A5025303456- Sperm and Testicular Function
- Hormonal and reproductive studies
- Regulation of Appetite and Obesity
- Hypothalamic control of reproductive hormones
- Reproductive Physiology in Livestock
- Sexual Differentiation and Disorders
- Reproductive biology and impacts on aquatic species
- Genetic and phenotypic traits in livestock
- Biochemical Analysis and Sensing Techniques
- Pancreatic function and diabetes
- Effects of Environmental Stressors on Livestock
- Adipokines, Inflammation, and Metabolic Diseases
- Testicular diseases and treatments
- Animal Behavior and Reproduction
- Dietary Effects on Health
- Ovarian function and disorders
- Amphibian and Reptile Biology
- Neuroendocrine regulation and behavior
- Neonatal Health and Biochemistry
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Genomics and Rare Diseases
- Diet and metabolism studies
- RNA Interference and Gene Delivery
- Reproductive Biology and Fertility
- Genomic variations and chromosomal abnormalities
Forman Christian College
2012-2025
Bahauddin Zakariya University
2024
National University of Sciences and Technology
2024
Sri Venkateswara Institute of Medical Sciences
2022-2024
University of Veterinary and Animal Sciences
2021-2023
Pakistan Institute of Medical Sciences
2021-2023
University of Lahore
2013-2023
Isra University
2018-2022
Istanbul University-Cerrahpaşa
2020
Institute of Molecular Biology and Biotechnology
2019
Gonadal quiescence prior to puberty in primates results from a diminished secretion of the pituitary gonadotropic hormones, follicle-stimulating hormone and luteinizing hormone, which, turn, is occasioned by an interruption pulsatile release gonadotropin-releasing (GnRH) hypothalamus during this phase development. A discharge GnRH may be provoked prepubertal monkeys, however, i.v. injection N-methyl-D-aspartate (NMDA), analog putative excitatory neurotransmitter, aspartate. Since action NMDA...
Single gene mutations leading to severe obesity have so far been identified in 3-5% cases European populations. However, prevalence of these pathogenic has not systematically examined specific consanguineous Here we describe the incidence obesity-associated through a step-wise sequence analysis, cohort 73 Pakistani children with from families.Initially, all subjects were screened for coding regions leptin (LEP) and melanocortin 4 receptor (MC4R) genes by direct sequencing. Subjects negative...
OBJECTIVE Accurate etiological diagnosis of monogenic forms diabetes and obesity is useful as it can lead to marked improvements in patient care genetic counseling. Currently, molecular based on Sanger sequencing restricted only a few genes, this technology expensive, time-consuming, labor-intensive. High-throughput next-generation (NGS) provides an opportunity develop innovative cost-efficient methods for sensitive multigene screening. RESEARCH DESIGN AND METHODS We assessed new method PCR...
The long-term clinical outcomes of severe obesity due to leptin signaling deficiency are unknown. We carry out a retrospective cross-sectional investigation large cohort children with (LEP), LEP receptor (LEPR), or melanocortin 4 (MC4R) (n = 145) evaluate the progression disease. affected individuals undergo physical, clinical, and metabolic evaluations. report very high mortality in (26%) LEPR (9%), mainly pulmonary gastrointestinal infections. In addition, 40% surviving experience...
To determine whether neuroexcitatory amino acids may play a role in generating intermittent hypothalamic GnRH release, the effect of N-methyl-D-aspartate (NMDA) receptor blockade on pulsatile LH secretion was examined male rats. The ability NMDA antagonist, DL-2-amino-5-phosphonopentanoic acid (AP5), to inhibit activation hypothalamic-pituitary gonadotroph axis that follows peripheral administration NMDA, first established intact Subsequently, acutely castrated rats (n = 12) bearing venous...
Objective Mutations in leptin receptor gene ( LEPR ) result early onset extreme adiposity. However, their prevalence different populations is not known. Indeed, screening by gold standard Sanger sequencing has been limited its large size and the cost. One‐step PCR‐based targeted enrichment could be an option for rapid cost effective molecular diagnosis of monogenic forms obesity. Methods The study based on 39 unrelated severely obese Pakistani children, previously shown to negative LEP...
ABSTRACT The present study was designed to investigate the relative contributions of FSH and testosterone in initiation testicular growth function primates. Four groups ( n = 4/group) juvenile rhesus monkeys Macaca mulatta ), 12–18 months old, were treated with vehicle, a highly purified human preparation (hFSH; Fertinorm, 3 IU/kg per day), (testosterone enanthate, 125 mg/week) or plus testosterone, for period 12 weeks. Compared vehicle treatment, administration hormones significantly P...
Study of monogenic forms obesity has demonstrated the pivotal role central leptin-melanocortin pathway in controlling energy balance, appetite and body weight 1 .The majority loss-of-function mutations (mostly recessive or co-dominant) have been identified genes that are directly involved signaling.These genes, however, only explain < 5% cases, predominantly from outbred populations 2 .We previously showed that, a consanguineous population Pakistan, known obesity-related ~30% cases with...
Monogenic forms of obesity have been identified in ≤10% severely obese European patients. However, the overall spectrum deleterious variants (point mutations and structural variants) responsible for childhood severe remains elusive. In this study, we genetically screened 225 children from consanguineous Pakistani families through a combination techniques, including an in-house-developed augmented whole-exome sequencing method (CoDE-seq) that enables simultaneous detection copy number...
Abstract Disruption of brain-expressed G protein-coupled receptor-10 (GPR10) causes obesity in animals. Here, we identify multiple rare variants GPR10 people with severe and normal weight controls. These impair ligand binding protein-dependent signalling cells. Transgenic mice harbouring a loss function variant found an individual obesity, gain excessive due to decreased energy expenditure rather than increased food intake. This evidence supports role for human homeostasis. Therapeutic...
Abstract This study analyzed the hormonal requirements necessary for development of primate testicular peritubular cells. Alpha‐smooth muscle actin, as a specific differentiation marker cells, was immunohistochemically detected in testes immature rhesus and adult cynomolgus monkeys. Positive staining localized wall blood vessels myoid cells animals. In testis vehicle‐treated monkeys no positive could be Following follicle‐stimulating hormone (FSH) or testosterone treatment, immunostaining...
Four groups(N = 3 per group) of juvenile rhesus monkeys (Macaca mulatta, 14-20 months old) received either vehicle or highly purified human follicle-stimulating hormone (FSH; 10 IU kg-1 day-1), chorionic gonadotropin (hCG; 250 every alternate day) both hormones for a period 4 weeks. Testicular volume and weight increased more than twofold after single sixfold combined treatment. Serum intratesticular testosterone were at supraphysiological levels in hCG-treated animals rose even treatment;...
Recent advances in genetic analysis have significantly helped progressively attenuating the heritability gap of obesity and brought into focus monogenic variants that disrupt melanocortin signaling. In a previous study, next-generation sequencing revealed etiology ∼50% children with severe from consanguineous population Pakistan. Here we assess rare obesity-causing genes young adults same region. Genomic DNA 126 randomly selected adult obese subjects (BMI 37.2 ± 0.3 kg/m2; age 18.4 years)...
Objective: To assess the effects of nutritional status on surgical patients using a Subjective Global Assessment (SGA) scale Methods: This cross-sectional study was conducted in Department General Surgery, PIMS Islamabad from March 2022 to February 2024. All admitted ward were recruited by convenient sampling during period. demographic data and clinical histories recorded including SGA category, gender, age, site infection, length hospital stay death. SPSS version 23 used for statistical...