A5005067201- Bacterial biofilms and quorum sensing
- Cystic Fibrosis Research Advances
- Antibiotic Resistance in Bacteria
- Inhalation and Respiratory Drug Delivery
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Lung Cancer Treatments and Mutations
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Neonatal Respiratory Health Research
- Pediatric health and respiratory diseases
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Tracheal and airway disorders
- Transplantation: Methods and Outcomes
- Mycobacterium research and diagnosis
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
- Tuberculosis Research and Epidemiology
- Immunotherapy and Immune Responses
- RNA modifications and cancer
McGill University
2016-2025
McGill University Health Centre
2016-2025
International Union for Conservation of Nature
2023-2024
Emory University
2023
Thai Nguyen University
2023
Tay Nguyen University
2023
RMIT University
2021-2023
University of Miami
2008-2023
Sylvester Comprehensive Cancer Center
2023
MIT University
2023
Bacteria become highly tolerant to antibiotics when nutrients are limited. The inactivity of antibiotic targets caused by starvation-induced growth arrest is thought be a key mechanism producing tolerance. Here we show that the tolerance nutrient-limited and biofilm Pseudomonas aeruginosa mediated active responses starvation, rather than passive effects arrest. protective controlled starvation-signaling stringent response (SR), our experiments link SR-mediated reduced levels oxidant stress...
BACKGROUND: Preclinical studies in animal models have demonstrated tumor regression following intratumoral administration of an adenovirus vector containing wild-type p53 complementary DNA (Ad-p53). Therefore, a phase I clinical trial, we administered Ad-p53 to 28 patients with non-small-cell lung cancer (NSCLC) whose cancers had progressed on conventional treatments. METHODS: Patients received up six, monthly injections by use computed tomography-guided percutaneous fine-needle injection...
Rationale: Birt-Hogg-Dubé syndrome (BHDS) is an autosomal, dominantly inherited genodermatosis that predisposes to fibrofolliculomas, kidney neoplasms, lung cysts, and spontaneous pneumothorax.Objectives: We evaluated 198 patients from 89 families with BHDS characterize the risk factors for pneumothorax genotype–pulmonary associations.Methods: Helical computed tomography scans of chest were used screen pulmonary abnormalities. BHD mutation data associations. examined relationship categorical...
Background. Athletics-associated methicillin-resistant Staphylococcus aureus (MRSA) infections have become a high-profile national problem with substantial morbidity. Methods. To investigate an MRSA outbreak involving college football team, we conducted retrospective cohort study of all 100 players. A case was defined as cellulitis or skin abscess diagnosed during the period 6 August (the start camp) through 1 October 2003. Results. We identified 10 patients (2 whom were hospitalized). The...
To determine the safety and tolerability of adenovirus-mediated p53 (Adp53) gene transfer in sequence with cisplatin when given by intratumor injection patients non-small-cell lung cancer (NSCLC).Patients advanced NSCLC abnormal function were enrolled onto cohorts receiving escalating dose levels Adp53 (1 x 10(6) to 1 10(11) plaque-forming units [PFU]). Patients administered intravenous 80 mg/m(2) on day study vector 4 for a total up six courses (28 days per course). Apoptosis was determined...
Abstract Purpose: The DNA methylation paradox, manifested as derepression of cancer-testis antigens, and silencing tumor suppressors during malignant transformation, provides the rationale for utilization chromatin remodeling agents cancer therapy. A phase I trial was done to examine pharmacokinetics, toxicities, gene expression mediated by 5-aza-2′-deoxycytidine (DAC) in patients with thoracic malignancies. Experimental Design: Thirty-five cancers refractory standard therapy received...
An outbreak of community-associated methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infection (SSTI) occurred in a college football team from August to September 2003. Eleven case-players were identified, boils the most common sign. Linemen had highest attack rate (18%). Among 99 (93% team) players with cultured specimens, 8 (8%) positive MRSA nasal cultures. All available case-players' isolates characterized pulsed-field type USA300. A case-control study found that...
Regulatory sequences recognized by the unique pair of paralogous factors, CTCF and BORIS, have been implicated in epigenetic regulation imprinting X chromosome inactivation. Lung cancers exhibit genome-wide demethylation associated with derepression a specific class genes encoding cancer-testis (CT) antigens such as NY-ESO-1. CT are normally expressed BORIS-positive male germ cells deficient meCpG contents, but strictly silenced somatic cells. The present study was undertaken to ascertain if...
The synthesis and characterization of a novel two-photon-absorbing fluorene derivative, LT1, selective for the lysosomes HCT 116 cancer cells, is reported. Linear nonlinear photophysical photochemical properties probe were investigated to evaluate potential two-photon fluorescence microscopy (2PFM) lysosomal imaging. cytotoxicity was using this live biological imaging applications. Colocalization studies with commercial Lysotracker Red in cells demonstrated specific localization an extremely...
Pseudomonas aeruginosa, a human opportunistic pathogen, possesses number of antioxidant defense enzymes under the control multiple regulatory systems. We recently reported that inactivation P. aeruginosa stringent response (SR), starvation stress controlled by alarmone (p)ppGpp, caused impaired defenses and antibiotic tolerance. Since catalases are key in we compared levels H2O2 susceptibility catalase activity wild-type ΔrelA ΔspoT (ΔSR) mutant cells. found SR was required for optimal...
Identification of early, asymptomatic interstitial lung disease (ILD) in populations at risk developing idiopathic pulmonary fibrosis (IPF) may improve the understanding natural history IPF.To determine clinical, radiographic, physiologic, and pathologic features ILD family members patients with familial IPF.One hundred sixty-four subjects from 18 kindreds affected IPF were evaluated for ILD. Bronchoalveolar lavage fluid cells analyzed using flow cytometry. Lung biopsies performed six...
The International Pseudomonas aeruginosa Consortium is sequencing over 1000 genomes and building an analysis pipeline for the study of genome evolution, antibiotic resistance virulence genes. Metadata, including genomic phenotypic data each isolate collection, are available through Database (http://ipcd.ibis.ulaval.ca/). Here, we present our strategy results that emerged from first 389 genomes. With as yet unmatched resolution, confirm P. strains can be divided into three major groups...
Rationale: Progress has been made in understanding how the cystic fibrosis (CF) basic defect produces lung infection susceptibility. However, it remains unclear why CF exclusively leads to chronic infections that are noninvasive and highly resistant eradication. Although biofilm formation suggested as a mechanism, recent work raises questions about role of biofilms CF.Objectives: To learn airway conditions attributed transmembrane regulator dysfunction could lead infection, determine if...
Cystic fibrosis lung disease is characterized by chronic airway infections with the opportunistic pathogen Pseudomonas aeruginosa and severe neutrophilic pulmonary inflammation. P. undergoes extensive genetic adaptation to cystic (CF) environment, adaptive mutations in quorum sensing regulator gene lasR commonly arise. We sought define how alter host-pathogen relationships. demonstrate that mutants induce exaggerated host inflammatory responses respiratory epithelial cells, increased...
Significance Antibiotic tolerance causes antibiotic treatment failure and promotes the emergence of genotypic resistance in chronic infections, such as those caused by pathogen Pseudomonas aeruginosa . Laboratory stationary-phase bacteria exhibit a slow growing metabolically quiescent state associated with high levels multidrug likely analogous to vivo environment during infection. We demonstrate that superoxide dismutases confer bacteria, identify link between (p)ppGpp-mediated stress...
Nanopillars have been shown to mechanically damage bacteria, suggesting a promising strategy for future antibacterial surfaces. However, the mechanisms underlying this phenomena remain unclear, which ultimately limits translational potential toward real-world applications. Using real-time and end-point analysis techniques, we demonstrate that in contrast initial expectations, bacteria on multiple hydrophilic "mechano-bactericidal" surfaces remained viable unless exposed moving air–liquid...
Global alterations in chromatin structure profoundly influence gene expression thoracic neoplasms, silencing tumor suppressors while facilitating the of various cancer testis antigens such as NY-ESO-1. Although recent studies have shown that histone deacetylase inhibitors can potentiate suppressor induction mediated by demethylating agents cells, ability these to augment antigen not been fully defined. The authors designed current study determine whether inhibitor, depsipeptide FR901228...
The direct repeat (DR) region has been determined to be an important chromosomal domain for studying the evolution of Mycobacterium tuberculosis. Despite this, very little is known about microevolutionary events associated with clonal expansion and how such influence interpretation both restriction fragment length polymorphism (RFLP) spoligotype data. This study examined structure DR in three independently evolving lineages M. tuberculosis a combination DR-RFLP, spoligotyping, partial DNA...