherparepvec, a new oncolytic immunotherapy, has been recently approved for the treatment of melanoma. Using murine version virus, we characterized local and systemic antitumor immune responses driving efficacy in syngeneic models.Experimental Design: The activity talimogene laherparepvec was against melanoma cell lines using an vitro viability assay. Efficacy OncoVEXmGM-CSF (talimogene with mouse granulocyte-macrophage colony-stimulating factor transgene) alone or...
Pertuzumab is a monoclonal antibody that binds to HER2 and used in combination with another HER2-specific antibody, trastuzumab, for the treatment of HER2+ metastatic breast cancer. an binding site distinct from its affinity enhanced when trastuzumab present. We aim exploit this pertuzumab epitope adapt as PET imaging agent by radiolabeling (89)Zr increase sensitivity detection vivo. Here, we investigate biodistribution (89)Zr-pertuzumab HER2-expressing BT-474 HER2-nonexpressing MDA-MB-231...
The reaction in water of Cu(NO3)2·2.5H2O with 2,2′-bipyridine (bipy), 1,10-phenanthroline (phen), or 1,10-phenanthroline-5-amine (phenam), and sodium pyrophosphate (Na4P2O7), at various pHs, afforded three new copper(II)-pyrophosphate complexes, namely, {[Cu(bipy)(cis-H2P2O7)]2}·3H2O (1a), {[Cu(phen)(H2O)]4(HP2O7)2}(ClO4)2·4H2O (2), {[Cu2(phenam)2(P2O7)]2·25H2O}n (3). A solvent free crystalline phase 1a was also isolated formula {[Cu(bipy)(trans-H2P2O7)]2} (1b), which can be regarded as a...
Abstract Rationale Detection of atherosclerosis is essential to the management and prevention life-threatening cardiovascular events. Although non-invasive imaging modalities, such as 18 F-sodium fluoride ( F-NaF), F-fluorodeoxyglucose F-FDG) PET, CT, visualize distinct hallmarks atherosclerosis, there has yet be a singular multi-cohort interrogation their strengths limitations. Thus, we focused on identifying optimal approach for visualizing at different stages disease progression. Methods...
The L-type amino acid transporter-1 (LAT1, SLC7A5) is upregulated in a wide range of human cancers, positively correlated with the biological aggressiveness tumors, and promising target for both imaging therapy. Radiolabeled acids such as O-(2-[18F]fluoroethyl)-L-tyrosine (FET) that are transport substrates system L transporters including LAT1 have met limited success oncologic outside brain, thus new strategies needed systemic cancers. Here, we describe development evaluation novel...
Epithelial membrane protein-2 (EMP2) is upregulated in a number of tumors and therefore remains promising target for mAb-based therapy. In the current study, image-guided therapy an anti-EMP2 mAb was evaluated by PET both syngeneic immunodeficient cancer models expressing different levels EMP2 to enable better understanding its tumor uptake off accumulation clearance. The therapeutic efficacy initially high- low-expressing tumors, reduced load high EMP2-expressing 4T1 HEC-1-A tumors. To...
The reaction in water of M(II) [M = Ni or Mn] with 1,10-phenanthroline (phen) and sodium pyrophosphate (Na4P2O7) a 2:4:1 stoichiometry resulted the crystallization dinuclear complexes featuring heretofore rare bridging pyrophosphate. Single-crystal X-ray diffraction studies revealed to be {[(phen)2Ni]2(micro-P2O7)} . 27H2O (1) {[(phen)2Mn]2(micro-P2O7)} 13H2O (2) where asymmetric M(phen)2 units are bridged by bis-bidentate pyrophosphate, each metal ion exhibiting distorted octahedral...
Abstract Nutrient demand is a fundamental characteristic of rapidly proliferating cells. Vitamin B 12 vital for cell proliferation; thus neoplastic cells have an increased this essential nutrient. In study we exploited the vitamin uptake pathway to probe nutritional with radiolabeled derivative in various preclinical tumor models. We describe synthesis and biological evaluations copper‐64‐labeled –ethylenediamine–benzyl‐1,4,7‐triazacyclononane‐ N , ′, ′′‐triacetic acid (B ‐en‐Bn‐NOTA‐ 64...
Abstract The reaction in water of Co II sulfate heptahydrate with 1,10‐phenanthroline (phen) and sodium pyrophosphate (Na 4 P 2 O 7 ) a 2:4:1 stoichiometric ratio resulted the crystallization neutral dinuclear complex, {[Co(phen) ] (μ‐P )} · 6MeOH ( 1 ), as revealed by single‐crystal X‐ray diffraction study. bridging pyrophosphato ligand between two [Co(phen) 2+ units bis(bidentate) coordination mode places adjacent metal centers at 4.857 Å distance, its conformation gives rise to...
CD47 functions as a marker of "self" by inhibiting phagocytosis autologous cells. has been shown to be overexpressed various tumor types means escaping the antitumor immune response. The goal this research was investigate utility imaging using positron emission tomography (PET) in both human xenograft and murine allograft models. Anti-CD47 antibodies were conjugated with p-isothiocyanatobenzyldesferrioxamine (Df-Bz-NCS) labeled 89Zr. We employed small-animal models cancer biodistribution PET...
Recently, there has been an emergence of significant interest in silver-based antimicrobials. Our goal was to develop a radioactive tracer for investigating the biological fate such compounds. Purified 111Ag incorporated into methylated caffeine analogue, IC1 yield silver carbene complex designated as [111Ag]SCC1 and investigated biodistribution studies.
Drug discovery research on new pain targets with human genetic validation, including the voltage-gated sodium channel NaV1.7, is being pursued to address unmet medical need respect chronic and rising opioid epidemic. As part of early efforts this front, we have previously developed NaV1.7 inhibitory peptide-antibody conjugates tarantula venom-derived GpTx-1 toxin peptides an extended half-life (80 h) in rodents but only moderate vitro activity (hNaV1.7 IC50 = 250 nM) without vivo activity....
Abstract NiCl 2 · 6H O and 2,2′‐bipyridine (bipy) (1:1) were allowed to react under ambient conditions in dimethyl sulfoxide the presence of acetylacetone (Hacac). The resulting green solution was concentrated vacuo, a solid isolated by precipitation with cold acetone. X‐ray quality crystals [(bipy) Ni (μ‐Cl) (Cl) (H O) ] ( 1 ) grown subsequent vapor/liquid diffusion acetonitrile into methanolic for one week. structure consists dimeric nickel system each metal atom an octahedral geometry....
Abstract Copper(II) hydroxide reacts with 2,2′‐bipyridine (bipy) and sodium pyrophosphate in a 2:2:1 stoichiometric ratio under ambient conditions at pH 1.6 water. The resulting neutral dinuclear Cu II complex features bridging set containing monoprotonated monoatomic chloro bridge (making this the first bridged coordination an alternate, additional halide between metal centers). Single‐crystal X‐ray diffraction studies revealed to be {[Cu(bipy)] 2 (μ‐HP O 7 )(μ‐Cl)} · H O. structure...
Imaging agents based on peptide probes have desirable pharmacokinetic properties provided that they high affinities for their target in vivo. An approach to improve a ligand's affinity its is make this interaction covalent and irreversible. For purpose, we evaluated <sup>64</sup>Cu-labeled tag, <sup>64</sup>Cu-L19K-(5-fluoro-2,4-dinitrobenzene) (<sup>64</sup>Cu-L19K-FDNB), which binds covalently irreversibly vascular endothelial growth factor (VEGF) as PET imaging agent. We compared the vivo...
Meeting abstracts Talimogene laherparepvec (T-VEC) is an injectable modified oncolytic herpes simplex virus type-1 (HSV-1) hypothesized to be efficacious by at least two complimentary mechanisms of action: a) direct oncolysis the injected tumor and b) elicitation a systemic anti-tumor immune
The identification of nonopioid alternatives to treat chronic pain has received a great deal interest in recent years. Recently, the engineering series Nav1.7 inhibitory peptide-antibody conjugates been reported, and herein, preclinical efforts identify novel approaches characterize pharmacokinetic properties peptide are described. A cryopreserved plated mouse hepatocyte assay was designed measure depletion from media, with correlation being observed between percentage remaining media vivo...
Abstract The sensitivity and quantitative properties of Positron Emission Tomography (PET) imaging combined with the selectivity high affinity radiolabeled monoclonal antibodies (mAbs) make a powerful tool in molecular diseases including breast cancer. Radiolabeling mAbs positron-emitting 89Zr has recently been useful clinical trials due to its relatively long half-life (t1/2 = 3.3 days), which matches pharmacokinetic mAbs. Pertuzumab (Perjeta™) is an FDA-approved mAb for treatment human...