A5101732508- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Regulation of Appetite and Obesity
- Circular RNAs in diseases
- Biochemical Analysis and Sensing Techniques
- Adipose Tissue and Metabolism
- Liver physiology and pathology
- Mathematical and Theoretical Epidemiology and Ecology Models
- Drug-Induced Hepatotoxicity and Protection
- Autophagy in Disease and Therapy
- Hepatocellular Carcinoma Treatment and Prognosis
- Wound Healing and Treatments
- Inflammatory mediators and NSAID effects
- Evolution and Genetic Dynamics
- Head and Neck Anomalies
- Helicobacter pylori-related gastroenterology studies
- COVID-19 epidemiological studies
- Thyroid Cancer Diagnosis and Treatment
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Ion Channels and Receptors
- Burn Injury Management and Outcomes
- Barrier Structure and Function Studies
- Respiratory and Cough-Related Research
Zhejiang University
2023
China Academy of Space Technology
2023
Sir Run Run Shaw Hospital
2023
Jinshan Hospital of Fudan University
2013-2022
Guilin Medical University
2022
Zhejiang Provincial People's Hospital
2011-2021
Hangzhou Medical College
2018-2021
Second Hospital of Hebei Medical University
2020
Anyang Institute of Technology
2019-2020
Hebei Medical University
2020
Abstract The aim of this study was to investigate the effect quercetin on hepatic fibrosis, a characteristic response acute or chronic liver injury. Mice were randomized bile duct ligation (BDL) carbon tetrachloride (CCl 4 ) cirrhosis models. Quercetin (100 mg/kg 200 daily) administered by gavage for 2 weeks. Liver tissue and blood samples collected histological molecular analysis. results our experiments showed that reduced BDL CCl inhibited extracellular matrix formation, regulated...
Abstract Objective Hepatocellular carcinima is one of the most common tumors in clinic and also leading causes death from cancer worldwide. Quercetin shows significant effects on blocking development various cancers. Methods We used human hepatocellular carcinoma LM3 nude mice tumor model to assess quercetin clarify its mechanism action. collected cell line treated with different doses at time periods determined vital indexes. The liver tissues were for western boltting (WB), Hematoxylin...
Genistein is a natural isoflavone with many health benefits, including antitumour effects. Increased hypoxia-inducible factor 1 α (HIF-1α) levels and glycolysis in tumour cells are associated an increased risk of mortality, cancer progression, resistance to therapy. However, the effect genistein on HIF-1α hepatocellular carcinoma (HCC) still unclear. Cell viability, apoptosis rate, lactate production, glucose uptake were measured HCC cell lines incubation. Lentivirus-expressed transporter...
Hepatocellular carcinoma (HCC) is one of the few cancers with a continuous increase in incidence and mortality. Drug resistance major problem treatment HCC. In this study, two sorafenib‐resistant HCC cell lines nude mouse subcutaneously tumor model were used to explore possible mechanisms leading sorafenib resistance, investigate whether aspirin could sensitivity hepatoma cells sorafenib. The combination resulted synergistic antitumor effect against liver tumors both vitro vivo . High...
Increased tight junction (TJ) barrier permeability, induced by tumour necrosis factor (TNF)-α, may lead to the defects in TJ and subsequent development of inflammation. Recent evidence suggests that miR-21 is implicated inflammatory diseases. However, physiological role intestinal permeability remains elusive. This study aimed explore epithelial permeability. The filter-grown Caco-2 monolayers model system was established mimic defect. proteins were detected immunofluorescence western blot...
Background/Aims: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic worldwide. Autophagy associated with NAFLD. Ghrelin a gut hormone various functions including energy metabolism and inflammation inhibition. We investigated therapeutic effect of ghrelin on NAFLD its association autophagy. Methods: C57bl/6 mice were fed high-fat diet for 8 weeks to induce model NAFLD, (10 µg/kg) administrated subcutaneously twice weekly from 6 8. LO2 cells pretreated (10-8 M) before...
Abstract Background Liver fibrosis is a wound‐healing process of liver featured by the over‐deposition extracellular matrix (ECM) and angiogenesis. However, effective treatment lacking. Procyanidin B2 (PB2) flavonoid extract abundant in grape seeds with anti‐oxidant, anti‐inflammatory anti‐cancer properties. The present study aimed to determine effects PB2 on fibrosis. Method CCl4‐induced mouse model human hepatic stellate cell (HSC) line (LX2 cells) were used activation, ECM production...
Ghrelin is a stomach-derived growth hormone secretagogue that promotes various physiological effects, including energy metabolism and amelioration of inflammation. The purpose this study was to investigate the protective mechanism ghrelin against liver fibrosis. Liver fibrosis induced in C57BL/6 mice by intraperitoneal injection CCl4 (2.0 mL/kg 10% v/v solution peanut oil) two times per week for eight weeks. (10 μg/kg) intraperitoneally injected A second murine model bile duct ligation (BDL)...
Abstract: Ischemia–reperfusion injury (IRI) contributes to liver damage in many clinical situations, such as resection and transplantation. In the present study, we investigated effects of antioxidant, anti-inflammatory, anticancer agent salidroside (Sal) on hepatic IRI mice. The mice were randomly divided into six groups: normal control, Sham, Sal (20 mg/kg), IRI, + (10 mg/kg). We measured enzymes, proinflammatory cytokines, TNF-α interleukin-6, apoptosis- autophagy-related marker proteins...
Emerging evidence shows that the Hedgehog pathway and long noncoding RNA TUG1 play pivotal roles in cell proliferation, migration, invasion tumors. However, mechanism underlying effect of hepatoma remains undefined. In present study, we showed expression was negatively correlated with microRNA (miR)-132, depletion inhibited activation vitro vivo. We functions as a competing endogenous (ceRNA) by miR-132 for binding to sonic hedgehog protein HCC, thereby suppressing signaling its tumorigenic...
Shikonin is a natural product with many activities, including anti-cancer effects. Pyruvate kinase type M2 (PKM2) plays crucial role in the growth of tumor cells. However, effect shikonin on PKM2 hepatocellular carcinoma (HCC) unclear.Cell viability, apoptosis level, glucose uptake, and lactate production were detected HCC Lentivirus-overexpressed -shRNA used to verify key target shikonin. A xenograft mouse model was detect efficacy its combination sorafenib vivo.Shikonin inhibited...
Abstract Liver fibrosis, a consequence of unhealthy modern lifestyles, has growing impact on human health, particularly in developed countries. Here, we have explored the anti‐fibrotic effects propylene glycol alginate sodium sulphate (PSS), natural extract from brown algae, fibrotic mice and cell models. Thus, established bile duct ligature carbon tetrachloride mouse models LX‐2 with or without PSS treatment. pathological sections relevant indicators serum liver tissues were examined....
Non-alcoholic fatty liver disease (NAFLD) can be attributed to the dysregulation of hepatic lipid metabolism; however, its cellular and molecular mechanisms remain unclear. This study aims explore effect long non-coding RNA growth arrest specific 5 (GAS5) on metabolism in models.Obese mice, high fat diet-fed mice free acid-stimulated cells were used for GAS5 expression detection. overexpression or knockdown models established elucidate regulatory function de novo lipogenesis (DNL)...
Liver fibrosis is a worldwide clinical challenge during the progression of chronic liver disease to cirrhosis. Shikonin extracted from root Lithospermum erythrorhizon with antioxidant, anti-inflammatory, anticancer, and wound-healing properties. The study aims investigate protective effect shikonin on its underlying mechanism.Two models were established in male C57 mice by intraperitoneal injection CCl4 or bile duct ligation. was administered orally three times weekly at dose 2.5 5 mg/kg....
Hepatic ischemia reperfusion (IR) injury (IRI) occurs during liver transplantation, hepatectomy, and hemorrhagic shock. Oleanolic acid (OA) is a natural compound with antioxidant anti-inflammatory activity that has been used to treat disorders in clinical practice for several years. Here, we investigated the effects underlying mechanisms of OA hepatic IRI. A 60-minute partial (70%) hepatic, warm, ischemic model was established BALB/c mice, two doses (30 60 mg/kg) were administered...
Recent advances in next‑generation sequencing technology transcriptome analysis have helped identify numerous non-coding RNAs. The long RNA (lncRNA) is commonly defined as an molecule with a length of 200 bp-100 kbp that lacks protein‑coding potential. LncRNAs play critical role the regulation gene expression, including chromatin modification, transcription and post‑transcriptional processing. It has been confirmed dysregulation lncRNAs associated number human diseases, particularly tumors....
Ghrelin is a 28-amino-acid gut hormone that was first discovered as potent growth secretagogue. Recently, it has been shown to exert strong anti-inflammatory effect. The purpose of the study reported here explore effect and mechanism ghrelin on concanavalin (Con) A-induced acute hepatitis.Balb/C mice were divided into four groups: normal control (NC) (mice injected with vehicle [saline]); Con A (25 mg/kg); + 10 μg/kg ghrelin; 50 (1 hour before injection). Pro-inflammatory cytokine levels...
Background . Hepatic ischemia reperfusion (IR) injury is a common phenomenon in transplantation or trauma. The aim of the present study was to determine protective effect quercetin (QE) on hepatic IR via ERK/NF- κ B pathway. Methods Mice were randomized into sham, IR, QE100 + and QE200 groups. Quercetin administered intragastrically daily at two doses (100 mg/kg 200 mg/kg) for 5 days prior injury. expression levels liver enzymes, inflammatory cytokines, other marker proteins determined 2, 8,...