A5029489640- Boron Compounds in Chemistry
- Radiopharmaceutical Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Cytomegalovirus and herpesvirus research
- Organoboron and organosilicon chemistry
- Boron and Carbon Nanomaterials Research
- Crystallization and Solubility Studies
- Organophosphorus compounds synthesis
- X-ray Diffraction in Crystallography
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Characterization
- Orthopedic Infections and Treatments
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Radioactive element chemistry and processing
- Parvovirus B19 Infection Studies
- Biochemical and Molecular Research
- Herpesvirus Infections and Treatments
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Synthesis and Characterization of Heterocyclic Compounds
- Immune Response and Inflammation
- RNA modifications and cancer
Institute for Medical Biology
2016-2025
Polish Academy of Sciences
2016-2025
Institute of Medical Biology
2014-2025
University of Łódź
2014-2019
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2015
Gakushuin University
2014
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2008
Charles University
2008
Czech Academy of Sciences, Institute of Biophysics
2008
Czech Academy of Sciences, Institute of Inorganic Chemistry
2007
Boron clusters are polyhedral boron hydrides with unique properties, and they becoming increasingly widely used in biology medicine, including for neutron capture therapy (BNCT) of cancers the design novel bioactive molecules potential drugs. Among cluster types, icosahedral boranes, carboranes, metallacarboranes particularly interesting, there is a need basic studies on their interaction biologically important molecules, such as proteins. Herein, we report selected derivatives serum...
Carboranes (dicarba- closo -dodecaboranes) are a class of carbon-containing polyhedral boron-cluster compounds showing remarkable hydrophobic character, chemical and thermal stability, resistance to catabolism in biological environment. These features allow application boron clusters as new core structure biologically active molecules that interact hydrophobically with proteins, thus facilitating drug design. A review 45 references.
We report a series of 2′-deoxyribonucleoside triphosphates bearing dicarba-nido-undecaborate ([C2B9H11]1–), [3,3′-iron-bis(1,2-dicarbollide)]− (FESAN, [Fe(C2B9H11)2]2–) or [3,3′-cobalt-bis(1,2-dicarbollide)]− (COSAN, [Co(C2B9H11)2]2–) groups prepared either through the Sonogashira cross-coupling CuAAC click reaction. The modified dNXTPs were substrates for KOD XL DNA polymerase in enzymatic synthesis primer extension (PEX). nido-carborane- and FESAN-modified nucleotides gave analytically...
A general approach to the synthesis of nucleoside conjugates containing carborane and metallocarborane complexes, based on Huisgen 1,3-dipolar cycloaddition ("chemical ligation"), is described. Boron-cluster-donors bearing terminal azide or ethynyl groups were prepared in ring-opening reaction dioxane-boron-cluster adducts an anion suitable alkynol-derived alcoholate nucleophile. Analogous derivatives sulfhydryl also prepared. Nucleosides with various spacers groups, located within...
A general approach to the synthesis of nucleoside conjugates between derivatives thymidine (T), 2'-O-deoxycytidine (dC), 2'-O-deoxyadenosine (dA), and 2'-O-deoxyguanosine (dG), metallacarborane complexes is described. Metallacarborane-nucleoside are prepared by reaction dioxane-metallacarborane adduct with a base-activated 3',5'-protected nucleoside. In case T dG mixture regioisomers, which easily separable chromatographic methods, obtained, thus yielding series modifications containing...
Block condensation of MePOCI2 or MeP(NEt2)2 with appropriately protected tetra(thymidine methanephosphonates) predetermined sense chirality at asymmetric phosphonate centres gave two pairs diastereomeric mixtures, namely (SpSpSpSpSpSpSp+SpSpSpRpSpSpSp) 5a and (RpRpRpRpRpRpRp+RpRpRpSpRpRpRp) 5b.A comparison the CD spectra 5b those octathymidylic acid (7) a random mixture diastereomers octa(thymidine methanephosphonate) (6), also Tm complexes formed between 5a, 5b, 6 7, penta-...
Toll-like receptor 9 (TLR9) recognizes non-methylated viral CpG-containing DNA and serves as a pattern recognition that signals the presence of human cytomegalovirus (HCMV). Here, we present genotype distribution single-nucleotide polymorphisms (SNPs) TLR9 gene in infants relationship between HCMV infection. Four (-1237T/C, rs5743836; -1486T/C, rs187084; 1174G/A, rs352139; 2848C/T, rs352140) were genotyped 72 with symptomatic infection 70 healthy individuals. SNP genotyping was performed by...
The antiviral activity of nonfunctionalized gold nanoparticles (AuNPs) against herpes simplex virus type-1 (HSV-1) in vitro was revealed this study. We found that AuNPs are capable reducing the cytopathic effect (CPE) HSV-1 Vero cells a dose- and time-dependent manner when used pretreatment mode. demonstrated within nontoxic concentration range AuNPs. Interestingly, we noted with smaller sizes reduced CPE more effectively than larger ones. observed phenomenon can be tentatively explained by...
Nucleoside–metallacarborane conjugates (for example, TBEMC; see picture with low toxicity were synthesized and coupled as a redox label to DNA oligonucleotide. The labeled oligonucleotide acted primer for Taq polymerase.
Novel types of adenosine and 2'-deoxyadenosine derivatives containing boron clusters at positions C2', N6, or C8 were synthesized. The effect these modified compounds on platelet function was studied. Modification the C2' position with a para-carborane cluster (C(2)B(10)H(11)) results in efficient inhibition function, including aggregation, protein secretion, P-selectin expression induced by thrombin ADP. These preliminary findings new chemistry proposed form basis for development class...
Intracellular Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA (dsRNA) and activates antiviral immune responses through the production of type I interferons (IFNs) inflammatory cytokines. This binds to dsRNA molecules produced during human cytomegalovirus (HCMV) replication. TLR7 senses single-stranded (ssRNA) in endosomes, it can interact with endogenous RNAs. We determined genotype distribution single-nucleotide polymorphisms (SNPs) within TLR3 genes children HCMV infection...
The association among specific single-nucleotide polymorphisms (SNPs) in TLR2 (Arg677Trp, Arg753Gln) and TLR4 (Asp299Gln) human cytomegalovirus (CMV) infection was studied infants adults.The were genotyped 151 patients with CMV infections 78 unrelated healthy individuals. Genotyping performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments. viral load measured quantitative real-time PCR.Almost all the wild-type homozygotes without polymorphisms. No...
Selective agonism of the estrogen receptor (ER) subtypes, ERα and ERβ, has historically been difficult to achieve due high degree ligand-binding domain structural similarity. Multiple efforts have focused on use classical organic scaffolds model 17β-estradiol geometry in design ERβ selective agonists, with several proceeding various stages clinical development. Carborane offer many unique advantages including potential for novel ligand/receptor interactions but remain relatively unexplored....
Herein, we describe a new type of building block for nanoconstruction based on the composite boron cluster metal‐complex (metallacarborane) and therapeutic nucleic acid their assembly into functional nanoparticles with EGFR oncogene silencing activity. In this direction, method oligofunctionalization metallacarborane, 3,3'‐cobalt‐bis(1,2‐dicarbollide)(‐1) ion, an automated synthesis DNA‐oligonucleotides attached to metallacarborane core is developed. The obtained blocks forms...
Long-acting insulins are part of the WHO’s List Essential Medicines, which highlights their importance in treatment diabetes. Often mode action relies on albumin binding propensity they owe to chemical conjugations with fatty acids. In this work, we propose an alternative development strategy for albumin-binding through metallacarborane conjugation. study, insulin was modified newly synthesised aldehyde-bearing metallacarboranes reductive aminations yield insulin-metallacarborane conjugates...
Abstract The carboranyl cluster is a new and versatile modifying entity for nucleotides nucleic acids. Three types of (−C 2 B 10 H 11 ) group‐containing DNA‐oligonucleotides are described: (1) CBMP‐oligonucleotides, consisting the carborane cage within an internucleotide linkage; (2) CDU‐oligonucleotides, containing attached to nucleobase; (3) 2′‐CBM‐oligonucleotides, with linked sugar residue at 2′ position. method synthesis physicochemical biochemical features these novel modifications...
A productive approach for the incorporation of metal centers into nucleic acids providing new materials must be versatile, modular and synthetically simple. Metal complex-containing DNA-oligomers are predominantly constructed via three major pathways: a) synthesis a chelator-containing oligonucleotide followed by complexation, b) an end-functionalized to which complex can conjugated, c) nucleoside/metal conjugates bearing synthon DNA using automated on solid support. Representative examples...
The synthesis of mononucleoside and dinucleoside phosphates H-phosphonates bearing boron cluster complexes cobalt or iron is described. proposed method provides key synthons for the preparation bioinorganic DNA/metallacarborane conjugates a variety applications.