A5001992157- Developmental Biology and Gene Regulation
- Cancer Cells and Metastasis
- Congenital heart defects research
- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Lipid metabolism and biosynthesis
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Wnt/β-catenin signaling in development and cancer
- Pregnancy and preeclampsia studies
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Magnetic properties of thin films
- Digestive system and related health
- Pluripotent Stem Cells Research
- Nuclear Structure and Function
- Animal Genetics and Reproduction
- Glycosylation and Glycoproteins Research
- Muscle Physiology and Disorders
- Cancer, Lipids, and Metabolism
- Renal and related cancers
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Foot and Ankle Surgery
- Metabolism and Genetic Disorders
The University of Tokyo
2016-2025
Shizuoka Cancer Center
2021-2025
Nagoya University
2023-2024
Keio University
2005-2023
Laboratoire de Biochimie
2022
Tohoku University
2010-2021
Japan Tobacco (Japan)
2015-2020
Kyoto University
2016-2020
Kyoto Katsura Hospital
2020
Spintronics Research Network of Japan
2019
3D (three-dimensional) cultures are considered to be an effective method for toxicological studies; however, little evidence has been reported whether have impact on hepatocellular physiology regarding lipid or glucose metabolism. In the present study, we conducted physiological characterization of hepatoma cell lines HepG2 and HepaRG cells cultured in conditions using a hanging drop verify effect culture environment cellular responses. Apo (Apolipoprotein)B as well albumin secretion was...
Gut epithelial organoids are routinely used to investigate intestinal biology; however, current culture methods not amenable genetic manipulation, and it is difficult generate sufficient numbers for high-throughput studies. Here, we present an improved system of human induced pluripotent stem cell (iPSC)-derived involving four methodological advances. (1) We adopted a lentiviral vector readily establish optimize conditioned medium organoid culture. (2) obtained from iPSCs more efficiently by...
Abstract Perilipin2 (Plin2), also known as adipose differentiation-related protein (ADRP), or adipophilin, is a member of the PAT family involved in lipid droplet (LD) formation liver and peripheral tissues. Although Plin2 was originally identified highly expressed gene adipocytes, its physiological role mature adipocytes largely unknown. In this report, we investigated regulation expression function differentiated mouse embryonic fibroblasts (MEFs). mRNA levels increased during adipocyte...
Abstract Extracellular vesicles (EVs) contain various regulatory molecules and mediate intercellular communications. Although EVs are secreted from cell types, including skeletal muscle cells, present in the blood, their identity is poorly characterized vivo, limiting identification of origin blood. Since largest organ body, it could substantially contribute to circulating as source. However, due lack defined markers that distinguish muscle-derived (SkM-EVs) others, whether releases vivo how...
Paneth cells are intestinal epithelial that release antimicrobial peptides, such as α-defensin part of host defense. Together with mesenchymal cells, provide niche factors for stem cell homeostasis. Here, we report two subtypes murine differentiated by their production and utilization fucosyltransferase 2 (Fut2), which regulates α(1,2)fucosylation to create cohabitation niches commensal bacteria prevent invasion the intestine pathogenic bacteria. The majority Fut2- were localized in...
Mesp2 is a transcription factor that plays fundamental roles in somitogenesis, and its expression strictly restricted to the anterior presomitic mesoderm just before segment border formation. The transcriptional on–off cycle linked segmentation clock. In our current study, we show T-box factor, Tbx6, essential for expression. Tbx6 directly binds gene upstream region mediates Notch signaling, subsequent transcription, mesoderm. Our data therefore reveal mechanism, via Tbx6-dependent acts on...
Serotonin is synthesized by two distinct tryptophan hydroxylases, one in the brain and periphery. The latter known to be unable cross blood-brain barrier. These serotonin systems have apparently independent functions, although functions of peripheral yet fully elucidated. In this study, we investigated physiological effect on concentrations metabolites circulation liver. After fasting, mice were ip injected with 1 mg serotonin. plasma glucose concentration was significantly elevated between...
Intestinal organoids are physiologically relevant tools used for cellular models. However, the suitability of to examine biological functions over existing established cell lines lacks sufficient evidence. Cytochrome P450 3A4 (CYP3A4) induction by pregnane X receptor ligands, glucose uptake via sodium/glucose cotransporter 1, and microsomal triglyceride transfer protein-dependent ApoB-48 secretion, which critical human intestinal metabolism, were observed in organoid-derived two-dimensional...
Abstract Organoids are regarded as physiologically relevant cell models and useful for compound screening drug development; however, their applications currently limited because of the high cost culture. We previously succeeded in reducing human intestinal organoid culture using conditioned medium (CM) L cells co-expressing Wnt3a, R-spondin1, Noggin. Here, we further reduced by replacing recombinant hepatocyte growth factor with CM. Moreover, showed that embedding organoids collagen gel, a...
Notch signaling exerts multiple roles during different steps of mouse somitogenesis. We have previously shown that segmental boundaries are formed at the interface activity boundary, suggesting importance on/off state for boundary formation. However, a recent study has embryos expressing Notch-intracellular domain (NICD) throughout presomitic mesoderm (PSM) can still form more than ten somites, indicating NICD is dispensable To clarify this discrepancy in our current study, we created...
Sterol regulatory element-binding protein-1 (SREBP-1) has been thought to be a critical factor that assists adipogenesis. During adipogenesis SREBP-1 stimulates lipogenic gene expression, and peroxisome proliferator-activated receptor γ (PPARγ) enhances perilipin (plin) resulting in generating lipid droplets (LDs) store triacylglycerol (TAG) adipocytes. Plin coats adipocyte LDs protects them from lipolysis. Here we show white adipose tissue (WAT) of plin−/− mice nuclear active its target but...
Visceral fat accumulation as observed in Crohn's disease and obesity is linked to chronic gut inflammation, suggesting that of adipocytes can trigger local inflammatory signaling. However, direct interactions between intestinal epithelial cells (IECs) have not been investigated, part because IEC physiology difficult replicate culture. In this study, we originally prepared intact, polarized, cytokine responsive monolayers from primary or induced pluripotent stem cell-derived organoids by...
Elaborate metamerism in vertebrate somitogenesis is based on segmental gene expression the anterior presomitic mesoderm (PSM). Notch signal pathways with ligands Dll1 and Dll3, transcription factor Mesp2 are implicated rostrocaudal patterning of somite. We have previously shown that changes domain from a presumptive one somite into rostral half results differential activation two types pathways, dependent or independent presenilin 1 (Psen1), which mediator. To further refine our hypothesis,...
Immunoregulatory probiotics (immunobiotics) have been proposed to improve piglets' immune system avoid intestinal infections and reduce unproductive inflammation after weaning. Previously, it was demonstrated that Lactobacillus jensenii TL2937 (LjTL2937) attenuated the inflammatory response triggered by activation of Toll-like receptor 4 (TLR-4) in porcine epithelial (PIE) cells antigen presenting (APCs) from Peyer's patches (PP). In view critical importance PIE-APCs interactions regulation...
The nuclear receptor-type transcription factor retinoic acid receptor-related orphan receptor alpha (RORalpha) is a multifunctional molecule involved in tissue development and cellular function, such as inflammation, metabolism, differentiation; however, the role of RORalpha during adipocyte differentiation has not yet been fully understood. Here we show that inhibits transcriptional activity CCAAT/enhancer-binding protein beta (C/EBPbeta) without affecting its expression, thereby blocking...
In mice, peripheral 5-HT induces an increase in the plasma concentrations of glucose, insulin and bile acids, a decrease triglyceride, NEFA cholesterol concentrations. However, given unique characteristics metabolism ruminants relative to monogastric animals, physiological role on glucose lipid sheep remains be established. Therefore, this study, we investigated effect circulating metabolites using five receptor (5HTR) antagonists sheep. After fasting for 24 h, were intravenously injected...
Given the high prevalence of esophageal adenocarcinoma and Barrett's esophagus (BE), a precancerous lesion, among males, it is important to understand characteristics BE by sex develop an effective endoscopic surveillance program in Japan. The present study examined age distribution risk factors for Japanese health check-up cohort sex. data set at baseline our preceding multicenter study, comprising total 33,478 individuals who underwent upper screening 17 institutes across Japan, was...