A5079465456- Immune cells in cancer
- RNA Interference and Gene Delivery
- Lymphoma Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Vitamin D Research Studies
- Nanoparticle-Based Drug Delivery
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Nutrition and Health in Aging
- Chronic Kidney Disease and Diabetes
- Gold and Silver Nanoparticles Synthesis and Applications
- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
- Renal Diseases and Glomerulopathies
- Frailty in Older Adults
- Adenosine and Purinergic Signaling
- Trace Elements in Health
- Nanoplatforms for cancer theranostics
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Hippo pathway signaling and YAP/TAZ
- Heme Oxygenase-1 and Carbon Monoxide
- Immunotherapy and Immune Responses
Nankai University
2016-2025
Guangdong Academy of Medical Sciences
2022-2024
Guangdong Provincial People's Hospital
2022-2024
Southern Medical University
2023-2024
State Key Laboratory of Medicinal Chemical Biology
2016-2021
Indiana University School of Medicine
2013
Scripps Research Institute
2012
University of Pittsburgh
2006-2009
Abstract Legumain is required for maintenance of normal kidney homeostasis. However, its role in acute injury (AKI) still unclear. Here, we induced AKI by bilateral ischemia-reperfusion (IRI) renal arteries or folic acid lgmn WT and KO mice. We assessed serum creatinine, blood urea nitrogen, histological indexes tubular injury, expression KIM-1 NGAL. Inflammatory infiltration was evaluated immunohistological staining CD3 F4/80, TNF-α, CCL-2, IL-33, IL-1α. Ferroptosis Acsl4, Cox-2, reactive...
Rationale: Differential activation of macrophages correlates closely with tumor progression, and the epigenetic factor lysine demethylase 6B (KDM6B, previously named JMJD3) mediates regulation macrophage polarization through an unknown mechanism. Methods: We developed a suspension coculture system comprising breast cancer cells used RT-qPCR western blotting to measure KDM6B expression. Bioinformatics luciferase reporter assays were identify candidate microRNAs responsible for downregulation...
Deficiency in vitamin D and its active metabolites is a pathologic feature of chronic kidney diseases. Despite that tubular epithelial cells are the major sites synthesis, little known about role maintaining structural functional integrity epithelium. This study investigated effects paricalcitol (19-nor-1,25-hydroxy-vitamin D<sub>2</sub>), synthetic analogue, on obstructive nephropathy, model characterized by predominant tubulointerstitial lesions. Compared with vehicle controls,...
Inflammation is a pathologic feature of variety chronic kidney diseases. Several lines evidence suggest potential anti-inflammatory role for vitamin D in disease, but the underlying mechanism remains unknown. Here, effect synthetic analogue paricalcitol on renal inflammation was investigated mouse model obstructive nephropathy. Paricalcitol reduced infiltration T cells and macrophages obstructed kidney. This inhibition inflammatory cell accompanied by decreased expression RANTES TNF-α....
Legumain-based DNA vaccines have potential to protect against breast cancer. However, the lack of a safe and efficient oral delivery system restricts its clinical application. Here, we constructed alginic acid-coated chitosan nanoparticles (A.C.NPs) as an carrier for legumain vaccine. First, tested characteristic in acidic environments vitro. agarose electrophoresis data show that A.C.NPs protected better from degradation solution (pH 1.5) than did (C.NPs). Furthermore, size distribution...
Abstract Effects of M2 tumour-associated macrophages on the pathogenesis diffuse large B cell lymphoma (DLBCL) are still controversial. Our data showed that number CD163-positive correlated negatively with DLBCL prognosis. Macrophage depletion by clodronate liposomes significantly suppressed tumour growth in a xenograft mouse model using OCI-Ly3 cells. Moreover, polarization induced legumain expression U937 Exogenous promoted degradation fibronectin and collagen I, which was abolished...
Abstract The prognosis of renal cell carcinoma (RCC) remains poor due to metastases and resistance chemotherapy. Salinomycin (Sal) exhibits the potential antitumor, while underlying mechanism is not completely clear. Here, we found that Sal induced ferroptosis in RCCs identified Protein Disulfide Isomerase Family A Member 4 (PDIA4) as a mediator Sal’s effect on ferroptosis. suppressed PDIA4 by increasing its autophagic degradation. Downregulation increased sensitivity ferroptosis, ectopic...
The clinical efficacy of neoadjuvant immunotherapy plus chemotherapy remains elusive in localized epidermal growth factor receptor (EGFR)-mutant non-small cell lung cancer (NSCLC). Here, we report interim results a Simon's two-stage design, phase 2 trial using sintilimab with carboplatin and nab-paclitaxel resectable EGFR-mutant NSCLC. All 18 patients undergo radical surgery, one patient experiencing surgery delay. Fourteen exhibit confirmed radiological response, 44% achieving major...
Triple-negative breast cancer (TNBC) is the most malignant cancer, with high rates of relapse and metastasis. Because nonspecific targeting chemotherapy insurmountable aggressiveness, TNBC therapy lacks an effective strategy. Exosomes have been reported as efficient drug delivery system (DDS). CD82 a tumor metastasis inhibitory molecule that enriched in exosomes. Aptamer AS1411 specifically targets cells due to its expression nucleolin. We generated "triple-punch" cell membrane-derived...
Integrin-linked kinase (ILK) is an intracellular serine/threonine protein that regulates cell adhesion, survival, and epithelial-to-mesenchymal transition (EMT). In this study, we investigated the activity of ILK during tubular EMT induced by TGF-beta1 examined therapeutic potential inhibitor in obstructive nephropathy. a biphasic activation renal epithelial cells, with rapid starting at 5 min second wave peaking 24 h; latter paralleled induction expression. Pharmacologic inhibition...
Background: Tetraspanins constitute a family of transmembrane spanning proteins that function mainly by organizing the plasma membrane into micro-domains. CD82, member tetraspanins, is potent inhibitor cancer metastasis in numerous malignancies. CD82 highly glycosylated protein, however, it still unknown whether and how this post-translational modification affects metastasis. Methods: The glycosylation profiles are checked paired human ovarian primary metastatic tissues. functional studies...
The yes-associated protein (YAP) transcription co-activator has been reported either as an oncogene candidate or a tumor suppressor. Liver tissue chips revealed that about 51.4% human hepatocellular carcinoma (HCC) samples express YAP and 32.9% HCC phosphorylated YAP. In this study, we found chemotherapy increased expression nuclear translocation in HepG2 cells, well p53 translocation. However, little is known functions during chemotherapy. Our results show overexpression of increases...
Both peritubular inflammation and tubular epithelial-to-mesenchymal transition (EMT) are critical events during the pathogenesis of renal fibrosis. However, relationship between these two processes is unclear. Here, we investigated potential role vitamin D receptor (VDR) in coupling EMT. In a mouse model unilateral ureteral obstruction (UUO), loss VDR was observed as early 1 day after surgery. cultured proximal epithelial HK-2 cells, proinflammatory TNF-α inhibited expression dose-...
Abstract Aging is an independent risk factor for acute kidney injury and subsequent chronic diseases, while the underlying mechanism still elusive. Here, we found that renal tubules highly express a conserved lysosomal endopeptidase, legumain, which significantly downregulated with growing of age. Tubule‐specific legumain‐knockout mice exhibit spontaneous interstitial fibrosis from 3rd month. In tubule‐specific cultured legumain‐knockdown HK‐2 cells, legumain deficiency induces activation...
Activation and expansion of interstitial fibroblasts myofibroblasts play an essential role in the evolution renal fibrosis. After obstructive injury, mice lacking tissue-type plasminogen activator (tPA) have fewer less fibrosis than wild-type controls. This suggests that tPA controls size fibroblast/myofibroblast population vivo, this study sought to determine underlying mechanism. In vitro, inhibited staurosporine or H(2)O(2)-induced caspase-3 activation, prevented cellular DNA...
The relationship between tumor-associated macrophages (TAM) and epithelial-to-mesenchymal transition (EMT) during the initiation progression of metastasis is still unclear. Here, a role for vitamin D receptor (VDR) in was identified, as well TAMs EMT. First, expression level VDR examined clinical tissue from human patients with breast cancer or mouse model differential metastasis. These results revealed that negatively correlates cancer. Second, coculture VDR-overexpressing cells macrophage...