Aquaporin (AQP) proteins comprise a group of membrane intrinsic (MIPs) that are responsible for transporting water and other small molecules, which is crucial plant survival under stress conditions including salt stress. Despite the vital role AQPs, little known about them in cucumber (Cucumis sativus L.).In this study, we identified 39 aquaporin-encoding genes were separated by phylogenetic analysis into five sub-families (PIP, TIP, NIP, SIP, XIP). Their substrate specificity was then...

The amount of chemical fertilizer for vegetables is on the high level in China. use organic fertilizers to meet nutrient requirement crops will be an inevitable practice sustainable agriculture. In this study, we compared effects pig manure fertilizer, rabbit and yield, quality Brassica rapa var. Chinensis, soil physico-chemical properties microbial community by using two consecutive seasons three a pot experiment. results were as follows: (1) first season, fresh yield Chinensis applying was...

Trihelix transcription factors (TTF) are a class of light-responsive proteins with typical triple-helix structure (helix-loop-helix-loop-helix). Members this gene family play an important role in plant growth and development, especially various abiotic stress responses. Salix matsudana Koidz is allotetraploid ornamental forest tree that widely planted for its excellent resistance to stress, but no studies on have been reported. In study, the was analyzed at genome-wide level S. . A total 78...

Summary Steroidal glycoalkaloids (SGAs) constitute a characteristic class of antinutritional metabolites that are found in certain Solanum species. Despite the considerable studies on SGA biosynthesis, mechanisms crosstalk between hormone signaling pathways regulate content still remain to be elucidated. Here, we performed metabolic genome‐wide association study (mGWAS) based levels and identified SlERF.H6 as negative regulator bitter‐SGA biosynthesis. repressed expression biosynthetic...

Rubber (cis-1,4-polyisoprene), an important raw material for many industrial uses, is synthesized in the latex of Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg. We postulated that genes uniquely or preferentially expressed may be rubber biosynthesis. constructed cDNA libraries from H. to investigate by single-run partial sequencing clones. Sequence analyses identified 245 sequence tags (ESTs), which 57% showed homology previously described sequences public databases. About 16%...

From a high-salt extract of the purified thylakoid membrane, an 18-kD protein was detected. This translated by chloroplast ribosomes and could form stable DNA-protein complex with cloned DNA replicative origin [Nie, Z.Q., Chang, D.Y., Wu, M. (1987) Mol. Gen. Genet. 209, 265-269]. In this paper, is linked to frxB, chloroplast-encoded, ferredoxin-type, iron-sulfur protein, N-terminal microsequencing computer analysis. The identification further supported empirically fact that electron...

Background The hormone auxin plays an important role not only in the growth and development of rice, but also its defense responses. We've previously shown that P450 gene CYP71Z2 enhances disease resistance to pathogens through regulation phytoalexin biosynthesis though it remains unclear if is involved this process or not. Methodology Principal Findings expression was induced by Xanthomonas oryzae pv. (Xoo) inoculation analyzed qRT-PCR, with GUS histochemical staining showing limited roots,...

Rich endophytic bacterial communities exist in fescue (Festuca ovina L.) and play an important role growth, cold tolerance, drought tolerance antibiotic tolerance. To screen for probiotics carried by seeds, seven varieties were collected from three different regions of China isolation the milled seed method analyzed diversity motility, biofilm resistance. A total 91 isolates obtained, based on morphological characteristics, 36 representative dominant strains selected 16S rDNA sequencing...

ABSTRACT The accumulation of amyloid plaques is a pathological hallmark Alzheimer's disease (AD), in which ADAM10, the α‐secretase that catalyzes APP and facilitates non‐amyloidogenesis pathway, plays an important role. We have previously reported expression copine‐7 (Cpne7) hippocampus APP/PS1 mice significantly upregulated by nicotine, whereas potential role CPNE7 AD remains largely unknown. Here, we report protein levels are decreased HEK293 cells stably expressing full‐length APP. shown...

ABSTRACT Plants have evolved a sophisticated chemical defense network to counteract pathogens, with phenolamides and salicylic acid (SA) playing pivotal roles in the immune response. However, synergistic regulatory mechanisms of their biosynthesis remain be explored. Here, we identified biosynthetic gene cluster on chromosome 2 (BGC2) associated phenolamide SA, wherein key component SlEPS1 exhibits dual catalytic functions for synthesis SA. Overexpression BGC2 tomato enhanced resistance...

Abstract Background Carbapenem-resistant Acinetobacter baumannii (CRAB) poses a critical global threat, especially in ICUs. Yet, reports on ST164 CRAB harboring bla NDM-1 remain scarce. This study investigates two clinical isolates, L4773hy and L4796hy, derived from intestinal colonization Hangzhou, China, focusing their phenotypic genomic characteristics as well the broader transmission of A. . Methods Bacterial identification was performed using matrix-assisted laser desorption/ionization...

The sex determination in gymnosperms is still poorly characterized due to the lack of genomic/transcriptome resources and useful molecular genetic markers. To enhance our understanding mechanisms sexual recognition reproductive structures conifers, transcriptome male female conelets were a Chinese endemic conifer species, Pinus bungeana Zucc. ex Endl. 39.62 Gb high-throughput sequencing reads obtained from two kinds conelets. After de novo assembly reads, 85,305 unigenes identified, 53,944...

Abstract Parrotia subaequalis (Hamamelidaceae) is a Tertiary relic species endemic in eastern China. We used inter‐simple sequence repeat ( ISSR ) markers to access genetic diversity and population structure natural five populations of P. . The levels were higher at level H = 0.2031) but lower 0.1096). might be attributed the accumulation distinctive genotypes which adapted different habitats after Quaternary glaciations. Meanwhile, founder effects on early stage, subsequent bottleneck...

HSP70 protein, as an important member of the heat shock protein (HSP) family, plays role in plant growth, development, and response to biotic abiotic stresses. In order explore

Abstract Alligatorweed [ Alternanthera philoxeroides (Mart.) Griseb.] is an invasive semiaquatic weed that poses a serious threat to agricultural production and ecological balance worldwide. However, information about genetic factors associated with the adaptation invasion mechanisms of this species limited. Screening for appropriate reference genes important gene expression functional analysis research in A. . In study, 30 candidate showed stable different tissues under various treatments...

Fungi secrete numerous effectors to modulate host defense systems. Understanding the molecular mechanisms by which fungal regulate plant is of great importance for development novel strategies disease control. In this study, we identified necrosis- and ethylene-inducing protein 1 (Nep1)-like (NLP) effector gene, CgNLP1, contributed conidial germination, appressorium formation, invasive growth, virulence Colletotrichum gloeosporioides rubber tree. Transient expression CgNLP1 in leaves...

Glucoraphanin (GRA) is present in the seeds and nutrient organs of broccoli precursor anti-cancer compound sulforaphane (SF). The hairy roots obtained by infecting (Brassica oleracea L. var. Italic Planch) leaves with Agrobacterium rhizogenes (ATCC15834) are phytohormonally autonomous, genetically stable, can produce large amounts substance SF. Melatonin (MT) a natural hormone widely found plants. Studies have shown that melatonin regulate synthesis secondary metabolites downstream targets...

Mammalian cardiomyocytes may permanently lose their ability to proliferate after birth. Therefore, studying the proliferation and growth arrest of during postnatal period enhance current understanding regarding this molecular mechanism. The present study identified differentially expressed genes in hearts obtained from 24 h‑old mice, which contain proliferative cardiomyocytes; 7‑day‑old are undergoing a burst; 10‑week‑old growth‑arrested cardiomyocytes, using global gene expression analysis....

The microtubule cytoskeleton is a dynamic system that plays vital roles in fundamental cellular processes and responses to environmental stumili. Salt stress induced depolymerization reorganization of microtubules are believed function the promotion survival Arabidopsis. Microtubule-severing enzyme ATKATANIN1 (AtKTN1) recognized as MAP help maintain organized structure. To date, whether AtKTN1 involved response salt Arabidopsis remains unknown. Here, our phenotypic analysis showed...