A5020692401- Antifungal resistance and susceptibility
- Plant Pathogens and Fungal Diseases
- Fungal Infections and Studies
- Nail Diseases and Treatments
- Infectious Diseases and Mycology
- Bacterial Identification and Susceptibility Testing
- Malaria Research and Control
- Parasites and Host Interactions
- Mosquito-borne diseases and control
- Parasitic Diseases Research and Treatment
- Mycotoxins in Agriculture and Food
- Parasite Biology and Host Interactions
- Asthma and respiratory diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Gut microbiota and health
- Helminth infection and control
- Bee Products Chemical Analysis
- Genomics and Phylogenetic Studies
- Enterobacteriaceae and Cronobacter Research
- Dermatological diseases and infestations
- Fungal Biology and Applications
- Sinusitis and nasal conditions
- Mycorrhizal Fungi and Plant Interactions
- Indoor Air Quality and Microbial Exposure
- Allergic Rhinitis and Sensitization
Aix-Marseille Université
2016-2025
Méditerranée Infection Foundation
2015-2025
Assistance Publique Hôpitaux de Marseille
2016-2025
Service de Santé des Armées
2023-2025
Inserm
2001-2024
Institut de Recherche pour le Développement
2010-2024
Centre National de la Recherche Scientifique
2006-2024
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2010-2024
Cheikh Anta Diop University
2024
Centre de Recherche et d’Enseignement de Géosciences de l’Environnement
2023
Human and animal fungal pathogens are a growing threat worldwide leading to emerging infections creating new risks for established ones. There is need rapid accurate identification of enable early diagnosis targeted antifungal therapy. Morphological biochemical methods time-consuming require trained experts. Alternatively, molecular methods, such as DNA barcoding, powerful easy tool monophasic identification, offer practical approach species less demanding in terms taxonomical expertise....
MALDI-TOF MS recently emerged as a valuable identification tool for bacteria and yeasts revolutionized the daily clinical laboratory routine. But it has not been established routine mould identification. This study aimed to validate standardized procedure MS-based in laboratory.First, pre-extraction extraction procedures were optimized. With this procedure, 143 strains reference spectra library was built. Then, isolates cultured from sequential samples prospectively subjected...
Diagnosis of invasive fungal infection (IFI) remains a challenge. A retrospective study was performed on 279 patients at three French university hospitals to evaluate the performance (1-->3)-beta-D-glucan assay (BG assay; Fungitell; Associates Cape Cod, Inc.) for diagnosis IFI. The results one serum per subject were analyzed 117 who had probable or proven IFI according European Organization Research and Treatment Cancer criteria (70 pulmonary aspergilloses [IPA], 27 bloodstream infections,...
Spatial and temporal heterogeneities in the risk of malaria have led WHO to recommend fine-scale stratification epidemiological situation, making it possible set up actions clinical or basic researches targeting high-risk zones. Before initiating such studies is necessary define local patterns transmission infection (in time space) order facilitate selection appropriate study population intervention allocation. The aim this was identify, spatially temporally, zones malaria, at household...
In order to elucidate the distribution of Cryptococcus neoformans and C. gattii in Mediterranean basin, an extensive environmental survey was carried out during 2012-2015. A total 302 sites located 12 countries were sampled, 6436 samples from 3765 trees collected 5% found be colonized by cryptococcal yeasts. isolated 177 13. 27% Ceratonia, 10% Olea, Platanus Prunus a lower percentage other tree genera. The 13 isolates five Eucalyptus, four two Pinus Olea trees. distributed all around whereas...
The identification of filamentous fungi by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) relies mainly on a robust and extensive database reference spectra. To this end, large in-house library containing 760 strains representing 472 species was built evaluated 390 clinical isolates comparing MALDI-TOF MS with the classical method based morphological observations. use resulted in correct 95.4% at level, without considering LogScore values. Taking...
ABSTRACT Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry has emerged as a reliable technique to identify molds involved in human diseases, including dermatophytes, provided that exhaustive reference databases are available. This study assessed an online identification application based on original algorithms and extensive in-house database comprising 11,851 spectra (938 fungal species 246 genera). Validation criteria were established using initial...
This study aimed to validate the effectiveness of a standardised procedure for MALDI-TOF mass spectrometry (MS)-based identification on large sample filamentous fungi routinely identified in university hospitals' laboratories. Non-dermatophyte prospectively isolated routine activity five teaching hospitals France were first by conventional methods each laboratory and then MS one centre. DNA sequence-based resolved discrepancies between both methods. In this study, 625 analysed 58 species,...
Abstract Herein, the mycobiota was characterized in fecal samples from sick patients and healthy subjects, collected different geographical locations using both culturomics amplicon-based metagenomics approaches. Using approach, a total of 17,800 fungal colonies were isolated 14 samples, resulted isolation 41 species, which 10 species had not been previously reported human gut. Deep sequencing fungal-directed ITS1 ITS2 amplicons led to detection 142 OTUs 173 regions, respectively. Ascomycota...
Several Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry protocols, which differ in identification criteria, have been developed for mold and dermatophyte identification. Currently, the most widely used approach is Bruker technology, although no consensus concerning log(score) threshold has established. Furthermore, it remains unknown how far increasing number of spots to compare results might improve performance. In this study, we in-house reference databases as...
Polystyrene (PS) is a major commodity polymer widely used in various applications ranging from packaging to insulation thanks its low cost, high stiffness, and transparency as well relatively softening temperature. Similarly all polymers prepared by radical polymerization, PS constituted of C–C backbone thus not degradable. To confer degradability such materials, the copolymerization vinyl monomers with cyclic monomer that could undergo ring-opening an efficient method introduce purposely...
The risk of Plasmodium falciparum infection is variable over space and time this variability related to environmental variability. Environmental factors affect the biological cycle both vector parasite. Despite strong relationship, effects have rarely been included in malaria transmission models. Remote sensing data on environment were incorporated into a temporal model transmission, forecast evolution epidemiology, locality Sudanese savannah area. A dynamic cohort was constituted June 1996...
Filamentous fungi and yeasts are increasingly isolated from respiratory secretions of patients with cystic fibrosis (CF), persistent fungal colonization the airways such is thought to exacerbate lung damage. While many independent studies have identified Aspergillus fumigatus complex as principal colonizing fungus in CF, increased awareness role CF pathology coupled improved mycological culture identification methods resulted a number other being reported sputum samples, including A....
MALDI-TOF MS-based yeast identification requires a pretreatment step for which four are described in the literature, i.e., direct smear, fast formic acid and two complete acid/acetonitrile extractions. In this study we compared impact of these procedures on performance samples from colonies grown Sabouraud or chromogenic media. A total 103 isolates recovered clinical were identified parallel using procedures. The proportions both correct identifications (regardless LogScore values) reliable...
The poor reproducibility of matrix-assisted desorption/ionization time-of-flight (MALDI-TOF) spectra limits the effectiveness MALDI-TOF MS-based identification filamentous fungi with highly heterogeneous phenotypes in routine clinical laboratories. This study aimed to enhance by assessing several architectures reference spectrum libraries.We established libraries that included 30 fungus species various characterized distinct combinations following: i) technical replicates, i.e., number...
The conventional identification of dermatophytes requires a long turnaround time and highly skilled mycologists. We have recently developed tandardized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) assay to routinely identify molds potential clinical significance. This study objective was determine if this same could also be employed in the routine laboratory setting. effects inclusion cycloheximide culture medium incubation were tested after...
Microbiome and the immune system are constantly shaping each other, in a mutual aim to thrive, defining unstable equilibrium of healthy individual. is growingly involved dysimmune conditions such as allergy, asthma, autoimmunity, primary or acquired deficiencies. The current epidemics allergic diseases asthma has long been linked microbial environment through hygiene hypothesis. Progress understanding microbiome-immune crosstalk unraveled tight connection between communities development...