A5072239287- Microbial Metabolic Engineering and Bioproduction
- Cystic Fibrosis Research Advances
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Plant biochemistry and biosynthesis
- Nanoparticles: synthesis and applications
- Biochemical and biochemical processes
- Marine animal studies overview
- Plant Pathogenic Bacteria Studies
- Fungal and yeast genetics research
- Environmental DNA in Biodiversity Studies
- Plant-Microbe Interactions and Immunity
- Vibrio bacteria research studies
- Advanced Proteomics Techniques and Applications
- Graphene and Nanomaterials Applications
- Mycorrhizal Fungi and Plant Interactions
- Microbial bioremediation and biosurfactants
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Oral microbiology and periodontitis research
- Advanced Nanomaterials in Catalysis
University of Minho
2011-2023
University of Lisbon
2003-2021
Instituto de Engenharia de Sistemas e Computadores Investigação e Desenvolvimento
2021
Universidade de São Paulo
2019-2020
Clinical Academic Center of Braga
2019
Instituto Superior Técnico
2003-2011
Institute for Biotechnology and Bioengineering
2003-2011
Instituto Politécnico de Lisboa
2003
Sapienza University of Rome
2002
Marconi University
2002
Abstract To gain insight into the global mechanism underlying phenol toxicity and tolerance in bacteria, we have generated a two‐dimensional protein reference map used it to identify variations expression levels Pseudomonas putida KT2440 following exposure sub‐lethal inhibitory concentrations of this solvent. Inspection gel electrophoresis gels revealed that 1 h sudden cell two different phenol, leading inhibition exponential growth (600 mg/L) or arrest for, at least, 4 before inhibited...
The International Pseudomonas aeruginosa Consortium is sequencing over 1000 genomes and building an analysis pipeline for the study of genome evolution, antibiotic resistance virulence genes. Metadata, including genomic phenotypic data each isolate collection, are available through Database (http://ipcd.ibis.ulaval.ca/). Here, we present our strategy results that emerged from first 389 genomes. With as yet unmatched resolution, confirm P. strains can be divided into three major groups...
Burkholderia multivorans is an opportunistic pathogen capable of causing severe disease in patients with cystic fibrosis (CF). Patients may be chronically infected for years, during which the bacterial population evolves response to unknown forces. Here we analyze genomic and functional evolution a B. infection that was sequentially sampled from CF patient over 20 years. The diversified into at least four primary, coexisting clades distinct evolutionary dynamics. average substitution rate...
The adaptive responses of the bacterium Acinetobacter calcoaceticus to different aliphatic alcohols on level membrane fatty acids were studied in detail. toxicity increased with an increasing hydrophobicity. As are known increase fluidity they consequently should cause same effect level. Yet, cells A. react completely alcohols: presence long-chained their degree saturation, while short-chained decrease saturation. So, there no observable differences bacteria so-called anaerobic pathway, like...
Abstract Toxicogenomics has the potential to elucidate gene–environment interactions identify genes that are affected by a particular chemical at early stages of toxicological response and establish parallelisms between different organisms. The fungicide mancozeb, widely used in agriculture, is an ethylene‐bis‐dithiocarbamate complex with manganese zinc. Exposure this pesticide been linked development idiopathic Parkinson's disease cancer. Given many signalling pathways their molecular...
Hydrogel dressings are crosslinked hydrophilic polymers able of swelling in presence water and can be used many different types wound care. In turn, care, silver has been for a long time as an effective antimicrobial agent. Recent studies have demonstrated increase its action when it is at nanometer scale, that is, nanoparticles (AgNPs), which anti-inflammatory effect on infected wounds, rashes, mesh skin grafts. The objective this work was to study hydrogel containing AgNPs from nitrate,...
Broad-host-range plasmid RK2-based promoter probe vectors with a known nucleotide sequence were constructed. In the absence of an upstream promoter, expression two tested reporter genes (luc and lacZ) in Escherichia coli was virtually zero, while insertion Ptrc resulted strong inducer-dependent expression. The lacZ-based mobilized into Pseudomonas fluorescens ST, putida KT2442, Sphingomonas spp. Burkholderia LB400, analyses indicated that properties observed E. are maintained across species...
Ag<sup>+</sup>had stronger effects than AgNPs on the growth of<italic>Pseudomonas</italic>sp. M1. Responses of metabolic, energetic and stress pathways to Ag<sup>+</sup>and AgNP exposure suggested distinct mechanisms toxicity.
Quantitative proteomics was used to gain insights into the global adaptive response phenol in phenol-biodegrading strain Pseudomonas sp. M1 when an alternative carbon source (pyruvate or succinate) is present. A phylogenetic analysis indicated citronellolis as closest species environmental M1, while P. aeruginosa with genome sequence available. After two-dimensional gel electrophoresis (2-DE) separation, protein identification by MS/MS ion search allowed assignment of 87 out 136 selected...
Sinorhizobium meliloti is capable of establishing a symbiotic nitrogen fixation relationship with Medicago sativa. During this process, it must cope diverse environments and has evolved different types transport systems that help its propagation in the plant roots. TolC protein family members are outer-membrane components several involved export molecules, playing an important role bacterial survival. In work, we have characterized from S. 2011. An insertional mutation tolC gene strongly...
The drilling, processing and transportation of oil are the main sources pollution in water soil. current work analyzes microbial diversity aromatic compounds degradation potential metagenomes communities wastewater treatment plant (WWTP) a crude refinery. By focusing on phenol, we observed involvement diverse indigenous at different steps WWTP. anaerobic bacterial archaeal genera were replaced by aerobic facultative bacteria through biological processes. phyla Proteobacteria, Bacteroidetes...
ABSTRACT The effects of different carbon sources on expression the styrene catabolism genes in Pseudomonas fluorescens ST were analyzed by using a promoter probe vector, pPR9TT, which contains transcription terminators upstream and downstream β-galactosidase reporter system. Expression stySR operon, codes for two-component regulatory system, was found to be constitutive not subject catabolite repression. This confirmed results an analysis transcript P. cells grown sources. operon upper...
The intensive use of herbicides may give rise to a number toxicological problems in non-target organisms and has led the emergence resistant weeds. To gain insights into mechanisms adaptation herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), we have identified variations protein expression level eukaryotic experimental model Saccharomyces cerevisiae exposed aggression, based on two-dimensional gel electrophoresis. We show results suggesting that during period preceding resumption inhibited...
Abstract Chronic respiratory infections caused by Burkholderia cenocepacia in patients with cystic fibrosis (CF) are characterized low responsiveness to antibiotic therapy and, general, a more rapid decline of lung function. To get clues into the molecular mechanisms underlying adaptive strategies employed deal stressing conditions CF including therapy, quantitative proteomics (2‐D DIGE) was used compare expression programs two clonal isolates retrieved from chronically infected patient....
Summary Fungal diversity in M editerranean forest soils is poorly documented, particularly when considering saprobic and pathogenic organisms. Next‐generation sequencing ( NGS ) methods applied to soil fungi provide the opportunity unveil most inconspicuous functional guilds (e.g. saprobes) life forms C orticiaceae) of this tremendous diversity. We used fruitbody surveys over 2 years 454 metabarcoding astanea sativa orchards evaluate respectively reproductive (fruitbodies) vegetative...
Respiratory infections with Burkholderia cepacia complex (Bcc) bacteria in cystic fibrosis (CF) are associated a worse prognosis and increased risk of death. In this work, we assessed the virulence potential three B. cenocepacia clonal isolates obtained from CF patient between onset infection (isolate IST439) before death syndrome 3.5 years later IST4113 followed by IST4134), based on their ability to invade epithelial cells compromise monolayer integrity. The two retrieved during late-stage...
Abstract The composition of mammalian microbiota has been related with the host health status. In this study, we assessed oral microbiome 3 cetacean species most commonly found stranded in Iberian Atlantic waters ( Delphinus delphis , Stenella coeruleoalba and Phocoena phocoena ), using 16S rDNA-amplicon metabarcoding. All microbiomes were dominated by Proteobacteria Firmicutes Bacteroidetes Fusobacteria bacteria, which also predominant cavity Tursiops truncatus . A Constrained Canonical...
Abstract Motivation: The ability to identify complex motifs, i.e. non-contiguous nucleotide sequences, is a key feature of modern motif finders. Addressing this problem extremely important, not only because these motifs can accurately model biological phenomena but its extraction highly dependent upon the appropriate selection numerous search parameters. Currently available combinatorial algorithms have proved be efficient in exhaustively enumerating (including motifs), which fulfill certain...